Imaging and Quantitative Methods for Studying Cytoskeletal Rearrangements During Root Development and Gravitropism

Jacques, Eveline; Wells, Darren M.; Bennett, Malcolm J.; Vissenberg, Kris

Abstract

High-resolution imaging of cytoskeletal structures paves the way for standardized methods to quantify cytoskeletal organization. Here we provide a detailed description of the analysis performed to determine the microtubule patterns in gravistimulated roots, using the recently developed software tool MicroFilament Analyzer.

Citation

Jacques, E., Wells, D. M., Bennett, M. J., & Vissenberg, K. (2015). Imaging and Quantitative Methods for Studying Cytoskeletal Rearrangements During Root Development and Gravitropism. In E. B. Blancaflor (Ed.), Plant Gravitropism; Methods and Protocols (81-89). Springer New York. https://doi.org/10.1007/978-1-4939-2697-8_8

• Publication Date: 2015-05
• Deposit Date: Jun 15, 2024
• Peer Reviewed: Peer Reviewed
• Pages: 81-89
• Series Title: Methods in Molecular Biology
• Series ISSN: 1064-3745
• Book Title: Plant Gravitropism; Methods and Protocols
• Chapter Number: 8
• ISBN: 9781493926961
• DOI: https://doi.org/10.1007/978-1-4939-2697-8_8
• Keywords: Microtubules , Gravistimulation , Quantification tool
• Public URL: https://nottingham-repository.worktribe.com/output/3092258
• Publisher URL: https://link.springer.com/protocol/10.1007/978-1-4939-2697-8_8