Actinomadura graeca sp. nov.: A novel producer of the macrocyclic antibiotic zelkovamycin

Tarantini, Francesco Saverio; Brunati, Mara; Taravella, Anna; Carrano, Lucia; Parenti, Francesco; Hong, Kar Wai; Williams, Paul; Chan, Kok Gan; Heeb, Stephan; Chan, Weng C.

Abstract

As part of a screening programme for antibiotic-producing bacteria, a novel Actinomadura species was discovered from a soil sample collected in Santorini, Greece. Preliminary 16S rRNA gene sequence comparisons highlighted Actinomadura macra as the most similar characterised species. However, whole-genome sequencing revealed an average nucleotide identity (ANI) value of 89% with A. macra, the highest among related species. Further phenotypic and chemotaxonomic analyses confirmed that the isolate represents a previously uncharacterised species in the genus Actinomadura, for which the name Actinomadura graeca sp. nov. is proposed (type strain 32-07T). The G+C content of A. graeca 32-07 is 72.36%. The cell wall contains DL-diaminopimelic acid, intracellular sugars are glucose, ribose and galactose, the predominant menaquinone is MK-9(H6), the major cellular lipid is phosphatidylinositol and fatty acids consist mainly of hexadecanoic acid. No mycolic acid was detected. Furthermore, A. graeca 32-07 has been confirmed as a novel producer of the non-ribosomal peptide antibiotic zelkovamycin and we report herein a provisional description of the unique biosynthetic gene cluster.

Citation

Tarantini, F. S., Brunati, M., Taravella, A., Carrano, L., Parenti, F., Hong, K. W., Williams, P., Chan, K. G., Heeb, S., & Chan, W. C. (2021). Actinomadura graeca sp. nov.: A novel producer of the macrocyclic antibiotic zelkovamycin. PLoS ONE, 16(11), Article e0260413. https://doi.org/10.1371/journal.pone.0260413

• Journal Article Type: Article
• Acceptance Date: Nov 9, 2021
• Online Publication Date: Nov 30, 2021
• Publication Date: Nov 1, 2021
• Deposit Date: Nov 30, 2021
• Publicly Available Date: Dec 6, 2021
• Journal: PLoS ONE
• Electronic ISSN: 1932-6203
• Publisher: Public Library of Science
• Peer Reviewed: Peer Reviewed
• Volume: 16
• Issue: 11
• Article Number: e0260413
• DOI: https://doi.org/10.1371/journal.pone.0260413
• Keywords: Multidisciplinary
• Public URL: https://nottingham-repository.worktribe.com/output/6842832
• Publisher URL: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0260413