PUCHI regulates very long chain fatty acid biosynthesis during lateral root and callus formation

Trinh, Duy-Chi; Lavenus, Julien; Goh, Tatsuaki; Boutté, Yohann; Drogue, Quentin; Vaissayre, Virginie; Tellier, Frédérique; Lucas, Mikaël; Voß, Ute; Gantet, Pascal; Faure, Jean-Denis; Dussert, Stéphane; Fukaki, Hidehiro; Bennett, Malcolm J.; Laplaze, Laurent; Guyomarc’h, Soazig

Abstract

© 2019 National Academy of Sciences. All rights reserved. Lateral root organogenesis plays an essential role in elaborating plant root system architecture. In Arabidopsis, the AP2 family transcription factor PUCHI controls cell proliferation in lateral root primordia. To identify potential targets of PUCHI, we analyzed a time course transcriptomic dataset of lateral root formation. We report that multiple genes coding for very long chain fatty acid (VLCFA) biosynthesis enzymes are induced during lateral root development in a PUCHI-dependent manner. Significantly, several mutants perturbed in VLCFA biosynthesis show similar lateral root developmental defects as puchi-1. Moreover, puchi-1 roots display the same disorganized callus formation phenotype as VLCFA biosynthesis-deficient mutants when grown on auxin-rich callus-inducing medium. Lipidomic profiling of puchi-1 roots revealed reduced VLCFA content compared with WT. We conclude that PUCHI-regulated VLCFA biosynthesis is part of a pathway controlling cell proliferation during lateral root and callus formation.

Citation

Trinh, D.-C., Lavenus, J., Goh, T., Boutté, Y., Drogue, Q., Vaissayre, V., Tellier, F., Lucas, M., Voß, U., Gantet, P., Faure, J.-D., Dussert, S., Fukaki, H., Bennett, M. J., Laplaze, L., & Guyomarc’h, S. (2019). PUCHI regulates very long chain fatty acid biosynthesis during lateral root and callus formation. Proceedings of the National Academy of Sciences, 116(28), 14325-14330. https://doi.org/10.1073/pnas.1906300116

• Journal Article Type: Article
• Acceptance Date: Jun 4, 2019
• Online Publication Date: Jun 24, 2019
• Publication Date: Jun 24, 2019
• Deposit Date: Jun 28, 2019
• Publicly Available Date: Jul 1, 2019
• Journal: Proceedings of the National Academy of Sciences
• Print ISSN: 0027-8424
• Electronic ISSN: 1091-6490
• Publisher: National Academy of Sciences
• Peer Reviewed: Peer Reviewed
• Volume: 116
• Issue: 28
• Pages: 14325-14330
• DOI: https://doi.org/10.1073/pnas.1906300116
• Keywords: Multidisciplinary
• Public URL: https://nottingham-repository.worktribe.com/output/2230912
• Publisher URL: https://www.pnas.org/content/early/2019/06/20/1906300116
• Contract Date: Jun 28, 2019