Auxin-dependent control of a plasmodesmal regulator creates a negative feedback loop modulating lateral root emergence

Sager, Ross; Wang, Xu; Hill, Kristine; Yoo, Byung Chun; Caplan, Jeffery; Nedo, Alex; Tran, Thu; Bennett, Malcolm J.; Lee, Jung Youn

Abstract

© 2020, The Author(s). Lateral roots originate from initial cells deep within the main root and must emerge through several overlying layers. Lateral root emergence requires the outgrowth of the new primordium (LRP) to coincide with the timely separation of overlying root cells, a developmental program coordinated by the hormone auxin. Here, we report that in Arabidopsis thaliana roots, auxin controls the spatiotemporal expression of the plasmodesmal regulator PDLP5 in cells overlying LRP, creating a negative feedback loop. PDLP5, which functions to restrict the cell-to-cell movement of signals via plasmodesmata, is induced by auxin in cells overlying LRP in a progressive manner. PDLP5 localizes to plasmodesmata in these cells and negatively impacts organ emergence as well as overall root branching. We present a model, incorporating the spatiotemporal expression of PDLP5 in LRP-overlying cells into known auxin-regulated LRP-overlying cell separation pathways, and speculate how PDLP5 may function to negatively regulate the lateral root emergence process.

Citation

Sager, R., Wang, X., Hill, K., Yoo, B. C., Caplan, J., Nedo, A., Tran, T., Bennett, M. J., & Lee, J. Y. (2020). Auxin-dependent control of a plasmodesmal regulator creates a negative feedback loop modulating lateral root emergence. Nature Communications, 11(1), Article 364. https://doi.org/10.1038/s41467-019-14226-7

• Journal Article Type: Article
• Acceptance Date: Dec 16, 2019
• Online Publication Date: Jan 17, 2020
• Publication Date: Jan 17, 2020
• Deposit Date: Feb 24, 2020
• Publicly Available Date: Feb 24, 2020
• Journal: Nature Communications
• Electronic ISSN: 2041-1723
• Publisher: Nature Publishing Group
• Peer Reviewed: Peer Reviewed
• Volume: 11
• Issue: 1
• Article Number: 364
• DOI: https://doi.org/10.1038/s41467-019-14226-7
• Public URL: https://nottingham-repository.worktribe.com/output/3753087
• Publisher URL: https://www.nature.com/articles/s41467-019-14226-7#Abs1
• Additional Information: Received: 5 June 2019; Accepted: 16 December 2019; First Online: 17 January 2020; : The authors declare no competing interests.