Outputs (47)

Spatially-offset Raman spectroscopy for monitoring mineralization of bone tissue engineering scaffolds: feasibility study based on phantom samples (2019)
Journal Article
Dooley, M., Prasopthum, A., Liao, Z., Sinjab, F., Mclaren, J., Rose, F. R. A. J., Yang, J., & Notingher, I. (2019). Spatially-offset Raman spectroscopy for monitoring mineralization of bone tissue engineering scaffolds: feasibility study based on phantom samples. Biomedical Optics Express, 10(4), 1678-1690. https://doi.org/10.1364/BOE.10.001678

Using phantom samples, we investigated the feasibility of spatially-offset Raman spectroscopy (SORS) as a tool for monitoring non-invasively the mineralization of bone tissue engineering scaffold in-vivo. The phantom samples consisted of 3D-printed s... Read More about Spatially-offset Raman spectroscopy for monitoring mineralization of bone tissue engineering scaffolds: feasibility study based on phantom samples.

Fast Raman spectral mapping of highly fluorescing samples by time-gated spectral multiplexed detection (2018)
Journal Article
Corden, C. J., Shipp, D. W., Matousek, P., & Notingher, I. (2018). Fast Raman spectral mapping of highly fluorescing samples by time-gated spectral multiplexed detection. Optics Letters, 43(23), 5733-5736. https://doi.org/10.1364/ol.43.005733

We present a time-gated Raman micro-spectroscopy technique suitable for fast Raman mapping of samples eliciting large laser-induced fluorescence backgrounds. To achieve the required time resolution for effective fluorescence rejection, a picosecond p... Read More about Fast Raman spectral mapping of highly fluorescing samples by time-gated spectral multiplexed detection.

Holographic optical trapping Raman micro-spectroscopy for non-invasive measurement and manipulation of live cells (2018)
Journal Article
Sinjab, F., Awuah, D., Gibson, G., Padgett, M., Ghaemmaghami, A. M., & Notingher, I. (2018). Holographic optical trapping Raman micro-spectroscopy for non-invasive measurement and manipulation of live cells. Optics Express, 26(19), 25211-25225. https://doi.org/10.1364/oe.26.025211

We present a new approach for combining holographic optical tweezers with confocal Raman spectroscopy. Multiple laser foci, generated using a liquid-crystal spatial light modulator, are individually used for both optical trapping and excitation of sp... Read More about Holographic optical trapping Raman micro-spectroscopy for non-invasive measurement and manipulation of live cells.

Optical sectioning in multi-foci Raman hyperspectral imaging (2018)
Journal Article
Liao, Z., Sinjab, F., Elsheikha, H. M., & Notingher, I. (2018). Optical sectioning in multi-foci Raman hyperspectral imaging. Journal of Raman Spectroscopy, 49(10), 1660-1667. https://doi.org/10.1002/jrs.5450

In this study, we compared the depth-discrimination and speed performance of multi-foci Raman hyperspectral imaging with the reference standard of a single laser point confocal Raman mapping. A liquid crystal spatial light modulator (LC-SLM) was empl... Read More about Optical sectioning in multi-foci Raman hyperspectral imaging.

Intra-operative spectroscopic assessment of surgical margins during breast conserving surgery (2018)
Journal Article
Shipp, D. W., Rakha, E. A., Koloydenko, A. A., Macmillan, R. D., Ellis, I. O., & Notingher, I. (2018). Intra-operative spectroscopic assessment of surgical margins during breast conserving surgery. Breast Cancer Research, 20, https://doi.org/10.1186/s13058-018-1002-2

Background: In over 20% of breast conserving operations, postoperative pathological assessment of the excised tissue reveals positive margins, requiring additional surgery. Current techniques for intra-operative assessment of tumor margins are insuff... Read More about Intra-operative spectroscopic assessment of surgical margins during breast conserving surgery.

Holographic optical trapping Raman micro-spectroscopy for interacting live cells (2018)
Preprint / Working Paper
Sinjab, F., Elsheikha, H. M., Awuah, D., Gibson, G., Padgett, M., Ghaemmaghami, A. M., & Notingher, I. Holographic optical trapping Raman micro-spectroscopy for interacting live cells

We present a combined holographic optical tweezers and confocal Raman spectroscopy instrument which allows fast, flexible, and interactive manipulation with molecular measurement of interacting live cell systems. Multiple laser foci created using a s... Read More about Holographic optical trapping Raman micro-spectroscopy for interacting live cells.

Visualizing the interaction of Acanthamoeba castellanii with human retinal epithelial cells by spontaneous Raman and CARS imaging (2017)
Journal Article
Naemat, A., Sinjab, F., McDonald, A., Downes, A., Elfick, A., Elsheikha, H. M., & Notingher, I. (2018). Visualizing the interaction of Acanthamoeba castellanii with human retinal epithelial cells by spontaneous Raman and CARS imaging. Journal of Raman Spectroscopy, 49(3), 412-423. https://doi.org/10.1002/jrs.5296

Improved understanding of the mechanism of nutrient’s uptake can enable targeted manipulation of nutrient sensing pathways in medically important pathogens to a greater degree than is currently possible. In this context, we present the use of spontan... Read More about Visualizing the interaction of Acanthamoeba castellanii with human retinal epithelial cells by spontaneous Raman and CARS imaging.

Automated multimodal spectral histopathology for quantitative diagnosis of residual tumour during basal cell carcinoma surgery (2017)
Journal Article
Boitor, R., Kong, K., Shipp, D., Varma, S., Koloydenko, A., Kusum, K., Elsheikh, S., Bakker Schut, T., Caspers, P., Puppels, G., Wolf, M. V. D., Sokolova, E., Nijsten, T., Salence, B., Williams, H. C., & Ioan, N. (in press). Automated multimodal spectral histopathology for quantitative diagnosis of residual tumour during basal cell carcinoma surgery. Biomedical Optics Express, 8(12), https://doi.org/10.1364/BOE.8.005749

Multimodal spectral histopathology (MSH), an optical technique combining tissue auto-fluorescence (AF) imaging and Raman micro-spectroscopy (RMS), was previously proposed for detection of residual basal cell carcinoma (BCC) at the surface of surgical... Read More about Automated multimodal spectral histopathology for quantitative diagnosis of residual tumour during basal cell carcinoma surgery.

Label-free Raman hyperspectral imaging of single cells cultured on polymer substrates (2017)
Journal Article
Sinjab, F., Sicilia, G., Shipp, D., Marlow, M., & Notingher, I. (2017). Label-free Raman hyperspectral imaging of single cells cultured on polymer substrates. Applied Spectroscopy, 71(12), https://doi.org/10.1177/0003702817715042

While Raman hyper-spectral imaging has been widely used for label-free mapping of biomolecules in cells, these measurements require the cells to be cultured on weakly Raman scattering substrates. However, many applications in biological sciences and... Read More about Label-free Raman hyperspectral imaging of single cells cultured on polymer substrates.

Rapid production of human liver scaffolds for functional tissue engineering by high shear stress oscillation-decellularization (2017)
Journal Article
Mazza, G., Al-Akkad, W., Telese, A., Longato, L., Urbani, L., Robinson, B., Hall, A., Kong, K., Frenguelli, L., Marrone, G., Willacy, O., Shaeri, M., Burns, A., Malago, M., Gilbertson, J., Rendell, N., Moore, K., Hughes, D., Notingher, I., Jell, G., …Pinzani, M. (in press). Rapid production of human liver scaffolds for functional tissue engineering by high shear stress oscillation-decellularization. Scientific Reports, 7, Article 5534. https://doi.org/10.1038/s41598-017-05134-1

The development of human liver scaffolds retaining their 3-dimensional structure and extra-cellular matrix (ECM) composition is essential for the advancement of liver tissue engineering. We report the design and validation of a new methodology for th... Read More about Rapid production of human liver scaffolds for functional tissue engineering by high shear stress oscillation-decellularization.