Dr DANIEL MCWILLIAMS DAN.MCWILLIAMS@NOTTINGHAM.AC.UK
Postdoctoral Research Fellow
Coexpression of gastrin and gastrin receptors (CCK-B and ΔCCK-B) in gastrointestinal tumour cell lines
McWilliams, D. F.; Watson, S. A.; Crosbee, D. M.; Michaeli, D.; Seth, R.
Authors
S. A. Watson
D. M. Crosbee
D. Michaeli
R. Seth
Abstract
Background - The peptide hormone gastrin is a recognised growth factor for gastrointestinal (GI) tumour cells. Carboxyamidated gastrins bind to the cell surface gastrin/cholecystokinin B (CCK-B) receptor which can be expressed as either a normal or a truncated isoform (ΔCCK-B). Aims - To compare gastrin gene expression with ΔCCK-B and total CCK-B (both isoforms) gene expression in both GI and non-GI tract derived human tumour cell lines. Methods - Total RNA was extracted and gene expression was assayed by the reverse transcription-polymerase chain reaction followed by Southern blotting and hybridisation with specific oligo probes. Results - Gastrin was expressed by 5/5 gastric and 7/8 colorectal cell lines. Coexpression of gastrin CCK-B isoform was found in 80% of gastric and 75% of colorectal cell lines. Non-GI cell lines, with the exception of a lymphoblastic leukaemia cell line, showed no coexpression. The truncated receptor, ΔCCK-B, was shown in 3/5 gastric and 5/8 colorectal cell lines and was always coexpressed with gastrin. Conclusion - The truncated gastrin receptor, ΔCCK-B, is coexpressed with gastrin in 8/13 GI tumour cell lines. Gastrin and CCK-B receptor isoforms may be involved in maintaining autocrine/paracrine growth pathways in GI cancer cells.
Citation
McWilliams, D. F., Watson, S. A., Crosbee, D. M., Michaeli, D., & Seth, R. (1998). Coexpression of gastrin and gastrin receptors (CCK-B and ΔCCK-B) in gastrointestinal tumour cell lines. Gut, 42(6), 795-798. https://doi.org/10.1136/gut.42.6.795
Journal Article Type | Article |
---|---|
Acceptance Date | Jan 19, 1998 |
Online Publication Date | Jun 1, 1998 |
Publication Date | Jun 1, 1998 |
Deposit Date | Jul 31, 2023 |
Journal | Gut |
Print ISSN | 0017-5749 |
Electronic ISSN | 1468-3288 |
Publisher | BMJ Publishing Group |
Peer Reviewed | Peer Reviewed |
Volume | 42 |
Issue | 6 |
Pages | 795-798 |
DOI | https://doi.org/10.1136/gut.42.6.795 |
Public URL | https://nottingham-repository.worktribe.com/output/3204314 |
Publisher URL | https://gut.bmj.com/content/42/6/795 |
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