Dermal absorption of high molecular weight parent and alkylated polycyclic aromatic hydrocarbons from manufactured gas plant soils using in vitro assessment

Williams-Clayson, Alison M; Vane, Christopher H; Jones, Matthew D; Thomas, Russell; Taylor, Christopher; Beriro, Darren J

Abstract

An enhanced in vitro human dermal bioavailability method was developed to measure the release of twenty parent and seven alkylated high molecular weight (HMW) polycyclic aromatic hydrocarbons (PAHs) from contaminated soils collected from five former manufactured Gas Plants (MGP) in England. GC-MS/MS was used to quantify HMW PAHs in soil, Strat-M artificial membrane representing skin, and synthetic receptor solution (RS) representing systemic circulation at 1-h, 10-h, and 24-h timesteps. Fluoranthene and pyrene exhibited the highest fluxes from soils to membrane (ranging from 9.5 - 281 ng/cm2/h) and soil to RS (<LOQ to 16.9 ng/cm2/h). Chrysene, benzo[a]anthracene, benzo[b]fluoranthene and the alkylated C1-fluoranthene/pyrene homologue series demonstrated fluxes higher than other HMW PAHs. The dermal fluxes were generally lower than those reported in previous investigations and suggests that dermal absorption varies between both HMW parent and alkylated PAHs and individual PAHs. The utilisation of real-world contaminated soils allowed for a more realistic representation; this is important because current risk assessment guidance is baseed on results from experiments that used artificially spiked soils. This research shows that the the ranges of dermal fluxes are PAH dependent and impact the mass of absorbed from soil after dermal exposure and therefore the potential risk contaminated soil poses to human health.

Citation

Williams-Clayson, A. M., Vane, C. H., Jones, M. D., Thomas, R., Taylor, C., & Beriro, D. J. (2024). Dermal absorption of high molecular weight parent and alkylated polycyclic aromatic hydrocarbons from manufactured gas plant soils using in vitro assessment. Journal of Hazardous Materials, 469, Article 133858. https://doi.org/10.1016/j.jhazmat.2024.133858

• Journal Article Type: Article
• Acceptance Date: Feb 20, 2024
• Online Publication Date: Feb 22, 2024
• Publication Date: May 5, 2024
• Deposit Date: Feb 29, 2024
• Publicly Available Date: Feb 23, 2025
• Journal: Journal of Hazardous Materials
• Print ISSN: 0304-3894
• Electronic ISSN: 1873-3336
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 469
• Article Number: 133858
• DOI: https://doi.org/10.1016/j.jhazmat.2024.133858
• Keywords: Bioavailability, Risk assessment, PAH, Percutaneous
• Public URL: https://nottingham-repository.worktribe.com/output/31893609
• Publisher URL: https://www.sciencedirect.com/science/article/pii/S0304389424004370?via%3Dihub