Enhancing nanopore adaptive sampling for PromethION using readfish at scale

Munro, Rory; Payne, Alex; Holmes, Nadine; Moore, Chris; Cahyani, Inswasti; Loose, Matt

Abstract

A unique feature of Oxford Nanopore Technologies sequencers, adaptive sampling, allows precise DNA molecule selection from sequencing libraries. Here we present enhancements to our tool, readfish, enabling all features for the industrial scale PromethION sequencer, including standard and "barcode-aware" adaptive sampling. We demonstrate effective coverage enrichment and assessment of multiple human genomes for copy number and structural variation on a single PromethION flow cell.

Citation

Munro, R., Payne, A., Holmes, N., Moore, C., Cahyani, I., & Loose, M. (2025). Enhancing nanopore adaptive sampling for PromethION using readfish at scale. Genome Research, https://doi.org/10.1101/gr.279329.124

• Journal Article Type: Article
• Acceptance Date: Jan 15, 2025
• Online Publication Date: Jan 30, 2025
• Publication Date: Jan 30, 2025
• Deposit Date: Feb 4, 2025
• Publicly Available Date: Feb 4, 2025
• Journal: Genome Research
• Print ISSN: 1088-9051
• Electronic ISSN: 1549-5469
• Publisher: Cold Spring Harbor Laboratory Press
• Peer Reviewed: Peer Reviewed
• DOI: https://doi.org/10.1101/gr.279329.124
• Public URL: https://nottingham-repository.worktribe.com/output/44826076
• Publisher URL: https://genome.cshlp.org/content/early/2025/01/30/gr.279329.124