Interspecies control of development during mammalian gastrulation

Simpson, Luke; Alberio, Ramiro

Abstract

Gastrulation represents a pivotal phase of development and aberrations during this period can have major consequences, from minor anatomical deviations to severe congenital defects. Animal models are used to study gastrulation, however, there is considerable morphological and molecular diversity of gastrula across mammalian species. Here, we provide an overview of the latest research on interspecies developmental control across mammals. This includes single-cell atlases of several mammalian gastrula which have enabled comparisons of the temporal and molecular dynamics of differentiation. These studies highlight conserved cell differentiation regulators and both absolute and relative differences in differentiation dynamics between species. Recent advances in in vitro culture techniques have facilitated the derivation, maintenance and differentiation of cell lines from a range of species and the creation of multi-species models of gastrulation. Gastruloids are three-dimensional aggregates capable of self-organising and recapitulating aspects of gastrulation. Such models enable species comparisons outside the confines of the embryo. We highlight recent in vitro evidence that differentiation processes such as somitogenesis and neuronal maturation scale with known in vivo differences in developmental tempo across species. This scaling is likely due to intrinsic differences in cell biochemistry. We also highlight several studies which provide examples of cell differentiation dynamics being influenced by extrinsic factors, including culture conditions, chimeric co-culture, and xenotransplantation. These collective studies underscore the complexity of gastrulation across species, highlighting the necessity of additional datasets and studies to decipher the intricate balance between intrinsic cellular programs and extrinsic signals in shaping embryogenesis.

Citation

Simpson, L., & Alberio, R. (2023). Interspecies control of development during mammalian gastrulation. Emerging Topics in Life Sciences, 7(4), 397-408. https://doi.org/10.1042/etls20230083

• Journal Article Type: Review
• Acceptance Date: Oct 23, 2023
• Online Publication Date: Nov 7, 2023
• Publication Date: 2023-12
• Deposit Date: Jan 25, 2024
• Journal: Emerging Topics in Life Sciences
• Print ISSN: 2397-8554
• Electronic ISSN: 2397-8562
• Publisher: Portland Press
• Peer Reviewed: Peer Reviewed
• Volume: 7
• Issue: 4
• Pages: 397-408
• DOI: https://doi.org/10.1042/etls20230083
• Keywords: General Agricultural and Biological Sciences, General Biochemistry, Genetics and Molecular Biology
• Public URL: https://nottingham-repository.worktribe.com/output/27583263
• Publisher URL: https://portlandpress.com/emergtoplifesci/article/7/4/397/233727/Interspecies-control-of-development-during