Sutham Suthaporn
Evaluating the influence of progesterone concentration and time of exposure on in vitro endometrial decidualisation
Suthaporn, Sutham; Jayaprakasan, Kanna; Thornton, Jim G.; Walker, Kate F.; Castellanos, Marcos; May, Sean; Hernandez-Medrano, Juan; Maalouf, Walid E.
Authors
Kanna Jayaprakasan
Jim G. Thornton
KATE WALKER Kate.Walker@nottingham.ac.uk
Clinical Professor
Marcos Castellanos
Professor SEAN MAY SEAN.MAY@NOTTINGHAM.AC.UK
Professor of Plant Cyber Infrastructure
Juan Hernandez-Medrano
Walid E. Maalouf
Abstract
This study aimed to evaluate the influence of progesterone (concentration and time of exposure) on endometrial decidualisation using an in vitro model cell line: Human Endometrial Stromal Cells (HESCs). HESCs exposed to progesterone (1 and 10 μM) had higher percentages of decidualised cells and higher expression of the decidual marker (Insulin Like Growth Factor Binding Protein 1 (IGFBP1)) compared with those exposed to (0.1 μM). Among those HESCs cultured with 1 μM progesterone for 11 days, the highest rate of morphological differentiation (40–50%) occurred between days 7–9 and IGFBP1 peaked on day 7. The cell-cycle pathway was significantly down-regulated in HESCs exposed to at least 1 μM progesterone regardless of the incubation period. We conclude that exposure to high progesterone concentration for 7–9 days is essential to maximise the process of decidualisation.
Citation
Suthaporn, S., Jayaprakasan, K., Thornton, J. G., Walker, K. F., Castellanos, M., May, S., …Maalouf, W. E. (2021). Evaluating the influence of progesterone concentration and time of exposure on in vitro endometrial decidualisation. Molecular and Cellular Endocrinology, 528, Article 111242. https://doi.org/10.1016/j.mce.2021.111242
Journal Article Type | Article |
---|---|
Acceptance Date | Mar 8, 2021 |
Online Publication Date | Mar 11, 2021 |
Publication Date | May 15, 2021 |
Deposit Date | Oct 13, 2021 |
Publicly Available Date | Mar 12, 2022 |
Journal | Molecular and Cellular Endocrinology |
Print ISSN | 0303-7207 |
Electronic ISSN | 1872-8057 |
Publisher | Elsevier |
Peer Reviewed | Peer Reviewed |
Volume | 528 |
Article Number | 111242 |
DOI | https://doi.org/10.1016/j.mce.2021.111242 |
Public URL | https://nottingham-repository.worktribe.com/output/5481125 |
Publisher URL | https://www.sciencedirect.com/science/article/pii/S0303720721000861?via%3Dihub |
Files
Cell Line (Revision 2.3)
(503 Kb)
PDF
You might also like
Childbirth experience questionnaire: validating its use in the United Kingdom
(2015)
Journal Article
Randomized trial of labor induction in women 35 years of age or older
(2016)
Journal Article
Labour induction near term for women aged 35 or over: an economic evaluation
(2017)
Journal Article
Downloadable Citations
About Repository@Nottingham
Administrator e-mail: discovery-access-systems@nottingham.ac.uk
This application uses the following open-source libraries:
SheetJS Community Edition
Apache License Version 2.0 (http://www.apache.org/licenses/)
PDF.js
Apache License Version 2.0 (http://www.apache.org/licenses/)
Font Awesome
SIL OFL 1.1 (http://scripts.sil.org/OFL)
MIT License (http://opensource.org/licenses/mit-license.html)
CC BY 3.0 ( http://creativecommons.org/licenses/by/3.0/)
Powered by Worktribe © 2024
Advanced Search