Expression of calpastatin isoforms in muscle and functionality of multiple calpastatin promoters

Parr, Tim; Jewell, Kirsty K.; Sensky, Paul L.; Brameld, John M.; Bardsley, Ronald G.; Buttery, Peter J.

Abstract

Calpastatin is the specific endogenous inhibitor of calpain proteinase that is encoded by a single gene. Transient transfection assays in both a non-fusing skeletal muscle and non-muscle cell-line demonstrated that the putative porcine calpastatin promoter regions 5? to exons 1xa, 1xb, and 1u were functional. Both real-time quantitative and semi-quantitative RT-PCR on porcine skeletal muscle total RNA indicated that steady-state expression of Type I and III mRNAs containing exons 1xa and 1u, respectively, was at equivalent levels whilst the expression of Type II mRNA containing exon 1xb was significantly less (p

Citation

Parr, T., Jewell, K. K., Sensky, P. L., Brameld, J. M., Bardsley, R. G., & Buttery, P. J. (2004). Expression of calpastatin isoforms in muscle and functionality of multiple calpastatin promoters. Archives of Biochemistry and Biophysics, 427(1), 8-15. https://doi.org/10.1016/j.abb.2004.04.001

• Journal Article Type: Article
• Online Publication Date: May 6, 2004
• Publication Date: Jul 1, 2004
• Deposit Date: Apr 27, 2021
• Journal: Archives of Biochemistry and Biophysics
• Print ISSN: 0003-9861
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 427
• Issue: 1
• Pages: 8-15
• DOI: https://doi.org/10.1016/j.abb.2004.04.001
• Public URL: https://nottingham-repository.worktribe.com/output/3153912
• Publisher URL: https://www.sciencedirect.com/science/article/abs/pii/S0003986104002061?via%3Dihub