An in-vivo pilot study into the effects of FDG-mNP in cancer in mice

Aras, Omer; Pearce, Gillian; Watkins, Adam J.; Nurili, Fuad; Medine, Emin Ilker; Guldu, Ozge Kozgus; Tekin, Volkan; Wong, Julian; Ma, Xianghong; Ting, Richard; Unak, Perihan; Akin, Oguz

Abstract

PURPOSE:
Previously, fluorodeoxy glucose conjugated magnetite nanoparticles (FDG-mNPs) injected into cancer cells in conjunction with the application of magnetic hyperthermia have shown promise in new FDG-mNPs applications. The aim of this study was to determine potential toxic or unwanted effects involving both tumour cells and normal tissue in other organs when FDG-mNPs are administered intravenously or intratumourally in mice.

MATERIALS AND METHODS:
FDG-mNPs were synthesized. A group of six prostate-tumour bearing mice were injected with 23.42 mg/ml FDG-mNPs (intravenous injection, n = 3; intratumoural injection into the prostate tumour, n = 3). Mice were euthanized and histological sampling of tissue was conducted for the prostate tumour, as well as for lungs, lymph nodes, liver, kidneys, spleen, and brain, at 1 hour (n = 2) and 7 days (n = 4) post-injection. A second group of two normal (non-cancerous) mice received the same injection intravenously into the tail vein and were euthanised at 3 and 6 months post-injection, respectively, to investigate if FDG-mNPs remained in organs at those time points.

RESULTS:
In prostate-tumour bearing mice, FDG-mNPs concentrated in the prostate tumour, while relatively small amounts were found in the organs of other tissues, particularly the spleen and the liver; FDG-mNP concentrations decreased over time in all tissues. In normal mice, no detrimental effects were found in either mouse at 3 or 6 months.

CONCLUSION:
Intravenous or intratumoural FDG-mNPs can be safely administered for effective cancer cell destruction. Further research on the clinical utility of FDG-mNPs will be conducted by applying hyperthermia in conjunction with FDG-mNPs in mice.

Citation

Aras, O., Pearce, G., Watkins, A. J., Nurili, F., Medine, E. I., Guldu, O. K., Tekin, V., Wong, J., Ma, X., Ting, R., Unak, P., & Akin, O. (2018). An in-vivo pilot study into the effects of FDG-mNP in cancer in mice. PLoS ONE, 13(8), Article e0202482. https://doi.org/10.1371/journal.pone.0202482

• Journal Article Type: Article
• Acceptance Date: Aug 10, 2018
• Online Publication Date: Aug 20, 2018
• Publication Date: Aug 21, 2018
• Deposit Date: Aug 21, 2018
• Publicly Available Date: Aug 21, 2018
• Journal: PLOS ONE
• Publisher: Public Library of Science
• Peer Reviewed: Peer Reviewed
• Volume: 13
• Issue: 8
• Article Number: e0202482
• DOI: https://doi.org/10.1371/journal.pone.0202482
• Keywords: General Biochemistry, Genetics and Molecular Biology; General Agricultural and Biological Sciences; General Medicine
• Public URL: https://nottingham-repository.worktribe.com/output/1040923
• Publisher URL: http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0202482
• Contract Date: Aug 21, 2018