Efficient Amino Donor Recycling in Amination Reactions: Development of a New Alanine Dehydrogenase in Continuous Flow and Dialysis Membrane Reactors

Roura Padrosa, David; Nisar, Zoya; Paradisi, Francesca

Abstract

Transaminases have arisen as one of the main biocatalysts for amine production but despite their many advantages, their stability is still a concern for widespread application. One of the reasons for their instability is the need to use an excess of the amino donor when trying to synthetize amines with unfavourable equilibria. To circumvent this, recycling systems for the amino donor, such as amino acid dehydrogenases or aldolases, have proved useful to push the equilibria while avoiding high amino donor concentrations. In this work, we report the use of a new alanine dehydrogenase from the halotolerant bacteria Halomonas elongata which exhibits excellent stability to different cosolvents, combined with the well characterised CbFDH as a recycling system of L¬-alanine for the amination of three model substrates with unfavourable equilibria. In a step forward, the amino donor recycling system has been co-immobilised and used in flow with success as well as re-used as a dialysis enclosed system for the amination of an aromatic aldehyde.

Citation

Roura Padrosa, D., Nisar, Z., & Paradisi, F. (2021). Efficient Amino Donor Recycling in Amination Reactions: Development of a New Alanine Dehydrogenase in Continuous Flow and Dialysis Membrane Reactors. Catalysts, 11(4), Article 520. https://doi.org/10.3390/catal11040520

• Journal Article Type: Article
• Acceptance Date: Apr 15, 2021
• Online Publication Date: Apr 20, 2021
• Publication Date: Apr 20, 2021
• Deposit Date: Apr 21, 2021
• Publicly Available Date: Apr 21, 2021
• Journal: Catalysts
• Electronic ISSN: 2073-4344
• Publisher: MDPI
• Peer Reviewed: Peer Reviewed
• Volume: 11
• Issue: 4
• Article Number: 520
• DOI: https://doi.org/10.3390/catal11040520
• Keywords: Physical and Theoretical Chemistry; Catalysis
• Public URL: https://nottingham-repository.worktribe.com/output/5486472
• Publisher URL: https://www.mdpi.com/2073-4344/11/4/520