Lidia Delgado
Performance of the extremophilic enzyme BglA in the hydrolysis of two aroma glucosides in a range of model and real wines and juices
Delgado, Lidia; Parker, Mango; Fisk, Ian; Paradisi, Francesca
Authors
Mango Parker
Professor IAN FISK IAN.FISK@NOTTINGHAM.AC.UK
Professor of Flavour Science
Francesca Paradisi
Abstract
β-Glycosidases enhance wine aroma by releasing volatile aglycones from non-volatile glycosides. Commercial preparations contain primarily pectinases, with β-glycosidase as a secondary activity, which limits their potential. Here, the extremophilic β-glucosidase A from Halothermothix orenii, (BglA) has been compared with Rapidase® for the production of aromatic wines and in the remediation of smoke-tainted wines. Model systems, real juices and wines have been enriched with geranyl glucoside, typical of white varieties, and guaiacyl glucoside, commonly found in red wines exposed to oak and wines made from grapes exposed to smoke. The hydrolytic capacity of BglA was evaluated by measuring the released volatiles in the gas phase with solid-phase microextraction and GC–MS. BglA, despite an apparent instability at low pH, is twice as effective in releasing volatiles in sweeter wines and in grape juices, offering an excellent alternative for the early stages of the winemaking process and in the juice industry.
Citation
Delgado, L., Parker, M., Fisk, I., & Paradisi, F. (2020). Performance of the extremophilic enzyme BglA in the hydrolysis of two aroma glucosides in a range of model and real wines and juices. Food Chemistry, 323, Article 126825. https://doi.org/10.1016/j.foodchem.2020.126825
Journal Article Type | Article |
---|---|
Acceptance Date | Apr 13, 2020 |
Online Publication Date | Apr 15, 2020 |
Publication Date | Sep 1, 2020 |
Deposit Date | Mar 29, 2021 |
Publicly Available Date | Aug 13, 2021 |
Journal | Food Chemistry |
Print ISSN | 0308-8146 |
Electronic ISSN | 1873-7072 |
Publisher | Elsevier |
Peer Reviewed | Peer Reviewed |
Volume | 323 |
Article Number | 126825 |
DOI | https://doi.org/10.1016/j.foodchem.2020.126825 |
Keywords | Food Science; Analytical Chemistry; General Medicine |
Public URL | https://nottingham-repository.worktribe.com/output/4309616 |
Publisher URL | https://www.sciencedirect.com/science/article/abs/pii/S0308814620306877 |
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Performance Of The Extremophilic Enzyme BglA In The Hydrolysis Of Two Aroma Glucosides In A Range Of Model And Real Wines And Juices
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