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Bacteriophages to Control Campylobacter in Commercially Farmed Broiler Chickens, in Australia

Chinivasagam, Helene N.; Estella, Wiyada; Maddock, Lance; Mayer, David G.; Weyand, Caitlin; Connerton, Phillippa L.; Connerton, Ian F.

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Authors

Helene N. Chinivasagam

Wiyada Estella

Lance Maddock

David G. Mayer

Caitlin Weyand

Phillippa L. Connerton

IAN CONNERTON IAN.CONNERTON@NOTTINGHAM.AC.UK
Northern Foods Professor of Food Safety



Abstract

© Copyright © 2020 The State of Queensland (through the Department Agriculture and Fisheries). This study describes the development and use of bacteriophage cocktails to control Campylobacter in broiler chickens, in a commercial setting, in Queensland Australia, following the birds from farm to the processing plant. The components of the bacteriophage cocktails were selected to be effective against the maximum number of Campylobacter jejuni and Campylobacter coli isolates encountered on SE Queensland farms. Farms were identified that had suitable Campylobacter target populations and phage were undetectable 1 week prior to the intended treatment. Cocktails of phages were administered at 47 days of age. Groups of study birds were slaughtered the following day, on-farm, at the end of flock transport to the plant, and at processing (approximately 28 h post-treatment). On Farm A, the phage treatment significantly reduced Campylobacter levels in the ceca at the farm in the range of 1–3 log10 CFU/g (p = 0.007), compared to mock treated controls. However, individual birds sampled on farm (1/10) or following transport (2/10) exhibited high cecal Campylobacter counts with low phage titers, suggesting that treatment periods > 24 h may be required to ensure phage replication for effective biocontrol in vivo. At the time of the trial the control birds in Farm B were phage positive despite having been negative one week earlier. There was no significant difference in the cecal Campylobacter counts between the treatment and control groups following treatment but a fall of 1.7 log10 CFU/g was observed from that determined from birds collected the previous week (p = 0.0004). Campylobacter isolates from both farms retained sensitivity to the treatment phages. These trials demonstrated bacteriophages sourced from Queensland farms have the potential to reduce intestinal Campylobacter levels in market ready broiler chickens.

Citation

Chinivasagam, H. N., Estella, W., Maddock, L., Mayer, D. G., Weyand, C., Connerton, P. L., & Connerton, I. F. (2020). Bacteriophages to Control Campylobacter in Commercially Farmed Broiler Chickens, in Australia. Frontiers in Microbiology, 11, https://doi.org/10.3389/fmicb.2020.00632

Journal Article Type Article
Acceptance Date Mar 20, 2020
Online Publication Date Apr 27, 2020
Publication Date Apr 27, 2020
Deposit Date Mar 27, 2020
Publicly Available Date Mar 28, 2024
Journal Frontiers in Microbiology
Electronic ISSN 1664-302X
Publisher Frontiers Media
Peer Reviewed Peer Reviewed
Volume 11
Article Number 632
DOI https://doi.org/10.3389/fmicb.2020.00632
Public URL https://nottingham-repository.worktribe.com/output/4210699
Publisher URL https://www.frontiersin.org/articles/10.3389/fmicb.2020.00632/full

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