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Generation of Doubled Haploid Wheat-Triticum urartu Introgression Lines and Their Characterisation Using Chromosome-Specific KASP Markers

Grewal, Surbhi; Guwela, Veronica; Newell, Claire; Yang, Cai Yun; Ashling, Stephen; Scholefield, Duncan; Hubbart-Edwards, Stella; Burridge, Amanda; Stride, Alex; King, Ian P.; King, Julie

Generation of Doubled Haploid Wheat-Triticum urartu Introgression Lines and Their Characterisation Using Chromosome-Specific KASP Markers Thumbnail


Authors

Veronica Guwela

Claire Newell

Cai Yun Yang

Stephen Ashling

Duncan Scholefield

Amanda Burridge

Alex Stride

Ian P. King



Abstract

Wheat is one of the most important food and protein sources in the world and although, in recent years wheat breeders have achieved yield gains, they are not sufficient to meet the demands of an ever-growing population. Development of high yielding wheat varieties, resilient to abiotic and biotic stress resulting from climate change, has been limited by wheat’s narrow genetic base. In contrast to wheat, the wild relatives of wheat provide a vast reservoir of genetic variation for most, if not all, agronomic traits. Previous studies by the authors have shown the transfer of genetic variation from T. urartu into bread wheat. However, before the introgression lines can be exploited for trait analysis, they are required to have stable transmission of the introgressions to the next generation. In this work, we describe the generation of 86 doubled haploid (DH) wheat-T. urartu introgression lines that carry homozygous introgressions which are stably inherited. The DH lines were characterised using the Axiom® Wheat Relative Genotyping Array and 151 KASP markers to identify 65 unique T. urartu introgressions in a bread wheat background. DH production has helped accelerate the breeding process and facilitated the early release of homozygous wheat-T. urartu introgression lines. Together with the KASP markers, this valuable resource could greatly advance identification of beneficial alleles that can be used in wheat improvement.

Citation

Grewal, S., Guwela, V., Newell, C., Yang, C. Y., Ashling, S., Scholefield, D., Hubbart-Edwards, S., Burridge, A., Stride, A., King, I. P., & King, J. (2021). Generation of Doubled Haploid Wheat-Triticum urartu Introgression Lines and Their Characterisation Using Chromosome-Specific KASP Markers. Frontiers in Plant Science, 12, Article 643636. https://doi.org/10.3389/fpls.2021.643636

Journal Article Type Article
Acceptance Date Apr 19, 2021
Online Publication Date May 13, 2021
Publication Date May 13, 2021
Deposit Date May 5, 2021
Publicly Available Date May 13, 2021
Journal Frontiers in Plant Science
Electronic ISSN 1664-462X
Publisher Frontiers Media
Peer Reviewed Peer Reviewed
Volume 12
Article Number 643636
DOI https://doi.org/10.3389/fpls.2021.643636
Keywords wheat; Triticum urartu; doubled haploids; introgressions; KASP markers; genotyping 10
Public URL https://nottingham-repository.worktribe.com/output/5509632
Publisher URL https://www.frontiersin.org/articles/10.3389/fpls.2021.643636/full

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