Outputs (20)

Engineering the Human Fc Region Enables Direct Cell Killing by Cancer Glycan-Targeting Antibodies without the Need for Immune Effector Cells or Complement (2020)
Journal Article
Vankemmelbeke, M., McIntosh, R. S., Chua, J. X., Kirk, T., Daniels, I., Patsalidou, M., Moss, R., Parsons, T., Scott, D., Harris, G., Ramage, J. M., Spendlove, I., & Durrant, L. G. (2020). Engineering the Human Fc Region Enables Direct Cell Killing by Cancer Glycan-Targeting Antibodies without the Need for Immune Effector Cells or Complement. Cancer Research, 80(16), 3399-3412. https://doi.org/10.1158/0008-5472.CAN-19-3599

©2020 American Association for Cancer Research. Murine IgG3 glycan-targeting mAb often induces direct cell killing in the absence of immune effector cells or complement via a proinflammatory mechanism resembling oncotic necrosis. This cancer cell kil... Read More about Engineering the Human Fc Region Enables Direct Cell Killing by Cancer Glycan-Targeting Antibodies without the Need for Immune Effector Cells or Complement.

Structural modeling of an outer membrane electron conduit from a metal-reducing bacterium suggests electron transfer via periplasmic redox partners (2018)
Journal Article
Edwards, M. J., White, G. F., Lockwood, C. W., Lawes, M. C., Martel, A., Harris, G., Scott, D. J., Richardson, D. J., Butt, J. N., & Clarke, T. A. (2018). Structural modeling of an outer membrane electron conduit from a metal-reducing bacterium suggests electron transfer via periplasmic redox partners. Journal of Biological Chemistry, 293(21), 8103-8112. https://doi.org/10.1074/jbc.RA118.001850

Many subsurface microorganisms couple their metabolism to the reduction or oxidation of extracellular substrates. For example, anaerobic mineral-respiring bacteria can use external metal oxides as terminal electron acceptors during respiration. Porin... Read More about Structural modeling of an outer membrane electron conduit from a metal-reducing bacterium suggests electron transfer via periplasmic redox partners.

Reconstructing promoter activity from Lux bioluminescent reporters (2017)
Journal Article
Iqbal, M., Doherty, N., Page, A. M., Qazi, S. N., Ajmera, I., Lund, P. A., Kypraios, T., Scott, D. J., Hill, P. J., & Stekel, D. J. (2017). Reconstructing promoter activity from Lux bioluminescent reporters. PLoS Computational Biology, 13(9), Article e1005731. https://doi.org/10.1371/journal.pcbi.1005731

The bacterial Lux system is used as a gene expression reporter. It is fast, sensitive and non-destructive, enabling high frequency measurements. Originally developed for bacterial cells, it has also been adapted for eukaryotic cells, and can be used... Read More about Reconstructing promoter activity from Lux bioluminescent reporters.

Intrinsic disorder in the partitioning protein KorB persists after co-operative complex formation with operator DNA and KorA (2017)
Journal Article
Hyde, E. I., Callow, P., Rajasekar, K. V., Timmins, P., Patel, T. P., Siligardi, G., Hussain, R., White, S. A., Thomas, C. M., & Scott, D. J. (2017). Intrinsic disorder in the partitioning protein KorB persists after co-operative complex formation with operator DNA and KorA. Biochemical Journal, 474(18), 3121-3135. https://doi.org/10.1042/BCJ20170281

© 2017 The Author(s). The ParB protein, KorB, from the RK2 plasmid is required for DNA partitioning and transcriptional repression. It acts co-operatively with other proteins, including the repressor KorA. Like many multifunctional proteins, KorB con... Read More about Intrinsic disorder in the partitioning protein KorB persists after co-operative complex formation with operator DNA and KorA.

Accounting for thermodynamic non-ideality in the Guinier region of small-angle scattering data of proteins (2016)
Journal Article
Scott, D. J. (2016). Accounting for thermodynamic non-ideality in the Guinier region of small-angle scattering data of proteins. Biophysical Reviews, 8(4), https://doi.org/10.1007/s12551-016-0235-5

Hydrodynamic studies of the solution properties of proteins and other biological macromolecules are often hard to interpret when the sample is present at a reasonably concentrated solution. The reason for this is that solutions exhibit deviations fro... Read More about Accounting for thermodynamic non-ideality in the Guinier region of small-angle scattering data of proteins.

STAT2 is a pervasive cytokine regulator due to its inhibition of STAT1 in multiple signaling pathways (2016)
Journal Article
Ho, J., Pelzel, C., Begitt, A., Mee, M., Elsheikha, H. M., Scott, D. J., & Vinkemeier, U. (2016). STAT2 is a pervasive cytokine regulator due to its inhibition of STAT1 in multiple signaling pathways. PLoS Biology, 14(10), 1-27. https://doi.org/10.1371/journal.pbio.2000117

STAT2 is the quintessential transcription factor for type 1 interferons (IFNs), where it functions as a heterodimer with STAT1. However, the human and murine STAT2-deficient phenotypes suggest important additional and currently unidentified type 1 IF... Read More about STAT2 is a pervasive cytokine regulator due to its inhibition of STAT1 in multiple signaling pathways.

SilE is an intrinsically disordered periplasmic ‘molecular sponge' involved in bacterial silver resistance (2016)
Journal Article
Asiani, K. R., Williams, H. E. L., Bird, L., Jenner, M., Searle, M. S., Hobman, J. L., Scott, D. J., & Soultanas, P. (2016). SilE is an intrinsically disordered periplasmic ‘molecular sponge' involved in bacterial silver resistance. Molecular Microbiology, 101(5), 731-742. https://doi.org/10.1111/mmi.13399

Ag+ resistance was initially found on the Salmonella enetrica serovar Typhimurium multi-resistance plasmid pMG101 from burns patients in 1975. The putative model of Ag+ resistance, encoded by the sil operon from pMG101, involves export of Ag+ via an... Read More about SilE is an intrinsically disordered periplasmic ‘molecular sponge' involved in bacterial silver resistance.

Flexibility of KorA, a plasmid-encoded, global transcription regulator, in the presence and the absence of its operator (2016)
Journal Article
Bingle, L. E., Rajasekar, K. V., Lovering, A. L., Dancea, F., Scott, D. J., Harris, S. A., Bingle, L. . E., Roessle, M., Thomas, C. M., Hyde, E. I., & White, S. A. (2016). Flexibility of KorA, a plasmid-encoded, global transcription regulator, in the presence and the absence of its operator. Nucleic Acids Research, 44(10), 4947-4956. https://doi.org/10.1093/nar/gkw191

The IncP (Incompatibility group P) plasmids are important carriers in the spread of antibiotic resistance across Gram-negative bacteria. Gene expression in the IncP-1 plasmids is stringently controlled by a network of four global repressors, KorA, Ko... Read More about Flexibility of KorA, a plasmid-encoded, global transcription regulator, in the presence and the absence of its operator.

Defining the intrinsically disordered C-terminal domain of SSB reveals DNA-mediated compaction (2015)
Journal Article
Green, M., Hatter, L., Brookes, E., Soultanas, P., & Scott, D. (2016). Defining the intrinsically disordered C-terminal domain of SSB reveals DNA-mediated compaction. Journal of Molecular Biology, 428(2), 357-364. https://doi.org/10.1016/j.jmb.2015.12.007

The bacterial single-stranded DNA (ssDNA) binding protein SSB is a strictly conserved and essential protein involved in diverse functions of DNA metabolism, including replication and repair. SSB comprises a well-characterized tetrameric core of N-ter... Read More about Defining the intrinsically disordered C-terminal domain of SSB reveals DNA-mediated compaction.

Comparison of the Structure and Activity of Glycosylated and Aglycosylated Human Carboxylesterase 1 (2015)
Journal Article
Arena de Souza, V., Scott, D. J., Nettleship, J. E., Rahman, N., Charlton, M. H., Walsh, M. A., & Owens, R. J. (2015). Comparison of the Structure and Activity of Glycosylated and Aglycosylated Human Carboxylesterase 1. PLoS ONE, 10(12), e0143919. https://doi.org/10.1371/journal.pone.0143919

Human Carboxylesterase 1 (hCES1) is the key liver microsomal enzyme responsible for detoxification and metabolism of a variety of clinical drugs. To analyse the role of the single N-linked glycan on the structure and activity of the enzyme, authentic... Read More about Comparison of the Structure and Activity of Glycosylated and Aglycosylated Human Carboxylesterase 1.