Analysis of endothelial progenitor cell subtypes as clinical biomarkers for elderly patients with ischaemic stroke

Kadir, Rais Reskiawan A.; Rakkar, Kamini; Othman, Othman A.; Sprigg, Nikola; Bath, Philip M.; Bayraktutan, Ulvi

Abstract

Endothelial progenitor cells (EPCs), expressing markers for stemness (CD34), immaturity (CD133) and endothelial maturity (KDR), may determine the extent of post-stroke vascular repair. Given the prevalence of stroke in elderly, this study explored whether variations in plasmatic availability of certain EPC subtypes could predict the severity and outcome of disease in older patients. Blood samples were collected from eighty-one consented patients (≥ 65years) at admission and days 7, 30 and 90 post-stroke. EPCs were counted with flow cytometry. Stroke severity and outcome were assessed using the National Institutes of Health Stroke Scale, Barthel Index and modified Rankin Scale. The levels of key elements known to affect EPC characteristics were measured by ELISA. Diminished total antioxidant capacity and CD34 + KDR + and CD133 + KDR + counts in early phases of stroke were associated with disease severity and worse functional outcome at day 90 post-stroke. Baseline levels of angiogenic agent PDGF-BB, but not VEGF, positively correlated with CD34 + KDR + numbers at day 90. Baseline LDL-cholesterol levels were inversely correlated with CD34 + KDR+, CD133 + KDR + and CD34 + CD133 + KDR + numbers at day 90. Close correlation between baseline CD34 + KDR + and CD133 + KDR + counts and the outcome of stroke proposes these particular EPC subtypes as potential prognostic markers for ischaemic stroke.

Citation

Kadir, R. R. A., Rakkar, K., Othman, O. A., Sprigg, N., Bath, P. M., & Bayraktutan, U. (2023). Analysis of endothelial progenitor cell subtypes as clinical biomarkers for elderly patients with ischaemic stroke. Scientific Reports, 13(1), Article 21843. https://doi.org/10.1038/s41598-023-48907-7

• Journal Article Type: Article
• Acceptance Date: Dec 1, 2023
• Online Publication Date: Dec 9, 2023
• Publication Date: Dec 9, 2023
• Deposit Date: Feb 18, 2024
• Publicly Available Date: Feb 19, 2024
• Journal: Scientific Reports
• Electronic ISSN: 2045-2322
• Publisher: Nature Publishing Group
• Peer Reviewed: Peer Reviewed
• Volume: 13
• Issue: 1
• Article Number: 21843
• DOI: https://doi.org/10.1038/s41598-023-48907-7
• Keywords: Multidisciplinary
• Public URL: https://nottingham-repository.worktribe.com/output/28416573
• Publisher URL: https://www.nature.com/articles/s41598-023-48907-7
• Additional Information: Received: 18 August 2023; Accepted: 1 December 2023; First Online: 9 December 2023; : The authors declare no competing interests.