Outputs (5)

Low-temperature vacuum evaporation as a novel dehydration process for the long-term preservation of transplantable human corneal tissue (2025)
Journal Article
McIntosh, O. D., Britchford, E. R., Beeken, L. J., Hopkinson, A., & Sidney, L. E. (2025). Low-temperature vacuum evaporation as a novel dehydration process for the long-term preservation of transplantable human corneal tissue. Cell and Tissue Banking, 26(1), Article 7. https://doi.org/10.1007/s10561-024-10155-y

Globally there is a shortage of available donor corneas with only 1 cornea available for every 70 needed. A large limitation to corneal transplant surgery is access to quality donor tissue due to inadequate eye donation services and infrastructure in... Read More about Low-temperature vacuum evaporation as a novel dehydration process for the long-term preservation of transplantable human corneal tissue.

Comparative cytotoxicity of Acanthamoeba castellanii-derived conditioned medium on human corneal epithelial and stromal cells (2024)
Journal Article
Alhazmi, A., Sidney, L. E., Hopkinson, A., & Elsheikha, H. M. (2024). Comparative cytotoxicity of Acanthamoeba castellanii-derived conditioned medium on human corneal epithelial and stromal cells. Acta Tropica, 257, Article 107288. https://doi.org/10.1016/j.actatropica.2024.107288

Soluble factors in the secretome of Acanthamoeba castellanii play crucial roles in the pathogenesis of Acanthamoeba keratitis (AK). Investigating the pathological effects of A. castellanii-derived conditioned medium (ACCM) on ocular cells can provide... Read More about Comparative cytotoxicity of Acanthamoeba castellanii-derived conditioned medium on human corneal epithelial and stromal cells.

Increased Anti-Inflammatory Therapeutic Potential and Progenitor Marker Expression of Corneal Mesenchymal Stem Cells Cultured in an Optimized Propagation Medium (2024)
Journal Article
Hopkinson, A., Notara, M., Cursiefen, C., & Sidney, L. E. (2024). Increased Anti-Inflammatory Therapeutic Potential and Progenitor Marker Expression of Corneal Mesenchymal Stem Cells Cultured in an Optimized Propagation Medium. Cell Transplantation, 33, 1-17. https://doi.org/10.1177/09636897241241992

There is a huge unmet need for new treatment modalities for ocular surface inflammatory disorders (OSIDs) such as dry eye disease and meibomian gland dysfunction. Mesenchymal stem cell therapies may hold the answer due to their potent immunomodulator... Read More about Increased Anti-Inflammatory Therapeutic Potential and Progenitor Marker Expression of Corneal Mesenchymal Stem Cells Cultured in an Optimized Propagation Medium.

Endothelial cell loss following tissue harvesting by pneumodissection for endothelial keratoplasty: an ex vivo study. (2015)
Journal Article
SL, A., A, G., LE, S., MS, E., A, A., & HS, D. (2015). Endothelial cell loss following tissue harvesting by pneumodissection for endothelial keratoplasty: an ex vivo study. British Journal of Ophthalmology, 99(5), 710-713. https://doi.org/10.1136/bjophthalmol-2014-306560

Aims
To study ex vivo the difference in endothelial cell density (ECD) in tissue harvested by pneumodissection for pre-Descemet's endothelial keratoplasty (PDEK) and Descemet's membrane endothelial keratoplasty (DMEK).

Methods
Tissue for PDEK... Read More about Endothelial cell loss following tissue harvesting by pneumodissection for endothelial keratoplasty: an ex vivo study..

Keeping an Eye on Decellularized Corneas: A Review of Methods, Characterization and Applications (2013)
Journal Article
Wilson, S., Sidney, L., Dunphy, S., Rose, J., & Hopkinson, A. (2013). Keeping an Eye on Decellularized Corneas: A Review of Methods, Characterization and Applications. Journal of Functional Biomaterials, 4(3), 114-161. https://doi.org/10.3390/jfb4030114

The worldwide limited availability of suitable corneal donor tissue has led to the development of alternatives, including keratoprostheses (Kpros) and tissue engineered (TE) constructs. Despite advances in bioscaffold design, there is yet to be a cor... Read More about Keeping an Eye on Decellularized Corneas: A Review of Methods, Characterization and Applications.