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Marine-Source Quorum Quenching Enzyme YtnP to Improve Hygiene Quality in Dental Units

Sun, Xiaohui; Hill, Philip; Liu, Jia; Qian, Jing; Ma, Yuting; Zhou, Shufeng

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Authors

Xiaohui Sun

PHIL HILL phil.hill@nottingham.ac.uk
Associate Professor

Jia Liu

Jing Qian

Yuting Ma

Shufeng Zhou



Abstract

Biofilm in dental unit water lines may pose a health risk to patients and dental practitioners. An AdiC-like quorum quenching enzyme, YtnP, was cloned from a deep-sea probiotic Bacillus velezensis, and heterologously expressed in E. coli to examine the application on the improvement of hygiene problems caused by biofilm infection of Pseudomonas aeruginosa in dental units. Pseudomonas bacteria were isolated from dental chair units and used to grow static biofilms in the laboratory. A water filter system was designed to test the antifouling activity of YtnP in Laboratory, to simulate the biofilm contamination on water filter in dental unit water lines. The results demonstrated that the enzyme of YtnP was able to degrade the N-acyl homoserine lactones, significantly inhibited the EPS generation, biofilm formation, and virulence factors production (pyocyanin and rhamnolipid) of P. aeruginosa, and was efficient on the antifouling against P. aeruginosa. The findings in this study indicated the possibility of YtnP as novel disinfectant reagent for hygiene treatment in dental units.

Citation

Sun, X., Hill, P., Liu, J., Qian, J., Ma, Y., & Zhou, S. (2021). Marine-Source Quorum Quenching Enzyme YtnP to Improve Hygiene Quality in Dental Units. Marine Drugs, 19(4), Article 225. https://doi.org/10.3390/md19040225

Journal Article Type Article
Acceptance Date Mar 26, 2021
Online Publication Date Apr 16, 2021
Publication Date Apr 16, 2021
Deposit Date Apr 17, 2021
Publicly Available Date Apr 18, 2021
Journal Marine drugs
Electronic ISSN 1660-3397
Publisher MDPI
Peer Reviewed Peer Reviewed
Volume 19
Issue 4
Article Number 225
DOI https://doi.org/10.3390/md19040225
Keywords Drug Discovery
Public URL https://nottingham-repository.worktribe.com/output/5470045
Publisher URL https://www.mdpi.com/1660-3397/19/4/225

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