A simple method for testing and controlling inhibition in soil and sediment samples for qPCR

Bott, Tom; Shaw, George; Gregory, Simon

Abstract

The presence of polymerase-chain-reaction (PCR) inhibitors in many environmental samples can make reliable and repeatable quantitative-polymerase-chain-reaction (qPCR) analysis difficult without sample dilution. To estimate an optimal sample dilution for qPCR and reduce effects of inhibition, a simple test based on multiple dilution series of samples is presented that avoids the use of internal controls and standards reducing complexity and cost.

Citation

Bott, T., Shaw, G., & Gregory, S. (2023). A simple method for testing and controlling inhibition in soil and sediment samples for qPCR. Journal of Microbiological Methods, 212, Article 106795. https://doi.org/10.1016/j.mimet.2023.106795

• Journal Article Type: Article
• Acceptance Date: Aug 2, 2023
• Online Publication Date: Aug 3, 2023
• Publication Date: 2023-09
• Deposit Date: Nov 2, 2023
• Publicly Available Date: Nov 2, 2023
• Journal: Journal of Microbiological Methods
• Print ISSN: 0167-7012
• Electronic ISSN: 1872-8359
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 212
• Article Number: 106795
• DOI: https://doi.org/10.1016/j.mimet.2023.106795
• Keywords: Method optimisation, Inhibition, Quantitative-PCR (qPCR)
• Public URL: https://nottingham-repository.worktribe.com/output/24569801
• Publisher URL: https://www.sciencedirect.com/science/article/pii/S016770122300129X?via%3Dihub