Cell Death in Cells Overlying Lateral Root Primordia Facilitates Organ Growth in Arabidopsis

Escamez, Sacha; Andr�, Domenique; Sztojka, Bernadette; Bollh�ner, Benjamin; Hall, Hardy; Berthet, B�atrice; Vo�, Ute; Lers, Amnon; Maizel, Alexis; Andersson, Magnus; Bennett, Malcolm; Tuominen, Hannele

Abstract

© 2019 The Authors Plant organ growth is widely accepted to be determined by cell division and cell expansion, but, unlike that in animals, the contribution of cell elimination has rarely been recognized. We investigated this paradigm during Arabidopsis lateral root formation, when the lateral root primordia (LRP) must traverse three overlying cell layers within the parent root. A subset of LRP-overlying cells displayed the induction of marker genes for cell types undergoing developmental cell death, and their cell death was detected by electron, confocal, and light sheet microscopy techniques. LRP growth was delayed in cell-death-deficient mutants lacking the positive cell death regulator ORESARA1/ANAC092 (ORE1). LRP growth was restored in ore1-2 knockout plants by genetically inducing cell elimination in cells overlying the LRP or by physically killing LRP-overlying cells by ablation with optical tweezers. Our results support that, in addition to previously discovered mechanisms, cell elimination contributes to regulating lateral root emergence. Escamez et al. report that a subset of cells overlying newly formed lateral roots within the parent root dies to facilitate early lateral root organ growth. Our findings suggest that, contrary to common belief, cell death can, as in animals, regulate organ growth in plants, which may have implications for our understanding of evolution.

Citation

Escamez, S., André, D., Sztojka, B., Bollhöner, B., Hall, H., Berthet, B., Voß, U., Lers, A., Maizel, A., Andersson, M., Bennett, M., & Tuominen, H. (2020). Cell Death in Cells Overlying Lateral Root Primordia Facilitates Organ Growth in Arabidopsis. Current Biology, 30(3), 455-464.e7. https://doi.org/10.1016/j.cub.2019.11.078

• Journal Article Type: Article
• Acceptance Date: Nov 26, 2019
• Online Publication Date: Jan 16, 2020
• Publication Date: Feb 3, 2020
• Deposit Date: Dec 18, 2019
• Publicly Available Date: Jan 17, 2021
• Journal: Current Biology
• Print ISSN: 0960-9822
• Electronic ISSN: 1879-0445
• Publisher: Cell Press
• Peer Reviewed: Peer Reviewed
• Volume: 30
• Issue: 3
• Pages: 455-464.e7
• DOI: https://doi.org/10.1016/j.cub.2019.11.078
• Keywords: General Biochemistry, Genetics and Molecular Biology; General Agricultural and Biological Sciences
• Public URL: https://nottingham-repository.worktribe.com/output/3594160
• Publisher URL: https://www.sciencedirect.com/science/article/pii/S0960982219315805
• Additional Information: This article is maintained by: Elsevier; Article Title: Cell Death in Cells Overlying Lateral Root Primordia Facilitates Organ Growth in Arabidopsis; Journal Title: Current Biology; CrossRef DOI link to publisher maintained version: https://doi.org/10.1016/j.cub.2019.11.078; Content Type: article; Copyright: © 2019 The Authors. Published by Elsevier Ltd.