Marine-Source Quorum Quenching Enzyme YtnP to Improve Hygiene Quality in Dental Units

Sun, Xiaohui; Hill, Philip; Liu, Jia; Qian, Jing; Ma, Yuting; Zhou, Shufeng

Abstract

Biofilm in dental unit water lines may pose a health risk to patients and dental practitioners. An AdiC-like quorum quenching enzyme, YtnP, was cloned from a deep-sea probiotic Bacillus velezensis, and heterologously expressed in E. coli to examine the application on the improvement of hygiene problems caused by biofilm infection of Pseudomonas aeruginosa in dental units. Pseudomonas bacteria were isolated from dental chair units and used to grow static biofilms in the laboratory. A water filter system was designed to test the antifouling activity of YtnP in Laboratory, to simulate the biofilm contamination on water filter in dental unit water lines. The results demonstrated that the enzyme of YtnP was able to degrade the N-acyl homoserine lactones, significantly inhibited the EPS generation, biofilm formation, and virulence factors production (pyocyanin and rhamnolipid) of P. aeruginosa, and was efficient on the antifouling against P. aeruginosa. The findings in this study indicated the possibility of YtnP as novel disinfectant reagent for hygiene treatment in dental units.

Citation

Sun, X., Hill, P., Liu, J., Qian, J., Ma, Y., & Zhou, S. (2021). Marine-Source Quorum Quenching Enzyme YtnP to Improve Hygiene Quality in Dental Units. Marine Drugs, 19(4), Article 225. https://doi.org/10.3390/md19040225

• Journal Article Type: Article
• Acceptance Date: Mar 26, 2021
• Online Publication Date: Apr 16, 2021
• Publication Date: Apr 16, 2021
• Deposit Date: Apr 17, 2021
• Publicly Available Date: Apr 18, 2021
• Journal: Marine drugs
• Electronic ISSN: 1660-3397
• Publisher: MDPI
• Peer Reviewed: Peer Reviewed
• Volume: 19
• Issue: 4
• Article Number: 225
• DOI: https://doi.org/10.3390/md19040225
• Keywords: Drug Discovery
• Public URL: https://nottingham-repository.worktribe.com/output/5470045
• Publisher URL: https://www.mdpi.com/1660-3397/19/4/225