In vitro assessment of the bioaccessibility of tocopherol and fatty acids from sunflower seed oil bodies

White, Daniel A.; Fisk, Ian D.; Makkhun, Sakunkhun; Gray, David A.

Abstract

The in vitro digestibility (proteolytic and lipolytic) and bioaccessibility of nutritionally important compounds (α-tocopherol and fatty acids) have been studied for natural sunflower (Helianthus annuus) oil body suspensions in comparison to artificial emulsions emulsified with polyoxyethylene-20-sorbitan- monolaurate (Tween 20) or whey protein isolate. Proteolytic digestion of emulsions with pepsin (pH 2) promoted significant increases in mean particle size of the whey protein isolate stabilized emulsion (1.8-2.9 μm) and oil bodies (2.3-22.5 μm) but not the Tween 20 stabilized emulsions. SDS-PAGE of proteolytic digestion products suggested degradation of the stabilizing oleosin protein (ca. 18-21 kDa) in oil bodies. The rate of oil body hydrolysis with lipase was significantly slower than the lipase-catalyzed hydrolysis of the artificial emulsions and exhibited a prolonged lag phase. Results from simulated human digestion in vitro suggested that the mean bioaccessibility of α-tocopherol and total fatty acids from oil bodies (0.6 and 8.4%, respectively) was significantly lower than that from the Tween 20 stabilized emulsion (35 and 52%, respectively) and the whey protein isolate stabilized emulsion (17 and 33%, respectively). These in vitro results suggest that oil bodies could provide a natural emulsion in food that is digested at a relatively slow rate, the physiological consequence of which may be increased satiety. © 2009 American Chemical Society.

Citation

White, D. A., Fisk, I. D., Makkhun, S., & Gray, D. A. (2009). In vitro assessment of the bioaccessibility of tocopherol and fatty acids from sunflower seed oil bodies. Journal of Agricultural and Food Chemistry, 57(13), 5720-5726. https://doi.org/10.1021/jf9003412

• Journal Article Type: Article
• Acceptance Date: Apr 8, 2009
• Online Publication Date: Jun 3, 2009
• Publication Date: Jul 8, 2009
• Deposit Date: Sep 6, 2021
• Journal: Journal of Agricultural and Food Chemistry
• Print ISSN: 0021-8561
• Electronic ISSN: 1520-5118
• Publisher: American Chemical Society
• Peer Reviewed: Peer Reviewed
• Volume: 57
• Issue: 13
• Pages: 5720-5726
• DOI: https://doi.org/10.1021/jf9003412
• Public URL: https://nottingham-repository.worktribe.com/output/3125831
• Publisher URL: https://pubs.acs.org/doi/10.1021/jf9003412