Acetylome analysis of the feline small intestine following Toxoplasma gondii infection

Meng, Yu-Meng; Zhai, Bin-Tao; Elsheikha, Hany M.; Xie, Shi-Chen; Wang, Ze-Xiang; Zhao, Quan; Zhu, Xing-Quan; He, Jun-Jun

Abstract

Toxoplasma gondii is a protozoan parasite capable of infecting a large number of warm-blooded animals and causes serious health complications in immunocompromised patients. T. gondii infection of the feline small intestine is critical for the completion of the life cycle and transmission of T. gondii. Protein acetylation is an important posttranslational modification, which plays roles in the regulation of various cellular processes. Therefore, understanding of how T. gondii reprograms the protein acetylation status of feline definitive host can help to thwart the production and spread of T. gondii. Here, we used affinity enrichment and high-resolution liquid chromatography with tandem mass spectrometry to profile the alterations of the acetylome in cat small intestine 10 days after infection by T. gondii Prugniuad (Pru) strain. Our analysis showed that T. gondii induced significant changes in the acetylation of proteins in the cat intestine. We identified 2606 unique lysine acetylation sites in 1357 acetylated proteins. The levels of 334 acetylated peptides were downregulated, while the levels of 82 acetylated peptides were increased in the infected small intestine. The proteins with differentially acetylated peptides were particularly enriched in the bioenergetics-related processes, such as tricarboxylic acid cycle, oxidative phosphorylation, and oxidation-reduction. These results provide the first baseline of the global acetylome of feline small intestine following T. gondii infection and should facilitate further analysis of the role of acetylated protein in the pathogenesis of T. gondii infection in its definitive host.

Citation

Meng, Y.-M., Zhai, B.-T., Elsheikha, H. M., Xie, S.-C., Wang, Z.-X., Zhao, Q., Zhu, X.-Q., & He, J.-J. (2020). Acetylome analysis of the feline small intestine following Toxoplasma gondii infection. Parasitology Research, 119(11), 3649-3657. https://doi.org/10.1007/s00436-020-06880-4

• Journal Article Type: Article
• Acceptance Date: Sep 7, 2020
• Online Publication Date: Sep 20, 2020
• Publication Date: Nov 1, 2020
• Deposit Date: Oct 23, 2020
• Publicly Available Date: Sep 21, 2021
• Journal: Parasitology Research
• Print ISSN: 0932-0113
• Electronic ISSN: 1432-1955
• Publisher: Springer Verlag
• Peer Reviewed: Peer Reviewed
• Volume: 119
• Issue: 11
• Pages: 3649-3657
• DOI: https://doi.org/10.1007/s00436-020-06880-4
• Keywords: Insect Science; General Veterinary; Parasitology; Infectious Diseases; General Medicine
• Public URL: https://nottingham-repository.worktribe.com/output/4985747
• Publisher URL: https://link.springer.com/article/10.1007/s00436-020-06880-4
• Additional Information: Meng, Y., Zhai, B., Elsheikha, H.M. et al. Acetylome analysis of the feline small intestine following Toxoplasma gondii infection. Parasitol Res 119, 3649–3657 (2020). https://doi.org/10.1007/s00436-020-06880-4