Microfluidic-based Growth and Imaging of Bacterial Biofilms

Brown, James; Blunk, Birte; Williams, Paul; Hardie, Kim

Abstract

Biofilms are a ubiquitous form of growth for bacteria on surfaces in most environments, natural or manmade. Here we present a protocol using the Bioflux microfluidic system to investigate the organized structure and development of these multicellular communities. Microfluidic systems present an opportunity to grow biofilms in a stable, physiologically maintained environment that is readily observable via time-lapse microscopy.

Citation

Brown, J., Blunk, B., Williams, P., & Hardie, K. (2019). Microfluidic-based Growth and Imaging of Bacterial Biofilms. Bio-protocol, 9(24), Article e3460. https://doi.org/10.21769/BioProtoc.3460

• Journal Article Type: Article
• Acceptance Date: Nov 14, 2019
• Online Publication Date: Dec 20, 2019
• Publication Date: Dec 20, 2019
• Deposit Date: Jan 30, 2020
• Publicly Available Date: Jan 30, 2020
• Journal: Bio-protocol
• Publisher: Bio-Protocol
• Peer Reviewed: Peer Reviewed
• Volume: 9
• Issue: 24
• Article Number: e3460
• DOI: https://doi.org/10.21769/BioProtoc.3460
• Public URL: https://nottingham-repository.worktribe.com/output/3735185
• Publisher URL: https://bio-protocol.org/bio101/e3460
• Additional Information: Copyright © 2019 The Authors; exclusive licensee Bio-protocol LLC