Impact of transcutaneous neuromuscular electrical stimulation or resistance exercise on skeletal muscle mRNA expression in COPD

Latimer, Lorna; Constantin, Despina; Greening, Neil; Calvert, Lori; Menon, Manoj; Greenhaff, Paul

Abstract

Background: Voluntary resistance exercise (RE) training increases muscle mass and strength in patients with chronic obstructive pulmonary disease (COPD). Nonvolitional transcutaneous neuromuscular electrical stimulation (NMES) may be an alternative strategy for reducing ambulatory muscle weakness in patients unable to perform RE training, but little comparative data are available. This study, therefore, investigated changes in muscle mRNA abundance of a number of gene targets in response to a single bout of NMES compared with RE.
Methods: Twenty-six patients with stable COPD (15 male; FEV1, 43±18% predicted; age, 64±8 years; fat free mass index, 16.6±1.8 kg/m2,) undertook 30 minutes of quadriceps NMES (50 Hz, current at the limit of tolerance) or 5×30 maximal voluntary isokinetic knee extensions. Vastus lateralis muscle biopsies were obtained at rest immediately before and 24 hours after intervention. Expression of 384 targeted mRNA transcripts was assessed by real time TaqMan PCR. Significant change in expression from baseline was determined using the ΔΔCT method with a false discovery rate (FDR) of

Citation

Latimer, L., Constantin, D., Greening, N., Calvert, L., Menon, M., & Greenhaff, P. (2019). Impact of transcutaneous neuromuscular electrical stimulation or resistance exercise on skeletal muscle mRNA expression in COPD. International Journal of Chronic Obstructive Pulmonary Disease, 14, 1355-1364. https://doi.org/10.2147/COPD.S189896

• Journal Article Type: Article
• Acceptance Date: Mar 4, 2019
• Online Publication Date: Jun 28, 2019
• Publication Date: Jun 28, 2019
• Deposit Date: Apr 30, 2019
• Publicly Available Date: Jun 28, 2019
• Journal: International Journal of Chronic Obstructive Pulmonary Disease
• Electronic ISSN: 1178-2005
• Publisher: Dove Press
• Peer Reviewed: Peer Reviewed
• Volume: 14
• Pages: 1355-1364
• DOI: https://doi.org/10.2147/COPD.S189896
• Keywords: COPD; NMES; skeletal muscle; gene expression; resistance exercise
• Public URL: https://nottingham-repository.worktribe.com/output/1888995
• Publisher URL: https://www.dovepress.com/impact-of-transcutaneous-neuromuscular-electrical-stimulation-or-resis-peer-reviewed-article-COPD
• Contract Date: Apr 30, 2019