Bianca Carla Silva Campitelli Barros
Secreted factors of Aspergillus fumigatus cause lung epithelial barrier disruption: A study using an air–liquid interface cell culture model
Barros, Bianca Carla Silva Campitelli; Barros, Debora Tereza Lucas; Brock, Matthias; Toledo, Marcos Sergio; Serrano, Solange Maria Toledo; Suzuki, Erika; Ghaemmaghami, Amir M
Authors
Debora Tereza Lucas Barros
Dr MATTHIAS BROCK Matthias.Brock@nottingham.ac.uk
ASSOCIATE PROFESSOR
Marcos Sergio Toledo
Solange Maria Toledo Serrano
Erika Suzuki
Professor AMIR GHAEMMAGHAMI AMIR.GHAEMMAGHAMI@NOTTINGHAM.AC.UK
PROFESSOR OF IMMUNOLOGY AND IMMUNO- BIOENGINEERING
Abstract
The effects of Aspergillus fumigatus-conditioned medium (AFCM) on the integrity of the Calu-3 cell lung epithelial barrier were investigated. AFCM led to a decrease in transepithelial electrical resistance and the disruption of the occludin network in the epithelial barrier. Preincubation with protease inhibitors reduced the effect of AFCM by ~ 90%, demonstrating the role of fungal proteases in epithelial barrier disruption. By mass spectrometry, we identified 494 unique proteins in AFCM, including 14 peptidases of different families. Together, these findings suggest that proteases secreted by A. fumigatus were able to modulate host epithelial barrier disruption in this fungal infection process.
Citation
Barros, B. C. S. C., Barros, D. T. L., Brock, M., Toledo, M. S., Serrano, S. M. T., Suzuki, E., & Ghaemmaghami, A. M. (2025). Secreted factors of Aspergillus fumigatus cause lung epithelial barrier disruption: A study using an air–liquid interface cell culture model. Medical Mycology, 63(3), Article myaf018. https://doi.org/10.1093/mmy/myaf018
| Journal Article Type | Article |
|---|---|
| Acceptance Date | Feb 25, 2025 |
| Online Publication Date | Feb 26, 2025 |
| Publication Date | 2025-03 |
| Deposit Date | Mar 13, 2025 |
| Publicly Available Date | Feb 27, 2026 |
| Journal | Medical Mycology |
| Print ISSN | 1369-3786 |
| Electronic ISSN | 1460-2709 |
| Publisher | Oxford University Press |
| Peer Reviewed | Peer Reviewed |
| Volume | 63 |
| Issue | 3 |
| Article Number | myaf018 |
| DOI | https://doi.org/10.1093/mmy/myaf018 |
| Keywords | Aspergillus fumigatus, epithelial barrier, air–liquid interface, disruption, protease |
| Public URL | https://nottingham-repository.worktribe.com/output/46466853 |
| Publisher URL | https://academic.oup.com/mmy/article/63/3/myaf018/8043262 |
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