Modulation of T regulatory and dendritic cell phenotypes following ingestion of Bifidobacterium longum, active hexose correlated compound (AHCC®) and azithromycin in healthy individuals

Chowdhury, Abeed H; C�mara, Miguel; Verma, Chandan; Eremin, Oleg; Kulkarni, Anil D; Lobo, Dileep N

Abstract

The probiotic Bifidus BB536 (BB536), which contains Bifidobacterium longum, has been shown to have enhanced probiotic effects when given together with a standardized extract of cultured Lentinula edodes mycelia (AHCC®, Amino Up Co. Ltd., Sapporo, Japan). BB536 and AHCC® may modulate T cell and dendritic cell (DC) phenotypes, and cytokine profiles to favour anti-inflammatory responses following antibiotic ingestion. We tested the hypothesis that orally administered BB536 and/or AHCC®, results in modulation of immune effector cells with polarisation towards anti-inflammatory responses following antibiotic usage. Forty healthy male volunteers divided into 4 equal groups were randomised to receive either placebo, BB536, AHCC® or a combination for 12 days in a double-blind manner. After 7 days volunteers also received 250 mg azithromycin for 5 days. Cytokine profiles from purified CD3+ T cells stimulated with PDB-ionomycin were assessed. CD4+ CD25+ forkhead box P3 (Foxp3) expression and peripheral blood DC subsets were assessed prior to treatment and subsequently at 7 and 13 days. There was no difference in cytokine secretion from stimulated CD3+ T cells between treatment groups. Compared with baseline, Foxp3 expression (0.45 ± 0.1 vs. 1.3 ± 0.4; p = 0.002) and interferon-gamma/interleukin-4 (IFN-γ/IL-4) ratios were increased post-treatment in volunteers receiving BB536 (p = 0.031), although differences between groups were not significant. For volunteers receiving combination BB536 and AHCC®, there was an increase in myeloid dendritic cells (mDC) compared with plasmacytoid DC (pDC) counts (80% vs. 61%; p = 0.006) at post treatment time points. mDC2 phenotypes were more prevalent, compared with baseline, following combination treatment (0.16% vs. 0.05%; p = 0.002). Oral intake of AHCC® and BB536 may modulate T regulatory and DC phenotypes to favour anti-inflammatory responses following antibiotic usage.

Citation

Chowdhury, A. H., Cámara, M., Verma, C., Eremin, O., Kulkarni, A. D., & Lobo, D. N. (2019). Modulation of T regulatory and dendritic cell phenotypes following ingestion of Bifidobacterium longum, active hexose correlated compound (AHCC®) and azithromycin in healthy individuals. Nutrients, 11(10), Article 2470. https://doi.org/10.3390/nu11102470

• Journal Article Type: Article
• Acceptance Date: Oct 9, 2019
• Online Publication Date: Oct 15, 2019
• Publication Date: Oct 15, 2019
• Deposit Date: Oct 16, 2019
• Publicly Available Date: Oct 16, 2019
• Journal: Nutrients
• Electronic ISSN: 2072-6643
• Publisher: MDPI
• Peer Reviewed: Peer Reviewed
• Volume: 11
• Issue: 10
• Article Number: 2470
• DOI: https://doi.org/10.3390/nu11102470
• Keywords: probiotics; prebiotics; synbiotics; antibiotics; dendritic cells; immunity; Bifidobacterium
• Public URL: https://nottingham-repository.worktribe.com/output/2847681
• Publisher URL: https://www.mdpi.com/2072-6643/11/10/2470
• Contract Date: Oct 16, 2019