Role of Self‐Assembly Conditions and Amphiphilic Balance on Nanoparticle Formation of PEG‐PDLLA Copolymers in Aqueous Environments

Phan, Hien; Minut, Robert I.; McCrorie, Phoebe; Vasey, Catherine; Larder, Ryan R.; Krumins, Eduards; Marlow, Maria; Rahman, Ruman; Alexander, Cameron; Taresco, Vincenzo; Pearce, Amanda K.

Abstract

The production of well-defined and reproducible poly-meric nanoparticles (NPs), in terms of size and stability in biological environments, is undoubtedly a fundamental challenge in the formulation of novel and more effective nanomedicines. The adoption of PEGylated lactide (LA) block copolymers as biodegradable and biocompatible nanocarriers at different clinical stages has rendered these materials an attractive polymeric platform to be exploited and their formulation is further understood. In the present work, we synthesized a library of linear polyethyl-ene glycol-poly(D,L-lactide) block copolymers with different lengths of LA (15, 25, 50, and 100 LA units) via simple and metal-free ring-opening polymerization, in order to alter the amphi-philic balance of the different macromolecules. The produced polymers were formulated into NPs while varying a series of key parameters in the solvent displacement process, including solvent:nonsolvent ratios and the nature of the two media, and the effect on size and stability was assessed. In addition, stability to protein-NPs interaction and aggregation was studied, highlighting the different NP final properties according to the nature of the amphiphilic balance and nanoformulation conditions. Therefore, we have illustrated a systematic and methodo-logical process to optimize a series of NPs parameters balancing particle size, size distribution, surface charge, and stability to guide future works in the nanoformulation field.

Citation

Phan, H., Minut, R. I., McCrorie, P., Vasey, C., Larder, R. R., Krumins, E., Marlow, M., Rahman, R., Alexander, C., Taresco, V., & Pearce, A. K. (2019). Role of Self‐Assembly Conditions and Amphiphilic Balance on Nanoparticle Formation of PEG‐PDLLA Copolymers in Aqueous Environments. Journal of Polymer Science Part A: Polymer Chemistry, 57(17), 1801-1810. https://doi.org/10.1002/pola.29451

• Journal Article Type: Article
• Acceptance Date: Jul 19, 2019
• Online Publication Date: Aug 2, 2019
• Publication Date: Sep 1, 2019
• Deposit Date: Aug 2, 2019
• Publicly Available Date: Aug 5, 2019
• Journal: Journal of Polymer Science Part A: Polymer Chemistry
• Print ISSN: 0887-624X
• Electronic ISSN: 1099-0518
• Publisher: Wiley
• Peer Reviewed: Peer Reviewed
• Volume: 57
• Issue: 17
• Pages: 1801-1810
• DOI: https://doi.org/10.1002/pola.29451
• Keywords: Organic chemistry; Materials chemistry; Polymers and plastics
• Public URL: https://nottingham-repository.worktribe.com/output/2381392
• Publisher URL: https://onlinelibrary.wiley.com/doi/full/10.1002/pola.29451
• Contract Date: Aug 2, 2019