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Outputs (10)

Direct RT-qPCR Assay for the Detection of SARS-CoV-2 in Saliva Samples (2022)
Journal Article
Tarantini, F. S., Wu, S., Jenkins, H., Lopez, A. T., Tomlin, H., Hyde, R., Lis-Slimak, K., Thompson, J. L., Pijuan-Galitó, S., Scales, D., Kaneko, K., Dey, J., Park, E., Hill, J., Lee, I. N., Doolan, L., Arendt-Tranholm, A., Denning, C., Seedhouse, C., & Benest, A. V. (2022). Direct RT-qPCR Assay for the Detection of SARS-CoV-2 in Saliva Samples. Methods and Protocols, 5(2), Article 25. https://doi.org/10.3390/mps5020025

Since mid-2020 there have been complexities and difficulties in the standardisation and administration of nasopharyngeal swabs. Coupled with the variable and/or poor accuracy of lateral flow devices, this has led to increased societal ‘testing fatigu... Read More about Direct RT-qPCR Assay for the Detection of SARS-CoV-2 in Saliva Samples.

Saliva for COVID-19 testing: Simple but useless or an undervalued resource? (2021)
Journal Article
Pijuan-Galito, S., Tarantini, F. S., Tomlin, H., Jenkins, H., Thompson, J. L., Scales, D., Stroud, A., Tellechea Lopez, A., Hassall, J., McTernan, P., Coultas, A., Arendt-Tranholm, A., Reffin, C., Hill, I., Lee, I.-N., Wu, S., Porte, J., Chappell, J., Lis-Slimak, K., Kaneko, K., …Denning, C. (2021). Saliva for COVID-19 testing: Simple but useless or an undervalued resource?. Frontiers in Virology, 1, Article 778790. https://doi.org/10.3389/fviro.2021.778790

During the COVID-19 pandemic, countries with robust population-based asymptomatic testing were generally successful in controlling virus spread, hence reducing hospitalizations and deaths. This effectiveness inspired widespread asymptomatic surveilla... Read More about Saliva for COVID-19 testing: Simple but useless or an undervalued resource?.

METTL3 Regulates Angiogenesis by Modulating let-7e-5p and miRNA-18a-5p Expression in Endothelial Cells (2021)
Journal Article
Chamorro-Jorganes, A., Sweaad, W. K., Katare, R., Besnier, M., Anwar, M., Beazley-Long, N., Sala-Newby, G., Ruiz-Polo, I., Chandrasekera, D., Ritchie, A. A., Benest, A. V., & Emanueli, C. (2021). METTL3 Regulates Angiogenesis by Modulating let-7e-5p and miRNA-18a-5p Expression in Endothelial Cells. Arteriosclerosis, Thrombosis, and Vascular Biology, 41(6), e325-e337. https://doi.org/10.1161/atvbaha.121.316180

Objective:
Postnatal angiogenesis is critical in vascular homeostasis and repair. m6A RNA methylation is emerging as a new layer for fine-tuning gene expression. Although the contribution of the m6A-catalyzing enzyme, METTL3 (methyltransferase-like... Read More about METTL3 Regulates Angiogenesis by Modulating let-7e-5p and miRNA-18a-5p Expression in Endothelial Cells.

Bioinspired artificial exosomes based on lipid nanoparticles carrying let-7b-5p promote angiogenesis in vitro and in vivo (2021)
Journal Article
Aday, S., Hazan-Halevy, I., Chamorro-Jorganes, A., Anwar, M., Goldsmith, M., Beazley-Long, N., Sahoo, S., Dogra, N., Sweaad, W., Catapano, F., Ozaki-Tan, S., Angelini, G. D., Madeddu, P., Benest, A. V., Peer, D., & Emanueli, C. (2021). Bioinspired artificial exosomes based on lipid nanoparticles carrying let-7b-5p promote angiogenesis in vitro and in vivo. Molecular Therapy, 29(7), 2239-2252. https://doi.org/10.1016/j.ymthe.2021.03.015

MicroRNAs (miRNAs) regulate gene expression by post-transcriptional inhibition of target genes. Proangiogenic small extracellular vesicles (sEVs; popularly identified with the name “exosomes”) with a composite cargo of miRNAs are secreted by cultured... Read More about Bioinspired artificial exosomes based on lipid nanoparticles carrying let-7b-5p promote angiogenesis in vitro and in vivo.

Blocking endothelial apoptosis revascularises the retina in a model of ischemic retinopathy (2020)
Journal Article
Grant, Z. L., Whitehead, L., Wong, V. H. Y., He, Z., Yan, R. Y., Miles, A. R., Benest, A. V., Bates, D. O., Prahst, C., Bentley, K., Bui, B. V., Symons, R. C., & Coultas, L. (2020). Blocking endothelial apoptosis revascularises the retina in a model of ischemic retinopathy. Journal of Clinical Investigation, 130(8), 4235-4251. https://doi.org/10.1172/jci127668

Aberrant, neovascular retinal blood vessel growth is a vision-threatening complication in ischemic retinal diseases. It is driven by retinal hypoxia frequently caused by capillary nonperfusion and endothelial cell (EC) loss. We investigated the role... Read More about Blocking endothelial apoptosis revascularises the retina in a model of ischemic retinopathy.

Enhanced notch signaling modulates unproductive revascularization in response to nitric oxide-angiopoietin signaling in a mouse model of peripheral ischemia (2019)
Journal Article
Machado, M. J., Boardman, R., Riu, F., Emanueli, C., Benest, A. V., & Bates, D. O. (2019). Enhanced notch signaling modulates unproductive revascularization in response to nitric oxide-angiopoietin signaling in a mouse model of peripheral ischemia. Microcirculation, 26(6), Article e12549. https://doi.org/10.1111/micc.12549

Introduction
Arteriolargenesis can be induced by concomitant stimulation of nitric Oxide (NO)‐Angiopoietin receptor (Tie)‐Vascular Endothelial Growth Factor (VEGF) signaling in the rat mesentery angiogenesis assay. We hypothesized that the same comb... Read More about Enhanced notch signaling modulates unproductive revascularization in response to nitric oxide-angiopoietin signaling in a mouse model of peripheral ischemia.

BDNF (Brain-Derived Neurotrophic Factor) Promotes Embryonic Stem Cells Differentiation to Endothelial Cells Via a Molecular Pathway, Including MicroRNA-214, EZH2 (Enhancer of Zeste Homolog 2), and eNOS (Endothelial Nitric Oxide Synthase) (2018)
Journal Article
Descamps, B., Saif, J., Benest, A. V., Biglino, G., Bates, D. O., Chamorro-Jorganes, A., & Emanueli, C. (2018). BDNF (Brain-Derived Neurotrophic Factor) Promotes Embryonic Stem Cells Differentiation to Endothelial Cells Via a Molecular Pathway, Including MicroRNA-214, EZH2 (Enhancer of Zeste Homolog 2), and eNOS (Endothelial Nitric Oxide Synthase). Arteriosclerosis, Thrombosis, and Vascular Biology, 38(9), 2117-2125. https://doi.org/10.1161/atvbaha.118.311400

Can the co-dependence of the immune system and angiogenesis facilitate pharmacological targeting of tumours? (2017)
Journal Article
Mortara, L., Benest, A. V., Bates, D. O., & Noonan, D. M. (2017). Can the co-dependence of the immune system and angiogenesis facilitate pharmacological targeting of tumours?. Current Opinion in Pharmacology, 35, 66-74. https://doi.org/10.1016/j.coph.2017.05.009

Tumours elicit a number of mechanisms to induce a reprogramming of innate and adaptive immune cells to their advantage, inducing a pro-angiogenic phenotype. Investigation of these events is now leading to the identification of specific myeloid and ly... Read More about Can the co-dependence of the immune system and angiogenesis facilitate pharmacological targeting of tumours?.

The Orphan Receptor Tie1 Controls Angiogenesis and Vascular Remodeling by Differentially Regulating Tie2 in Tip and Stalk Cells (2015)
Journal Article
Savant, S., La Porta, S., Budnik, A., Busch, K., Hu, J., Tisch, N., Korn, C., Valls, A., Benest, A., Terhardt, D., Qu, X., Adams, R., Baldwin, H., Ruiz de Almodóvar, C., Rodewald, H.-R., & Augustin, H. (2015). The Orphan Receptor Tie1 Controls Angiogenesis and Vascular Remodeling by Differentially Regulating Tie2 in Tip and Stalk Cells. Cell Reports, 12(11), 1761-1773. https://doi.org/10.1016/j.celrep.2015.08.024