The Effect of Whey Protein Supplementation on Myofibrillar Protein Synthesis and Performance Recovery in Resistance-Trained Men

Davies, Robert W.; Bass, Joseph J.; Carson, Brian P.; Norton, Catherine; Kozior, Marta; Wilkinson, Daniel J.; Brook, Matthew S.; Atherton, Philip J.; Smith, Ken; Jakeman, Philip M.

Abstract

Background: The aim of this study was to investigate the effect of whey protein supplementation on myofibrillar protein synthesis (myoPS) and muscle recovery over a 7-d period of intensified resistance training (RT). Methods: In a double-blind randomised parallel group design, 16 resistance-trained men aged 18 to 35 years completed a 7-d RT protocol, consisting of three lower-body RT sessions on non-consecutive days. Participants consumed a controlled diet (146 kJ·kg−1·d−1, 1.7 g·kg−1·d−1 protein) with either a whey protein supplement or an isonitrogenous control (0.33 g·kg−1·d−1 protein). To measure myoPS, 400 ml of deuterium oxide (D2O) (70 atom %) was ingested the day prior to starting the study and m. vastus lateralis biopsies were taken before and after RT-intervention. Myofibrillar fractional synthetic rate (myoFSR) was calculated via deuterium labelling of myofibrillar-bound alanine, measured by gas chromatography-pyrolysis-isotope ratio mass spectrometry (GC-Pyr-IRMS). Muscle recovery parameters (i.e., countermovement jump height, isometric-squat force, muscle soreness and serum creatine kinase) were assessed daily. Results: MyoFSR PRE was 1.6 (0.2) %∙d−1 (mean (SD)). Whey protein supplementation had no effect on myoFSR (p = 0.771) or any recovery parameter (p = 0.390–0.989). Conclusions: Over an intense 7-d RT protocol, 0.33 g·kg−1·d−1 of supplemental whey protein does not enhance day-to-day measures of myoPS or postexercise recovery in resistance-trained men.

Citation

Davies, R. W., Bass, J. J., Carson, B. P., Norton, C., Kozior, M., Wilkinson, D. J., Brook, M. S., Atherton, P. J., Smith, K., & Jakeman, P. M. (2020). The Effect of Whey Protein Supplementation on Myofibrillar Protein Synthesis and Performance Recovery in Resistance-Trained Men. Nutrients, 12(3), Article 845. https://doi.org/10.3390/nu12030845

• Journal Article Type: Article
• Acceptance Date: Mar 20, 2020
• Online Publication Date: Mar 21, 2020
• Publication Date: Mar 21, 2020
• Deposit Date: Mar 27, 2020
• Publicly Available Date: Mar 30, 2020
• Journal: Nutrients
• Electronic ISSN: 2072-6643
• Publisher: MDPI
• Peer Reviewed: Peer Reviewed
• Volume: 12
• Issue: 3
• Article Number: 845
• DOI: https://doi.org/10.3390/nu12030845
• Public URL: https://nottingham-repository.worktribe.com/output/4211829
• Publisher URL: https://www.mdpi.com/2072-6643/12/3/845