Skip to main content

Research Repository

Advanced Search

De novo transcriptome assembly of hyperaccumulating Noccaea praecox for gene discovery

Bočaj, Valentina; Pongrac, Paula; Fischer, Sina; Likar, Matevž

De novo transcriptome assembly of hyperaccumulating Noccaea praecox for gene discovery Thumbnail


Authors

Valentina Bočaj

Paula Pongrac

Matevž Likar



Abstract

Hyperaccumulators are a group of plant species that accumulate high concentrations of one or more metal(loid)s in their above-ground tissues without showing any signs of toxicity. Several hyperaccumulating species belong to the Brassicaceae family, among them the Cd and Zn hyperaccumulator Noccaea praecox. In this paper, we present de novo transcriptome assembled from two naturally occurring N. praecox populations growing in (i) metal-enriched soil and (ii) soil non-contaminated with metals (control site). Total RNA was extracted from the leaves of both populations. We obtained 801,935,101 reads, which were successfully assembled and annotated. The resulting assembly contains 135,323 transcripts, with 103,396 transcripts (76.4%) annotated with at least one function and encoding 53,142 putative proteins. Due to its close relationship with the hyperaccumulating model species N. cearulescens, it will be possible to derive protein functions from sequence comparisons with this species. Comparisons will highlight common and differing pathways of metal acquisition, storage, and detoxification which will allow us to expand our knowledge of these processes.

Citation

Bočaj, V., Pongrac, P., Fischer, S., & Likar, M. (2023). De novo transcriptome assembly of hyperaccumulating Noccaea praecox for gene discovery. Scientific Data, 10, Article 856. https://doi.org/10.1038/s41597-023-02776-x

Journal Article Type Article
Acceptance Date Nov 23, 2023
Online Publication Date Dec 1, 2023
Publication Date Dec 1, 2023
Deposit Date Feb 9, 2024
Publicly Available Date Feb 12, 2024
Journal Scientific Data
Electronic ISSN 2052-4463
Publisher Nature Publishing Group
Peer Reviewed Peer Reviewed
Volume 10
Article Number 856
DOI https://doi.org/10.1038/s41597-023-02776-x
Public URL https://nottingham-repository.worktribe.com/output/28133291
Publisher URL https://www.nature.com/articles/s41597-023-02776-x

Files





You might also like



Downloadable Citations