Clinical integration of fast Raman spectroscopy for Mohs micrographic surgery of basal cell carcinoma

Boitor, Radu; de Wolf, Coen; Weesie, Frank; Shipp, Dustin W.; Varma, Sandeep; Veitch, David; Wernham, Aaron; Koloydenko, Alexey; Puppels, Gerwin; Nijsten, Tamar; Williams, Hywel C.; Caspers, Peter; Notingher, Ioan

Abstract

We present the first clinical integration of a prototype device based on integrated auto-fluorescence imaging and Raman spectroscopy (Fast Raman device) for intra-operative assessment of surgical margins during Mohs micrographic surgery of basal cell carcinoma (BCC). Fresh skin specimens from 112 patients were used to optimise the tissue pre-processing and the Fast Raman algorithms to enable an analysis of complete Mohs layers within 30 minutes. The optimisation allowed >95% of the resection surface area to be investigated (including the deep and epidermal margins). The Fast Raman device was then used to analyse skin layers excised from the most relevant anatomical sites (nose, temple, eyelid, cheek, forehead, eyebrow and lip) and to detect the three main types of BCC (nodular, superficial and infiltrative). These results suggest that the Fast Raman technique is a promising tool to provide an objective diagnosis “tumour clear yes/no” during Mohs surgery of BCC. This clinical integration study is a key step towards a larger scale diagnosis test accuracy study to reliably determine the sensitivity and specificity in a clinical setting.

Citation

Boitor, R., de Wolf, C., Weesie, F., Shipp, D. W., Varma, S., Veitch, D., Wernham, A., Koloydenko, A., Puppels, G., Nijsten, T., Williams, H. C., Caspers, P., & Notingher, I. (2021). Clinical integration of fast Raman spectroscopy for Mohs micrographic surgery of basal cell carcinoma. Biomedical Optics Express, 12(4), 2015-2026. https://doi.org/10.1364/boe.417896

• Journal Article Type: Article
• Acceptance Date: Mar 1, 2021
• Online Publication Date: Mar 11, 2021
• Publication Date: Apr 1, 2021
• Deposit Date: Jun 17, 2021
• Publicly Available Date: Jun 17, 2021
• Journal: Biomedical Optics Express
• Electronic ISSN: 2156-7085
• Publisher: Optical Society of America
• Peer Reviewed: Peer Reviewed
• Volume: 12
• Issue: 4
• Pages: 2015-2026
• DOI: https://doi.org/10.1364/boe.417896
• Keywords: Biotechnology; Atomic and Molecular Physics, and Optics
• Public URL: https://nottingham-repository.worktribe.com/output/5394422
• Publisher URL: https://www.osapublishing.org/boe/fulltext.cfm?uri=boe-12-4-2015&id=449141
• Additional Information: This article is maintained by: OSA - The Optical Society; Crossref DOI link to publisher maintained version: https://doi.org/10.1364/BOE.417896; Article type: research-article; Similarity check: Screened by Similarity Check; Peer reviewed: Yes; Review process: Single blind; Received: 16 December 2020; Accepted: 1 March 2021; Published: 11 March 2021; Copyright: Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article's title, journal citation, and DOI.