Development, Implementation and Testing of a Multicellular Dynamic Action Potential Clamp Simulator for Drug Cardiac Safety Assessment

Camporesi, Maria; Bartolucci, Chiara; Lei, Chon Lok; Mirams, Gary R.; De Boer, Teun P.; Severi, Stefano

Abstract

© 2020 Creative Commons; the authors hold their copyright. As drugs can be multichannel blockers it is important to assess their cardiac safety taking into account multiple currents. In silico action potential (AP) models have been proposed for being able to integrate drugs effect on ionic currents and generate the resulting AP. However, a mathematical description of drug effects is required, which could be inaccurate. Dynamic Clamp has been proposed for drug cardiac safety assessment. In the dynamic action potential clamp (dAPC) configuration it creates an hybrid model connecting a real cell with a computer simulation. This way, drugs could be administrated directly to real cells, and effects on currents can be taken into account when generating the AP. Here we design and simulate a parallel multichannel dAPC system. The system includes the real cells overexpressing the currents of interest, the voltage clamp acquisition system, and the AP in silico model.

Citation

Camporesi, M., Bartolucci, C., Lei, C. L., Mirams, G. R., De Boer, T. P., & Severi, S. (2020, September). Development, Implementation and Testing of a Multicellular Dynamic Action Potential Clamp Simulator for Drug Cardiac Safety Assessment. Presented at Computing in Cardiology, Rimini, Italy

• Presentation Conference Type: Conference Paper (published)
• Conference Name: Computing in Cardiology
• Start Date: Sep 13, 2020
• End Date: Sep 16, 2020
• Acceptance Date: Sep 13, 2020
• Publication Date: Sep 16, 2020
• Deposit Date: Dec 22, 2021
• Publicly Available Date: Jan 6, 2022
• Publisher: Institute of Electrical and Electronics Engineers
• Volume: 47 (2020)
• ISBN: 9781728173825
• DOI: https://doi.org/10.22489/CinC.2020.416
• Public URL: https://nottingham-repository.worktribe.com/output/5376550
• Publisher URL: https://www.cinc.org/archives/2020/pdf/CinC2020-416.pdf
• Related Public URLs: https://cinc.org/archives/2020/