PIMMS-Dash: Accessible analysis, interrogation, and visualisation of high-throughput transposon insertion sequencing (TIS) data

Blanchard, Adam M.; Taylor, Adam; Warry, Andrew; Shephard, Freya; Curwen, Alice; Leigh, James A.; Emes, Richard D.; Egan, Sharon A.

Abstract

Motivation Current methods for visualising and interrogating high-throughput transposon insertion mutagenesis sequencing (TIS) data requires a significant time investment in learning bioinformatics, often producing static figures that do not facilitate real time analysis.

Summary We have created an accessible web-based browser tool for visualisation and downstream analysis of high-throughput TIS data. This includes multiple interactive and sortable tables to aid the user to identify genes of interest, enabling the user to gain a greater understanding of the genes contributing to fitness in their experimental work. PIMMS-Dash permits researchers, with any level of bioinformatics knowledge, to interrogate data sets and generate publication quality figures.

Availability PIMMS-Dash is freely available and is accessible online at https://pimms-dashboard-uon.azurewebsites.net

Citation

Blanchard, A. M., Taylor, A., Warry, A., Shephard, F., Curwen, A., Leigh, J. A., Emes, R. D., & Egan, S. A. (2024). PIMMS-Dash: Accessible analysis, interrogation, and visualisation of high-throughput transposon insertion sequencing (TIS) data

• Working Paper Type: Preprint
• Online Publication Date: Apr 14, 2024
• Publication Date: Apr 14, 2024
• Deposit Date: Jan 2, 2025
• Publicly Available Date: Jan 7, 2025
• DOI: https://doi.org/10.1101/2024.04.10.588854
• Public URL: https://nottingham-repository.worktribe.com/output/33839018
• Publisher URL: https://www.biorxiv.org/content/10.1101/2024.04.10.588854v1