Dr Eleanor Bellows Eleanor.Bellows@nottingham.ac.uk
Research Fellow
The expanding role of cap-adjacent modifications in animals
Bellows, Eleanor; Fray, Rupert G.; Knight, Helen M.; Archer, Nathan
Authors
Professor RUPERT FRAY RUPERT.FRAY@NOTTINGHAM.AC.UK
PROFESSOR OF EPITRANSCRIPTOMICS
Dr HELEN KNIGHT HELEN.KNIGHT@NOTTINGHAM.AC.UK
ASSOCIATE PROFESSOR
Dr NATHAN ARCHER Nathan.Archer@nottingham.ac.uk
ASSISTANT PROFESSOR
Abstract
Eukaryotic mRNA cap structures directly influence mRNA stability, translation, and immune recognition. While the significance of the mRNA cap itself has been well-established, recent research has revealed the intricate modifications to the nucleotides immediately adjacent to the cap. These include 2′-O-methylation (Cap1, Cap2) and N6-methyladenosine (m6Am), which recent work suggests may be dynamically regulated, interdependent, and vital for gene-regulation. This perspective explores the expanding role of cap-adjacent modifications. Cap1 is found on metazoan mRNA, viruses which infect metazoans, and the COVID-19 mRNA vaccines. We combined basic local alignment and 2D-TLC to track cap-adjacent modifying proteins and the modifications themselves to profile their prevalence throughout eukaryotes. We confirm that Cap1 is broadly metazoan specific, but surprisingly also in a brown algae. Additionally, we find genomic presence of the eraser, FTO more predictive of the occurrence of m6Am than the m6Am-writer, PCIF1. We then outline future directions to understand and exploit these enigmatic modifications.
Citation
Bellows, E., Fray, R. G., Knight, H. M., & Archer, N. (2024). The expanding role of cap-adjacent modifications in animals. Frontiers in RNA Research, 2, Article 1485307. https://doi.org/10.3389/frnar.2024.1485307
| Journal Article Type | Article |
|---|---|
| Acceptance Date | Sep 18, 2024 |
| Online Publication Date | Oct 1, 2024 |
| Publication Date | Oct 1, 2024 |
| Deposit Date | Oct 8, 2024 |
| Publicly Available Date | Oct 1, 2024 |
| Journal | Frontiers in RNA Research |
| Electronic ISSN | 2813-7116 |
| Publisher | Frontiers Media |
| Peer Reviewed | Peer Reviewed |
| Volume | 2 |
| Article Number | 1485307 |
| DOI | https://doi.org/10.3389/frnar.2024.1485307 |
| Keywords | mRNA cap, mRNA, epicapome, epitranscriptome, gene expression, translation |
| Public URL | https://nottingham-repository.worktribe.com/output/40552642 |
| Publisher URL | https://www.frontiersin.org/journals/rna-research/articles/10.3389/frnar.2024.1485307/full |
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Publisher Licence URL
https://creativecommons.org/licenses/by/4.0/
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