Genome-scale model of C. autoethanogenum reveals optimal bioprocess conditions for high-value chemical production from carbon monoxide

Norman, Rupert O.J.; Millat, Thomas; Schatschneider, Sarah; Henstra, Anne M.; Breitkopf, Ronja; Pander, Bart; Annan, Florence J.; Piatek, Pawel; Hartman, Hassan B.; Poolman, Mark G.; Fell, David A.; Winzer, Klaus; Minton, Nigel P.; Hodgman, Charlie

Abstract

Clostridium autoethanogenum is an industrial microbe used for the commercial-scale production of ethanol from carbon monoxide. While significant progress has been made in the attempted diversification of this bioprocess, further improvements are desirable, particularly in the formation of the high-value platform chemicals, such as 2,3-butanediol. A new, experimentally parameterised genome scale model of C. autoethanogenum predicts dramatically increased 2,3-butanediol production under non-carbon-limited conditions when thermodynamic constraints on hydrogen production are considered.

Citation

Norman, R. O., Millat, T., Schatschneider, S., Henstra, A. M., Breitkopf, R., Pander, B., Annan, F. J., Piatek, P., Hartman, H. B., Poolman, M. G., Fell, D. A., Winzer, K., Minton, N. P., & Hodgman, C. (2019). Genome-scale model of C. autoethanogenum reveals optimal bioprocess conditions for high-value chemical production from carbon monoxide. Engineering Biology, 3(2), 32-40. https://doi.org/10.1049/enb.2018.5003

• Journal Article Type: Article
• Acceptance Date: Mar 20, 2019
• Online Publication Date: Apr 17, 2019
• Publication Date: Jun 30, 2019
• Deposit Date: Apr 11, 2019
• Publicly Available Date: Jun 24, 2019
• Journal: Engineering Biology
• Electronic ISSN: 2398-6182
• Publisher: Institution of Engineering and Technology (IET)
• Peer Reviewed: Peer Reviewed
• Volume: 3
• Issue: 2
• Pages: 32-40
• DOI: https://doi.org/10.1049/enb.2018.5003
• Public URL: https://nottingham-repository.worktribe.com/output/1778211
• Publisher URL: https://digital-library.theiet.org/content/journals/10.1049/enb.2018.5003
• Contract Date: Jun 24, 2019