Broccoli and watercress suppress matrix metalloproteinase-9 activity and invasiveness of human MDA-MB-231 breast cancer cells

Rose, Peter; Huang, Qing; Ong, Choon Nam; Whiteman, Matt

Abstract

A high dietary intake of cruciferous vegetables has been associated with a reduction in numerous human pathologies particularly cancer. In the current study, we examined the inhibitory effects of broccoli (Brassica oleracea var. italica) and watercress (Rorripa nasturtium aquaticum) extracts on 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced cancer cell invasion and matrix metalloproteinase-9 activity using human MDA-MB-231 breast cancer cells. Aberrant overexpression of matrix metalloproteinases, including metalloproteinase-9, is associated with increased invasive potential in cancer cell lines. Our results demonstrate that extracts of broccoli and Rorripa suppressed TPA-induced MMP-9 activity and invasiveness in a concentration dependant manner as determined by zymographic analysis. Furthermore, fractionation of individual extracts followed by liquid chromatography mass spectroscopy analysis (LC-MS) revealed that the inhibitory effects of each vegetable were associated with the presence of 4-methysulfinylbutyl (sulforaphane) and 7-methylsulphinylheptyl isothiocyanates. Taken together, our data indicate that isothiocyanates derived form broccoli and Rorripa inhibit metalloproteinase 9 activities and also suppress the invasive potential of human MDA-MB-231 breast cancer cells in vitro. The inhibitory effects observed in the current study may contribute to the suppression of carcinogenesis by diets high in cruciferous vegetables. © 2005 Elsevier Inc. All rights reserved.

Citation

Rose, P., Huang, Q., Ong, C. N., & Whiteman, M. (2005). Broccoli and watercress suppress matrix metalloproteinase-9 activity and invasiveness of human MDA-MB-231 breast cancer cells. Toxicology and Applied Pharmacology, 209(2), 105-113. https://doi.org/10.1016/j.taap.2005.04.010

• Journal Article Type: Article
• Acceptance Date: Apr 6, 2005
• Online Publication Date: Jun 13, 2005
• Publication Date: Dec 1, 2005
• Deposit Date: Jan 29, 2025
• Journal: Toxicology and Applied Pharmacology
• Print ISSN: 0041-008X
• Electronic ISSN: 1096-0333
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 209
• Issue: 2
• Pages: 105-113
• DOI: https://doi.org/10.1016/j.taap.2005.04.010
• Keywords: Cruciferous vegetables, Isothiocyanate, Matrix metalloproteinase, Invasion, Chemoprevention
• Public URL: https://nottingham-repository.worktribe.com/output/3219668
• Publisher URL: https://www.sciencedirect.com/science/article/pii/S0041008X05001675?via%3Dihub#fig3