Complete genome sequence and comparative analysis of two potential probiotics Bacillus subtilis isolated from honey and honeybee microbiomes

Hamdy, Abdelhamid A.; Esawy, Mona A.; Elattal, Nouran A.; Amin, Magdy A.; Ali, Amal E.; Awad, Ghada E. A.; Connerton, Ian; Mansour, Nahla M.

Abstract

Background: We have previously isolated Bacillus subtilis HMNig-2 and MENO2 strains, from honey and the honeybee gut microbiome respectively, and demonstrated these strains to produce levansucrase with potential probiotics characteristics. Here we report their complete genome sequence and comparative analysis with other and other B. subtilis strains. Results: The complete genome sequences of Bacillus subtilis HMNig-2 and MENO2 were de novo assembled from MiSeq paired-end sequence reads and annotated using the RAST tool. Whole-genome alignments were performed to identify functional differences associated with their potential use as probiotics. Conclusions: The comparative analysis and the availability of the genome sequence of these two strains will provide comprehensive analysis about the diversity of these valuable Bacillus strains and the possible impact of the environment on bacterial evolution. Significance and impact of study: We introduce complete genome sequence of two new B. subtilis strains HMNig-2 and MENO2 with probiotic potential and as cell factories for the production of levan and other valuable components for pharmaceutical and industrial applications

Citation

Hamdy, A. A., Esawy, M. A., Elattal, N. A., Amin, M. A., Ali, A. E., Awad, G. E. A., Connerton, I., & Mansour, N. M. (2020). Complete genome sequence and comparative analysis of two potential probiotics Bacillus subtilis isolated from honey and honeybee microbiomes. Journal of Genetic Engineering and Biotechnology, 18(1), Article 34. https://doi.org/10.1186/s43141-020-00050-w

• Journal Article Type: Article
• Acceptance Date: Jul 8, 2020
• Online Publication Date: Jul 22, 2020
• Publication Date: 2020-12
• Deposit Date: Apr 25, 2025
• Publicly Available Date: Apr 25, 2025
• Journal: Journal of Genetic Engineering and Biotechnology
• Print ISSN: 1687-157X
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 18
• Issue: 1
• Article Number: 34
• DOI: https://doi.org/10.1186/s43141-020-00050-w
• Keywords: Biotechnology; Genetics
• Public URL: https://nottingham-repository.worktribe.com/output/4784240
• Publisher URL: https://www.sciencedirect.com/science/article/pii/S1687157X23005656?via%3Dihub