Outputs (13)

Potential of mesenchymal stem cells as topical immunomodulatory cell therapies for ocular surface inflammatory disorders (2020)
Journal Article
Beeken, L. J., Ting, D. S. J., & Sidney, L. E. (2020). Potential of mesenchymal stem cells as topical immunomodulatory cell therapies for ocular surface inflammatory disorders. Stem Cells Translational Medicine, 10(1), 39-49. https://doi.org/10.1002/sctm.20-0118

Ocular surface inflammatory disorders (OSIDs) are a group of highly prevalent, heterogeneous diseases which display a variety of aetiologies and symptoms and are risk factors for serious complications including ocular and cornea impairment. Corneal i... Read More about Potential of mesenchymal stem cells as topical immunomodulatory cell therapies for ocular surface inflammatory disorders.

The electrospinning of a thermo-responsive polymer with peptide conjugates for phenotype support and extracellular matrix production of therapeutically relevant mammalian cells (2020)
Journal Article
Ruiter, F. A., Sidney, L. E., Kiick, K. L., Segal, J. I., Alexander, C., & Rose, F. R. A. J. (2020). The electrospinning of a thermo-responsive polymer with peptide conjugates for phenotype support and extracellular matrix production of therapeutically relevant mammalian cells. Biomaterials Science, 8(9), 2611-2626. https://doi.org/10.1039/c9bm01965k

Current cell expansion methods for tissue engineering and regenerative medicine applications rely on the use of enzymatic digestion passaging and 2D platforms. However, this enzymatic treatment significantly reduces cell quality, due to the destructi... Read More about The electrospinning of a thermo-responsive polymer with peptide conjugates for phenotype support and extracellular matrix production of therapeutically relevant mammalian cells.

Validation and assessment of an antibiotic-based, aseptic decontamination manufacturing protocol for therapeutic, vacuum-dried human amniotic membrane (2019)
Journal Article
Marsit, N. M., Sidney, L. E., Britchford, E. R., McIntosh, O. D., Allen, C. L., Ashraf, W., …Hopkinson, A. (2019). Validation and assessment of an antibiotic-based, aseptic decontamination manufacturing protocol for therapeutic, vacuum-dried human amniotic membrane. Scientific Reports, 9(1), Article 12854. https://doi.org/10.1038/s41598-019-49314-7

© 2019, The Author(s). Amniotic membrane (AM) is used to treat a range of ophthalmic indications but must be presented in a non-contaminated state. AM from elective caesarean sections contains natural microbial contamination, requiring removal during... Read More about Validation and assessment of an antibiotic-based, aseptic decontamination manufacturing protocol for therapeutic, vacuum-dried human amniotic membrane.

Anti-inflammatory potential of human corneal stroma-derived stem cells determined by a novel in vitro corneal epithelial injury model (2019)
Journal Article
Morales, M. L. O., Marsit, N. M., McIntosh, O. D., Hopkinson, A., & Sidney, L. E. (2019). Anti-inflammatory potential of human corneal stroma-derived stem cells determined by a novel in vitro corneal epithelial injury model. World Journal of Stem Cells, 11(2), 84-99. https://doi.org/10.4252/wjsc.v11.i2.84

BACKGROUND An in vitro injury model mimicking a corneal surface injury was optimised using human corneal epithelial cells (hCEC). AIM To investigate whether corneal-stroma derived stem cells (CSSC) seeded on an amniotic membrane (AM) construct m... Read More about Anti-inflammatory potential of human corneal stroma-derived stem cells determined by a novel in vitro corneal epithelial injury model.

A thermoresponsive three-dimensional fibrous cell culture platform for enzyme-free expansion of mammalian cells (2019)
Journal Article
Aladdad, A. M., Amer, M. H., Sidney, L., Hopkinson, A., White, L. J., Alexander, C., & Rose, F. R. (2019). A thermoresponsive three-dimensional fibrous cell culture platform for enzyme-free expansion of mammalian cells. Acta Biomaterialia, 95, 427-438. https://doi.org/10.1016/j.actbio.2019.01.037

A three-dimensional thermoresponsive fibrous scaffold system for the subsequent extended culture and enzyme-free passaging of a range of mammalian cell types is presented. Poly(PEGMA188) was incorporated with poly(ethylene terephthalate) (PET) via bl... Read More about A thermoresponsive three-dimensional fibrous cell culture platform for enzyme-free expansion of mammalian cells.

In vitro evaluation of electrospun blends of gelatin and PCL for application as a partial thickness corneal graft (2018)
Journal Article
Rose, J., Sidney, L., Patient, J., White, L., Dua, H., El Haj, A., …Rose, F. (2018). In vitro evaluation of electrospun blends of gelatin and PCL for application as a partial thickness corneal graft. Journal of Biomedical Materials Research Part A, 107(4), 828-838. https://doi.org/10.1002/jbm.a.36598

The advent of innovative surgical procedures utilizing partial thickness corneal grafts has created a need for the development of synthetic implants to recreate corneal stromal tissue. This work evaluates electrospun gelatin and polycaprolactone (PCL... Read More about In vitro evaluation of electrospun blends of gelatin and PCL for application as a partial thickness corneal graft.

Terminal sterilization: conventional methods versus emerging cold atmospheric pressure plasma technology for non-viable biological tissues (2016)
Journal Article
Marsit, N. M., Sidney, L. E., Branch, M. J., Wilson, S. L., & Hopkinson, A. (in press). Terminal sterilization: conventional methods versus emerging cold atmospheric pressure plasma technology for non-viable biological tissues. Plasma Processes and Polymers, https://doi.org/10.1002/ppap.201600134

Tissue products are susceptible to microbial contamination from different sources, which may cause disease transmission upon transplantation. Terminal sterilization using gamma radiation, electron-beam, and ethylene oxide protocols are well-establish... Read More about Terminal sterilization: conventional methods versus emerging cold atmospheric pressure plasma technology for non-viable biological tissues.

Corneal keratocyte transition to mesenchymal stem cell phenotype and reversal using serum-free medium supplemented with FGF-2, TGF-ß3 and retinoic acid (2016)
Journal Article
Sidney, L. E., & Hopkinson, A. (in press). Corneal keratocyte transition to mesenchymal stem cell phenotype and reversal using serum-free medium supplemented with FGF-2, TGF-ß3 and retinoic acid. Journal of Tissue Engineering and Regenerative Medicine, https://doi.org/10.1002/term.2316

Keratocytes of the corneal limbal stroma can derive populations of mesenchymal stem cells (MSC) when expanded in vitro. However, once a corneal MSC (cMSC) phenotype is achieved, regaining the keratocyte phenotype can be challenging, and there is no s... Read More about Corneal keratocyte transition to mesenchymal stem cell phenotype and reversal using serum-free medium supplemented with FGF-2, TGF-ß3 and retinoic acid.

Effect of culture medium on propagation and phenotype of corneal stroma-derived stem cells (2015)
Journal Article
Sidney, L. E., Branch, M. J., Dua, H. S., & Hopkinson, A. (2015). Effect of culture medium on propagation and phenotype of corneal stroma-derived stem cells. Cytotherapy, 17(12), https://doi.org/10.1016/j.jcyt.2015.08.003

Background: The limbal area of the corneal stroma has been identified as a source of mesenchymal-like stem cells, which have potential for exploitation as a cell therapy. However, the optimal culture conditions are disputed and few direct media compa... Read More about Effect of culture medium on propagation and phenotype of corneal stroma-derived stem cells.

Corneal Decellularization: A Method of Recycling Unsuitable Donor Tissue for Clinical Translation? (2015)
Journal Article
Wilson, S. L., Sidney, L. E., Dunphy, S. E., Dua, H. S., & Hopkinson, A. (2016). Corneal Decellularization: A Method of Recycling Unsuitable Donor Tissue for Clinical Translation?. Current Eye Research, 41(6), 769-782. https://doi.org/10.3109/02713683.2015.1062114

Background: There is a clinical need for biomimetic corneas that are as effective, preferably superior, to cadaveric donor tissue. Decellularized tissues are advantageous compared to synthetic or semi-synthetic engineered tissues in that the native m... Read More about Corneal Decellularization: A Method of Recycling Unsuitable Donor Tissue for Clinical Translation?.