Ratiometric detection of enzyme turnover and flavin reduction using rare-earth upconverting phosphors

Harvey, Peter; Oakland, Chlo�; Driscoll, Max D.; Hay, Sam; Natrajan, Louise S.

Abstract

Gd4O2S:Yb:Tm rare-earth upconversion phosphors have been utilised to monitor the redox behaviour of flavin mononucleotide and report on the turnover of a flavo-protein, (pentaerythritol tetranitrate reductase). The presence of two bands separated by over 300 nm in the UCP emission spectra allows ratiometric signalling of these processes with high sensitivity.

Citation

Harvey, P., Oakland, C., Driscoll, M. D., Hay, S., & Natrajan, L. S. (2014). Ratiometric detection of enzyme turnover and flavin reduction using rare-earth upconverting phosphors. Dalton Transactions, 43(14), 5265-5268. https://doi.org/10.1039/c4dt00356j

• Journal Article Type: Article
• Acceptance Date: Feb 7, 2014
• Online Publication Date: Feb 7, 2014
• Publication Date: Feb 7, 2014
• Deposit Date: Oct 23, 2019
• Publicly Available Date: Nov 1, 2019
• Journal: Dalton Trans.
• Print ISSN: 1477-9226
• Electronic ISSN: 1477-9234
• Publisher: Royal Society of Chemistry
• Peer Reviewed: Peer Reviewed
• Volume: 43
• Issue: 14
• Pages: 5265-5268
• DOI: https://doi.org/10.1039/c4dt00356j
• Public URL: https://nottingham-repository.worktribe.com/output/2829462
• Publisher URL: https://pubs.rsc.org/en/content/articlelanding/2014/DT/C4DT00356J#!divAbstract
• Additional Information: : This document is CrossCheck deposited; : Supplementary Information; : The Royal Society of Chemistry has an exclusive publication licence for this journal; OPEN ACCESS: The accepted version of this article will be made freely available after a 12 month embargo period; : Received 3 February 2014; Accepted 7 February 2014; Accepted Manuscript published 7 February 2014; Advance Article published 17 February 2014; Version of Record published 11 March 2014
• Contract Date: Oct 23, 2019