Richard Harrison
Magnetically Assisted Control of Stem Cells Applied in 2D, 3D and In Situ Models of Cell Migration
Harrison, Richard; Luckett, Jeni; Marsh, Sarah; Lugo Leija, Hilda Anaid; Salih, Shelanah; Alkharji, Reem; Sottile, Virginie
Authors
Dr JENI LUCKETT JENI.LUCKETT@NOTTINGHAM.AC.UK
SENIOR RESEARCH FELLOW
Sarah Marsh
Hilda Anaid Lugo Leija
Shelanah Salih
Reem Alkharji
Virginie Sottile
Abstract
The success of cell therapy approaches is greatly dependent on the ability to precisely deliver and monitor transplanted stem cell grafts at treated sites. Iron oxide particles, traditionally used in vivo for magnetic resonance imaging (MRI), have been shown to also represent a safe and efficient in vitro labelling agent for mesenchymal stem cells (MSCs). Here, stem cells were labelled with magnetic particles, and their resulting response to magnetic forces was studied using 2D and 3D models. Labelled cells exhibited magnetic responsiveness, which promoted localised retention and patterned cell seeding when exposed to magnet arrangements in vitro. Directed migration was observed in 2D culture when adherent cells were exposed to a magnetic field, and also when cells were seeded into a 3D gel. Finally, a model of cell injection into the rodent leg was used to test the enhanced localised retention of labelled stem cells when applying magnetic forces, using whole body imaging to confirm the potential use of magnetic particles in strategies seeking to better control cell distribution for in vivo cell delivery.
Citation
Harrison, R., Luckett, J., Marsh, S., Lugo Leija, H. A., Salih, S., Alkharji, R., & Sottile, V. (2019). Magnetically Assisted Control of Stem Cells Applied in 2D, 3D and In Situ Models of Cell Migration. Molecules, 24(8), Article 1563. https://doi.org/10.3390/molecules24081563
Journal Article Type | Article |
---|---|
Acceptance Date | Apr 18, 2019 |
Online Publication Date | Apr 19, 2019 |
Publication Date | Apr 2, 2019 |
Deposit Date | Apr 25, 2019 |
Publicly Available Date | Apr 25, 2019 |
Journal | Molecules |
Electronic ISSN | 1420-3049 |
Publisher | MDPI |
Peer Reviewed | Peer Reviewed |
Volume | 24 |
Issue | 8 |
Article Number | 1563 |
DOI | https://doi.org/10.3390/molecules24081563 |
Keywords | Organic Chemistry |
Public URL | https://nottingham-repository.worktribe.com/output/1841113 |
Publisher URL | https://www.mdpi.com/1420-3049/24/8/1563 |
Contract Date | Apr 25, 2019 |
Files
molecules-24-01563
(8 Mb)
PDF
Publisher Licence URL
https://creativecommons.org/licenses/by/4.0/
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