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Outputs (7)

Computer Vision for Substrate Detection in High-Throughput Biomaterial Screens Using Bright-Field Microscopy (2024)
Journal Article
Owen, R., Nasir, A., H. Amer, M., Nie, C., Xue, X., Burroughs, L., Denning, C., D. Wildman, R., A. Khan, F., R. Alexander, M., & R. A. J. Rose, F. (2024). Computer Vision for Substrate Detection in High-Throughput Biomaterial Screens Using Bright-Field Microscopy. Advanced Intelligent Systems, Article 2400573. https://doi.org/10.1002/aisy.202400573

High-throughput screening (HTS) can be used when ab initio information is unavailable for rational design of new materials, generating data on properties such as chemistry and topography that control cell behavior. Biomaterial screens are typically f... Read More about Computer Vision for Substrate Detection in High-Throughput Biomaterial Screens Using Bright-Field Microscopy.

SnoRNAs in cardiovascular development, function, and disease (2024)
Journal Article
Chabronova, A., Holmes, T. L., Hoang, D. M., Denning, C., James, V., Smith, J. G. W., & Peffers, M. J. (2024). SnoRNAs in cardiovascular development, function, and disease. Trends in Molecular Medicine, 30(6), 562-578. https://doi.org/10.1016/j.molmed.2024.03.004

Small nucleolar RNAs (snoRNAs) are emerging as important regulators of cardiovascular (patho)biology. Several roles of snoRNAs have recently been identified in heart development and congenital heart diseases, as well as their dynamic regulation in hy... Read More about SnoRNAs in cardiovascular development, function, and disease.

Strengthening cardiac therapy pipelines using human pluripotent stem cell-derived cardiomyocytes (2024)
Journal Article
Raniga, K., Nasir, A., Vo, N. T., Vaidyanathan, R., Dickerson, S., Hilcove, S., Mosqueira, D., Mirams, G. R., Clements, P., Hicks, R., Pointon, A., Stebbeds, W., Francis, J., & Denning, C. (2024). Strengthening cardiac therapy pipelines using human pluripotent stem cell-derived cardiomyocytes. Cell Stem Cell, 31(3), 292-311. https://doi.org/10.1016/j.stem.2024.01.007

Advances in hiPSC isolation and reprogramming and hPSC-CM differentiation have prompted their therapeutic application and utilization for evaluating potential cardiovascular safety liabilities. In this perspective, we showcase key efforts toward the... Read More about Strengthening cardiac therapy pipelines using human pluripotent stem cell-derived cardiomyocytes.

Perlecan (HSPG2) promotes structural, contractile, and metabolic development of human cardiomyocytes (2024)
Journal Article
Johnson, B. B., Cosson, M.-V., Tsansizi, L. I., Holmes, T. L., Gilmore, T., Hampton, K., Song, O.-R., Vo, N. T. N., Nasir, A., Chabronova, A., Denning, C., Peffers, M. J., Merry, C. L. R., Whitelock, J., Troeberg, L., Rushworth, S. A., Bernardo, A. S., & Smith, J. G. W. (2024). Perlecan (HSPG2) promotes structural, contractile, and metabolic development of human cardiomyocytes. Cell Reports, 43(1), Article 113668. https://doi.org/10.1016/j.celrep.2023.113668

Perlecan (HSPG2), a heparan sulfate proteoglycan similar to agrin, is key for extracellular matrix (ECM) maturation and stabilization. Although crucial for cardiac development, its role remains elusive. We show that perlecan expression increases as c... Read More about Perlecan (HSPG2) promotes structural, contractile, and metabolic development of human cardiomyocytes.

Human model of primary carnitine deficiency cardiomyopathy reveals ferroptosis as a novel mechanism (2023)
Journal Article
Loos, M., Klampe, B., Schulze, T., Yin, X., Theofilatos, K., Ulmer, B. M., Schulz, C., Behrens, C. S., van Bergen, T. D., Adami, E., Maatz, H., Schweizer, M., Brodesser, S., Skryabin, B. V., Rozhdestvensky, T. S., Bodbin, S., Stathopoulou, K., Christ, T., Denning, C., Hübner, N., …Hansen, A. (2023). Human model of primary carnitine deficiency cardiomyopathy reveals ferroptosis as a novel mechanism. Stem Cell Reports, 18(11), 2123-2137. https://doi.org/10.1016/j.stemcr.2023.09.002

Primary carnitine deficiency (PCD) is an autosomal recessive monogenic disorder caused by mutations in SLC22A5. This gene encodes for OCTN2, which transports the essential metabolite carnitine into the cell. PCD patients suffer from muscular weakness... Read More about Human model of primary carnitine deficiency cardiomyopathy reveals ferroptosis as a novel mechanism.

Surface Plasmon Resonance Imaging of Excitable Cells (2018)
Preprint / Working Paper
Howe, C. L., Webb, K. F., Abayzeed, S. A., Anderson, D. J., Denning, C., & Russell, N. A. Surface Plasmon Resonance Imaging of Excitable Cells

Surface plasmons are highly sensitive to refractive index variations adjacent to the surface. This sensitivity has been exploited successfully for chemical and biological assays. In these systems, a surface plasmon resonance (SPR)-based sensor detect... Read More about Surface Plasmon Resonance Imaging of Excitable Cells.