Enhanced uptake of nanoparticle drug carriers via a thermoresponsive shell enhances cytotoxicity in a cancer cell line

Abulateefeh, Samer R.; Spain, Sebastian G.; Thurecht, Kristofer J.; Aylott, Jonathan W.; Chan, Weng C.; Garnett, Martin C.; Alexander, Cameron

Abstract

Polymer particles consisting of a biodegradable poly[lactide-co-glycolide] (PLGA) core and a thermoresponsive shell have been formulated to encapsulate the dye rhodamine 6G and the potent cytotoxic drug paclitaxel. Cellular uptake of these particles is significantly enhanced above the thermal transition temperature (TTT) of the polymer shells in the human breast carcinoma cell line MCF-7 as determined by flow cytometry and fluorescence microscopy. Paclitaxel-loaded particles display reduced and enhanced cytotoxicity below and above the TTT respectively compared to unencapsulated drug. The data suggests a potential route to enhanced anti-cancer efficacy through temperature-mediated cell targeting.© The Royal Society of Chemistry 2013.

Citation

Abulateefeh, S. R., Spain, S. G., Thurecht, K. J., Aylott, J. W., Chan, W. C., Garnett, M. C., & Alexander, C. (2013). Enhanced uptake of nanoparticle drug carriers via a thermoresponsive shell enhances cytotoxicity in a cancer cell line. Biomaterials Science, 1(4), 434-442. https://doi.org/10.1039/c2bm00184e

• Journal Article Type: Article
• Acceptance Date: Jan 5, 2013
• Online Publication Date: Jan 14, 2013
• Publication Date: Apr 1, 2013
• Deposit Date: Sep 10, 2020
• Publicly Available Date: Sep 29, 2020
• Journal: Biomaterials Science
• Electronic ISSN: 2047-4849
• Publisher: Royal Society of Chemistry
• Peer Reviewed: Peer Reviewed
• Volume: 1
• Issue: 4
• Pages: 434-442
• DOI: https://doi.org/10.1039/c2bm00184e
• Public URL: https://nottingham-repository.worktribe.com/output/3137656
• Publisher URL: https://pubs.rsc.org/en/content/articlelanding/2013/BM/c2bm00184e#!divAbstract