Outputs (54)

Assessing the potential of using the Langdon 5D(5B) substitution line for the introgression of Aegilops tauschii into durum wheat (2022)
Journal Article
Othmeni, M., Grewal, S., Walker, J., Yang, C., King, I. P., & King, J. (2022). Assessing the potential of using the Langdon 5D(5B) substitution line for the introgression of Aegilops tauschii into durum wheat. Frontiers in Plant Science, 13, Article 927728. https://doi.org/10.3389/fpls.2022.927728

Aegilops tauschii, the D-genome donor of hexaploid wheat, provides a source of genetic variation that could be used for tetraploid (durum) wheat improvement. In addition to the genes for wheat quality on the D-genome, which differentiate between brea... Read More about Assessing the potential of using the Langdon 5D(5B) substitution line for the introgression of Aegilops tauschii into durum wheat.

Introgression of the Triticum timopheevii Genome Into Wheat Detected by Chromosome-Specific Kompetitive Allele Specific PCR Markers (2022)
Journal Article
King, J., Grewal, S., Othmeni, M., Coombes, B., Yang, C., Walter, N., …King, I. (2022). Introgression of the Triticum timopheevii Genome Into Wheat Detected by Chromosome-Specific Kompetitive Allele Specific PCR Markers. Frontiers in Plant Science, 13, Article 919519. https://doi.org/10.3389/fpls.2022.919519

Triticum timopheevii (2n = 28, AtAtGG) is a tetraploid wild relative species with great potential to increase the genetic diversity of hexaploid wheat Triticum aestivum (2n = 42, AABBDD) for various important agronomic traits. A breeding scheme that... Read More about Introgression of the Triticum timopheevii Genome Into Wheat Detected by Chromosome-Specific Kompetitive Allele Specific PCR Markers.

Identifying variation for N-use efficiency and associated traits in amphidiploids derived from hybrids of bread wheat and the genera Aegilops, Secale, Thinopyrum and Triticum (2022)
Journal Article
Nehe, A., King, J., King, I. P., Murchie, E. H., & Foulkes, M. J. (2022). Identifying variation for N-use efficiency and associated traits in amphidiploids derived from hybrids of bread wheat and the genera Aegilops, Secale, Thinopyrum and Triticum. PLoS ONE, 17(4 April), Article e0266924. https://doi.org/10.1371/journal.pone.0266924

Future genetic progress in wheat grain yield will depend on increasing biomass and this must be achieved without commensurate increases in nitrogen (N) fertilizer inputs to minimize environmental impacts. In recent decades there has been a loss of ge... Read More about Identifying variation for N-use efficiency and associated traits in amphidiploids derived from hybrids of bread wheat and the genera Aegilops, Secale, Thinopyrum and Triticum.

Chromosome-specific KASP markers for detecting Amblyopyrum muticum segments in wheat introgression lines (2022)
Journal Article
Grewal, S., Coombes, B., Joynson, R., Hall, A., Fellers, J., Yang, C. Y., …King, J. (2022). Chromosome-specific KASP markers for detecting Amblyopyrum muticum segments in wheat introgression lines. Plant Genome, The, 15(1), Article e20193. https://doi.org/10.1002/tpg2.20193

Many wild-relative species are being used in prebreeding programs to increase the genetic diversity of wheat (Triticum aestivum L.). Genotyping tools such as single nucleotide polymorphism (SNP)-based arrays and molecular markers have been widely use... Read More about Chromosome-specific KASP markers for detecting Amblyopyrum muticum segments in wheat introgression lines.

Generation of Doubled Haploid Wheat-Triticum urartu Introgression Lines and Their Characterisation Using Chromosome-Specific KASP Markers (2021)
Journal Article
Grewal, S., Guwela, V., Newell, C., Yang, C. Y., Ashling, S., Scholefield, D., …King, J. (2021). Generation of Doubled Haploid Wheat-Triticum urartu Introgression Lines and Their Characterisation Using Chromosome-Specific KASP Markers. Frontiers in Plant Science, 12, Article 643636. https://doi.org/10.3389/fpls.2021.643636

Wheat is one of the most important food and protein sources in the world and although, in recent years wheat breeders have achieved yield gains, they are not sufficient to meet the demands of an ever-growing population. Development of high yielding w... Read More about Generation of Doubled Haploid Wheat-Triticum urartu Introgression Lines and Their Characterisation Using Chromosome-Specific KASP Markers.

Novel sources of variation in grain yield, components and mineral traits identified in wheat amphidiploids derived from thinopyrum bessarabicum (Savul. & rayss) Á. löve (poaceae) under saline soils in India (2020)
Journal Article
Khokhar, J. S., Sareen, S., Tyagi, B. S., Wilson, L., Young, S., King, J., …Broadley, M. R. (2020). Novel sources of variation in grain yield, components and mineral traits identified in wheat amphidiploids derived from thinopyrum bessarabicum (Savul. & rayss) Á. löve (poaceae) under saline soils in India. Sustainability, 12(21), Article 8975. https://doi.org/10.3390/su12218975

© 2020 by the authors. Licensee MDPI, Basel, Switzerland. Salt-affected soils constrain wheat production globally. A wild wheat species, Thinopyrum bessarabicum (Savul. & Rayss) Á. Löve (Poaceae), and its derivatives are tolerant of high external NaC... Read More about Novel sources of variation in grain yield, components and mineral traits identified in wheat amphidiploids derived from thinopyrum bessarabicum (Savul. & rayss) Á. löve (poaceae) under saline soils in India.

Variation in key leaf photosynthetic traits across wheat wild relatives is accession dependent not species dependent (2020)
Journal Article
McAusland, L., Vialet‐Chabrand, S., Jauregui, I., Burridge, A., Hubbart-Edwards, S., Fryer, M. J., …Murchie, E. H. (2020). Variation in key leaf photosynthetic traits across wheat wild relatives is accession dependent not species dependent. New Phytologist, 228(6), 1767-1780. https://doi.org/10.1111/nph.16832

©2020 The Authors. New Phytologist ©2020 New Phytologist Trust The wild relatives of modern wheat represent an underutilized source of genetic and phenotypic diversity and are of interest in breeding owing to their wide adaptation to diverse environm... Read More about Variation in key leaf photosynthetic traits across wheat wild relatives is accession dependent not species dependent.

Development of Wheat-Aegilops caudata Introgression Lines and Their Characterization Using Genome-Specific KASP Markers (2020)
Journal Article
Grewal, S., Othmeni, M., Walker, J., Hubbart Edwards, S., Yang, C. Y., Scholefield, D., …King, J. (2020). Development of Wheat-Aegilops caudata Introgression Lines and Their Characterization Using Genome-Specific KASP Markers. Frontiers in Plant Science, 11, Article 606. https://doi.org/10.3389/fpls.2020.00606

© Copyright © 2020 Grewal, Othmeni, Walker, Hubbart-Edwards, Yang, Scholefield, Ashling, Isaac, King and King. Aegilops caudata L. [syn. Ae. markgrafii (Greuter) Hammer], is a diploid wild relative of wheat (2n = 2x = 14, CC) and a valuable source fo... Read More about Development of Wheat-Aegilops caudata Introgression Lines and Their Characterization Using Genome-Specific KASP Markers.

Exploiting the genome of Thinopyrum elongatum to expand the gene pool of hexaploid wheat (2020)
Journal Article
Baker, L., Grewal, S., Yang, C. Y., Hubbart-Edwards, S., Scholefield, D., Ashling, S., …King, J. (2020). Exploiting the genome of Thinopyrum elongatum to expand the gene pool of hexaploid wheat. TAG Theoretical and Applied Genetics, 133, 2213–2226. https://doi.org/10.1007/s00122-020-03591-3

© 2020, The Author(s). Key message: One hundred and thirty four introgressions from Thinopyrum elongatum have been transferred into a wheat background and were characterised using 263 SNP markers. Abstract: Species within the genus Thinopyrum have be... Read More about Exploiting the genome of Thinopyrum elongatum to expand the gene pool of hexaploid wheat.

Identification of wheat cultivars for low nitrogen tolerance using multivariable screening approaches (2020)
Journal Article
Tyagi, B. S., Foulkes, J., Singh, G., Sareen, S., Kumar, P., Broadley, M. R., …Singh, G. P. (2020). Identification of wheat cultivars for low nitrogen tolerance using multivariable screening approaches. Agronomy, 10(3), 1-17. https://doi.org/10.3390/agronomy10030417

© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). A set of th... Read More about Identification of wheat cultivars for low nitrogen tolerance using multivariable screening approaches.

Novel sources of variation in grain Zinc (Zn) concentration in bread wheat germplasm derived from Watkins landraces (2020)
Journal Article
Khokhar, J. S., King, J., King, I. P., Young, S. D., Foulkes, M. J., De Silva, J., …Broadley, M. R. (2020). Novel sources of variation in grain Zinc (Zn) concentration in bread wheat germplasm derived from Watkins landraces. PLoS ONE, 15(2), Article e0229107. https://doi.org/10.1371/journal.pone.0229107

A diverse panel of 245 wheat genotypes, derived from crosses between landraces from the Watkins collection representing global diversity in the early 20th century and the modern wheat cultivar Paragon, was grown at two field sites in the UK in 2015-1... Read More about Novel sources of variation in grain Zinc (Zn) concentration in bread wheat germplasm derived from Watkins landraces.

Resistance to wheat rusts identified in wheat/Amblyopyrum muticum chromosome introgressions (2020)
Journal Article
Fellers, J. P., Matthews, A., Fritz, A. K., Rouse, M. N., Grewal, S., Hubbart‐Edwards, S., …King, J. (2020). Resistance to wheat rusts identified in wheat/Amblyopyrum muticum chromosome introgressions. Crop Science, 60(4), 1957-1964. https://doi.org/10.1002/csc2.20120

© 2020 The Authors. Crop Science © 2020 Crop Science Society of America Wheat (Triticum aestivum L.) rusts are a worldwide production problem. Plant breeders have used genetic resistance to combat these fungi. However, single-gene resistance is rapid... Read More about Resistance to wheat rusts identified in wheat/Amblyopyrum muticum chromosome introgressions.

The use of pentaploid crosses for the introgression of Amblyopyrum muticum and D-genome chromosome segments into durum wheat (2019)
Journal Article
Othmeni, M., Grewal, S., Hubbart-Edwards, S., Yang, C., Scholefield, D., Ashling, S., …King, J. (2019). The use of pentaploid crosses for the introgression of Amblyopyrum muticum and D-genome chromosome segments into durum wheat. Frontiers in Plant Science, 10, Article 1110. https://doi.org/10.3389/fpls.2019.01110

The wild relatives of wheat provide an important source of genetic variation for wheat improvement. Much of the work in the past aimed at transferring genetic variation from wild relatives into wheat has relied on the exploitation of the ph1b mutant,... Read More about The use of pentaploid crosses for the introgression of Amblyopyrum muticum and D-genome chromosome segments into durum wheat.

Rapid identification of homozygosity and site of wild relative introgressions in wheat through chromosome-specific KASP genotyping assays (2019)
Journal Article
Grewal, S., Hubbart-Edwards, S., Yang, C., Devi, U., Baker, L., Heath, J., …King, J. (2020). Rapid identification of homozygosity and site of wild relative introgressions in wheat through chromosome-specific KASP genotyping assays. Plant Biotechnology Journal, 18(3), 743-755. https://doi.org/10.1111/pbi.13241

For future food security, it is important that wheat, one of the most widely consumed crops in the world, can survive the threat of abiotic and biotic stresses. New genetic variation is currently being introduced into wheat through introgressions fro... Read More about Rapid identification of homozygosity and site of wild relative introgressions in wheat through chromosome-specific KASP genotyping assays.

Development of a New A –Genome-Specific Single Nucleotide Polymorphism Marker Set for the Molecular Characterization of Wheat– Introgression Lines (2019)
Journal Article
Cseh, A., Megyeri, M., Hubbart-Edwards, S., Yang, C., Scholefield, D., Ashling, S. S., …Grewal, S. (2019). Development of a New A –Genome-Specific Single Nucleotide Polymorphism Marker Set for the Molecular Characterization of Wheat– Introgression Lines. Plant Genome, The, 12(3), 1-7. https://doi.org/10.3835/plantgenome2018.12.0098

© 2019 The Author(s). Cultivated einkorn wheat (Triticum monococcum L. subsp. monococcum, 2n = 2x = 14, AmAm) and its wild relative T. monococcum subsp. aegilopoides are important sources of economically useful genes that can be exploited for wheat (... Read More about Development of a New A –Genome-Specific Single Nucleotide Polymorphism Marker Set for the Molecular Characterization of Wheat– Introgression Lines.

Juvenile root traits show limited correlation with grain yield, yield components and grain mineral composition traits in Indian wheat under hostile soils (2019)
Journal Article
Khokhar, J. S., Sareen, S., Tyagi, B. S., Wilson, L., King, I. P., Young, S. D., & Broadley, M. R. (2019). Juvenile root traits show limited correlation with grain yield, yield components and grain mineral composition traits in Indian wheat under hostile soils. Cereal Research Communications, 47(2), 369–382. https://doi.org/10.1556/0806.47.2019.10

© 2019 The Author(s) Correlations between juvenile wheat root traits, and grain yield and yield component traits under optimal field conditions have previously been reported in some conditions. The aim of this study was to test the hypothesis that ju... Read More about Juvenile root traits show limited correlation with grain yield, yield components and grain mineral composition traits in Indian wheat under hostile soils.

Development and characterisation of interspecific hybrid lines with genome-wide introgressions from Triticum timopheevii in a hexaploid wheat background (2019)
Journal Article
Devi, U., Grewal, S., Yang, C., Hubbart-Edwards, S., Scholefield, D., Ashling, S., …King, J. (2019). Development and characterisation of interspecific hybrid lines with genome-wide introgressions from Triticum timopheevii in a hexaploid wheat background. BMC Plant Biology, 19, https://doi.org/10.1186/s12870-019-1785-z

© 2019 The Author(s). Background: Triticum timopheevii (2n = 4x = 28; AtAtGG), is an important source for new genetic variation for wheat improvement with genes for potential disease resistance and salt tolerance. By generating a range of interspecif... Read More about Development and characterisation of interspecific hybrid lines with genome-wide introgressions from Triticum timopheevii in a hexaploid wheat background.

Development and validation of an exome-based SNP marker set for identification of the St, Jr and Jvs genomes of Thinopyrym intermedium in a wheat background (2019)
Journal Article
Cseh, A., Yang, C., Hubbart-Edwards, S., Scholefield, D., Ashling, S. S., Burridge, A. J., …Grewal, S. (2019). Development and validation of an exome-based SNP marker set for identification of the St, Jr and Jvs genomes of Thinopyrym intermedium in a wheat background. TAG Theoretical and Applied Genetics, 132(5), 1555–1570. https://doi.org/10.1007/s00122-019-03300-9

Thinopyrum intermedium (2n = 6x = 42, StJrJvs) is one of the most promising reservoirs of useful genes including tolerance to abiotic stresses, perenniality and disease resistance not available in the cultivated bread wheat. The transfer of genetic d... Read More about Development and validation of an exome-based SNP marker set for identification of the St, Jr and Jvs genomes of Thinopyrym intermedium in a wheat background.

Development of stable homozygous wheat/Amblyopyrum muticum (Aegilops mutica) introgression lines and their cytogenetic and molecular characterization (2019)
Journal Article
King, J., Newell, C., Grewal, S., Hubbart-Edwards, S., Yang, C., Scholefield, D., …King, I. P. (2019). Development of stable homozygous wheat/Amblyopyrum muticum (Aegilops mutica) introgression lines and their cytogenetic and molecular characterization. Frontiers in Plant Science, 10, 1-10. https://doi.org/10.3389/fpls.2019.00034

Wheat is one of the world’s most important sources of food. However, due to its evolution its genetic base has narrowed, which is severely limiting the ability of breeders to develop new higher yielding varieties that can adapt to the changing enviro... Read More about Development of stable homozygous wheat/Amblyopyrum muticum (Aegilops mutica) introgression lines and their cytogenetic and molecular characterization.

Developing a high-throughput SNP-based marker system to facilitate the introgression of traits from Aegilops species into bread wheat (Triticum aestivum) (2019)
Journal Article
Przewieslik-Allen, A. M., Burridge, A. J., Wilkinson, P. A., Winfield, M. O., Shaw, D. S., McAusland, L., …Barker, G. L. (2019). Developing a high-throughput SNP-based marker system to facilitate the introgression of traits from Aegilops species into bread wheat (Triticum aestivum). Frontiers in Plant Science, 9, Article 1993. https://doi.org/10.3389/fpls.2018.01993

The genus Aegilops contains a diverse collection of wild species exhibiting variation in geographical distribution, ecological adaptation, ploidy and genome organization. Aegilops is the most closely related genus to Triticum which includes cultivate... Read More about Developing a high-throughput SNP-based marker system to facilitate the introgression of traits from Aegilops species into bread wheat (Triticum aestivum).

An investigation of genotype-phenotype association in a festulolium forage grass population containing genome-spanning Festuca pratensis chromosome segments in a Lolium perenne background (2018)
Journal Article
Harper, J., Gasior, D., Mathews, R., Thomas, A., Evans, C., King, J., …Armstead, I. (2018). An investigation of genotype-phenotype association in a festulolium forage grass population containing genome-spanning Festuca pratensis chromosome segments in a Lolium perenne background. PLoS ONE, 13(11), Article e0207412. https://doi.org/10.1371/journal.pone.0207412

Alien chromosome introgression is used for the transfer of beneficial traits in plant breeding. For temperate forage grasses, much of the work in this context has focused on species within the ryegrasses (Lolium spp.) and the closely related fescues... Read More about An investigation of genotype-phenotype association in a festulolium forage grass population containing genome-spanning Festuca pratensis chromosome segments in a Lolium perenne background.

Detection of T. urartu introgressions in wheat and development of a panel of interspecific introgression lines (2018)
Journal Article
Grewal, S., Edwards, S. H., Yang, C., Scholefield, D., Ashling, S., Burridge, A., …King, J. (2018). Detection of T. urartu introgressions in wheat and development of a panel of interspecific introgression lines. Frontiers in Plant Science, 9, https://doi.org/10.3389/fpls.2018.01565

Tritcum urartu (2n = 2x = 14, AuAu), the A genome donor of wheat, is an important source for new genetic variation for wheat improvement due to its high photosynthetic rate and disease resistance. By facilitating the generation of genome-wide introgr... Read More about Detection of T. urartu introgressions in wheat and development of a panel of interspecific introgression lines.

B chromosomes are associated with redistribution of genetic recombination towards lower-recombination chromosomal regions in perennial ryegrass (2018)
Journal Article
Harper, J., Phillips, D., Thomas, A., Gasior, D., Evans, C., Powell, W., …Armstead, I. (2018). B chromosomes are associated with redistribution of genetic recombination towards lower-recombination chromosomal regions in perennial ryegrass. Journal of Experimental Botany, 69(8), https://doi.org/10.1093/jxb/ery052

Supernumerary ‘B’ chromosomes are non-essential components of the genome present in a range of plant and animal species – including many grasses. Within diploid and polyploid ryegrass and fescue species, including the forage grass perennial ryegrass... Read More about B chromosomes are associated with redistribution of genetic recombination towards lower-recombination chromosomal regions in perennial ryegrass.

Variation in grain Zn concentration, and the grain ionome, in field-grown Indian wheat (2018)
Journal Article
Khokhar, J. S., Sareen, S., Tyagi, B. S., Singh, G., Wilson, L., King, I. P., …Broadley, M. R. (2018). Variation in grain Zn concentration, and the grain ionome, in field-grown Indian wheat. PLoS ONE, 13(1), Article e0192026. https://doi.org/10.1371/journal.pone.0192026

Wheat is an important dietary source of zinc (Zn) and other mineral elements in many countries. Dietary Zn deficiency is widespread, especially in developing countries, and breeding (genetic biofortification) through the HarvestPlus programme has rec... Read More about Variation in grain Zn concentration, and the grain ionome, in field-grown Indian wheat.

Introgression of Aegilops speltoides segements in Triticum aestivum and the effect of the gametocidal genes (2017)
Journal Article
King, J., Grewal, S., Yang, C., Hubbart Edwards, S., Scholefield, D., Ashling, S., …King, I. P. (2018). Introgression of Aegilops speltoides segements in Triticum aestivum and the effect of the gametocidal genes. Annals of Botany, 121(12), https://doi.org/10.1093/aob/mcx149

• Background and Aims Bread wheat (Triticum aestivum) has been through a severe genetic bottleneck as a result of its evolution and domestication. It is therefore essential that new sources of genetic variation are generated and utilized. This study... Read More about Introgression of Aegilops speltoides segements in Triticum aestivum and the effect of the gametocidal genes.

Characterisation of Thinopyrum bessarabicum chromosomes through genome-wide introgressions into wheat (2017)
Journal Article
Grewal, S., Yang, C., Hubbart Edwards, S., Scholefield, D., Ashling, S., Burridge, A., …King, J. (2018). Characterisation of Thinopyrum bessarabicum chromosomes through genome-wide introgressions into wheat. TAG Theoretical and Applied Genetics, 131(2), 389–406. https://doi.org/10.1007/s00122-017-3009-y

Thinopyrum bessarabicum (2n = 2x = 14, JJ) is an important source for new genetic variation for wheat improvement due to its salinity tolerance and disease resistance. Its practical utilisation in wheat improvement can be facilitated through developm... Read More about Characterisation of Thinopyrum bessarabicum chromosomes through genome-wide introgressions into wheat.

Characterising variation in wheat traits under hostile soil conditions in India (2017)
Journal Article
Khokhar, J. S., Sareen, S., Tyagi, B. S., Singh, G., Chowdhury, A. K., Dhar, T., …Broadley, M. R. (2017). Characterising variation in wheat traits under hostile soil conditions in India. PLoS ONE, 12(6), Article e0179208. https://doi.org/10.1371/journal.pone.0179208

Intensive crop breeding has increased wheat yields and production in India. Wheat improvement in India typically involves selecting yield and component traits under non-hostile soil conditions at regional scales. The aim of this study is to quantify... Read More about Characterising variation in wheat traits under hostile soil conditions in India.

Comparative mapping and targeted-capture sequencing of the gametocidal loci in Aegilops sharonensis (2017)
Journal Article
Grewal, S., Gardiner, L., Ndreca, B., Knight, E., Moore, G., King, I. P., & King, J. (2017). Comparative mapping and targeted-capture sequencing of the gametocidal loci in Aegilops sharonensis. Plant Genome, The, 10(2), https://doi.org/10.3835/plantgenome2016.09.0090

Gametocidal (Gc) chromosomes or elements in species such as Aegilops sharonensis are preferentially transmitted to the next generation through both the male and female gametes when introduced into wheat. Furthermore, any genes, e.g. genes that contro... Read More about Comparative mapping and targeted-capture sequencing of the gametocidal loci in Aegilops sharonensis.

A step change in the transfer of interspecific variation into wheat from Amblyopyrum muticum (2016)
Journal Article
King, J., Grewal, S., Yang, C., Hubbart, S., Scholefield, D., Ashling, S., …King, I. P. (2017). A step change in the transfer of interspecific variation into wheat from Amblyopyrum muticum. Plant Biotechnology Journal, 15(2), 217-226. https://doi.org/10.1111/pbi.12606

Despite some notable successes, only a fraction of the genetic variation available in wild relatives has been utilized to produce superior wheat varieties. This is as a direct result of the lack of availability of suitable high-throughput technologie... Read More about A step change in the transfer of interspecific variation into wheat from Amblyopyrum muticum.

High-density SNP genotyping array for hexaploid wheat and its secondary and tertiary gene pool (2015)
Journal Article
Winfield, M. O., King, I., Allen, A. M., Burridge, A. J., Barker, G. L. A., Benbow, H. R., …Edwards, K. J. (2016). High-density SNP genotyping array for hexaploid wheat and its secondary and tertiary gene pool. Plant Biotechnology Journal, 14(5), 1195-1206. https://doi.org/10.1111/pbi.12485

In wheat, a lack of genetic diversity between breeding lines has been recognized as a significant block to future yield increases. Species belonging to bread wheat's secondary and tertiary gene pools harbour a much greater level of genetic variabilit... Read More about High-density SNP genotyping array for hexaploid wheat and its secondary and tertiary gene pool.

Generation of amphidiploids from hybrids of wheat and related species from the genera Aegilops, Secale, Thinopyrum and Triticum as a source of genetic variation for wheat improvement (2015)
Journal Article
Nemeth, C., Yang, C., Kasprzak, P., Hubbart, S., Scholefield, D., Mehra, S., …King, J. (2015). Generation of amphidiploids from hybrids of wheat and related species from the genera Aegilops, Secale, Thinopyrum and Triticum as a source of genetic variation for wheat improvement. Genome, 58(2), 71-79. https://doi.org/10.1139/gen-2015-0002

We aim to improve diversity of domesticated wheat by transferring genetic variation for important target traits from related wild and cultivated grass species. The present study describes the development of F1 hybrids between wheat and related specie... Read More about Generation of amphidiploids from hybrids of wheat and related species from the genera Aegilops, Secale, Thinopyrum and Triticum as a source of genetic variation for wheat improvement.

Mapping the ‘breaker’ element of the gametocidal locus proximal to a block of sub-telomeric heterochromatin on the long arm of chromosome 4Ssh of Aegilops sharonensis (2015)
Journal Article
Knight, E., Binnie, A., Draeger, T., Moscou, M., Rey, M., Sucher, J., …Moore, G. (2015). Mapping the ‘breaker’ element of the gametocidal locus proximal to a block of sub-telomeric heterochromatin on the long arm of chromosome 4Ssh of Aegilops sharonensis. TAG Theoretical and Applied Genetics, 128(6), 1049-1059. https://doi.org/10.1007/s00122-015-2489-x

The production of alien chromosome addition lines allows the transfer of useful genetic variation into elite wheat varieties from related wild species. However, some wild relatives of wheat, particularly those within the Sitopsis section of the genus... Read More about Mapping the ‘breaker’ element of the gametocidal locus proximal to a block of sub-telomeric heterochromatin on the long arm of chromosome 4Ssh of Aegilops sharonensis.

Transmission Frequencies of Introgressed Festuca pratensis Chromosomes and Chromosome Segments in Lolium perenne (2013)
Journal Article
King, J., Armstead, I., Harper, J., & King, I. (2013). Transmission Frequencies of Introgressed Festuca pratensis Chromosomes and Chromosome Segments in Lolium perenne. Crop Science, 53(5), 1968-1973. doi:10.2135/cropsci2013.03.0209

The introgression of genetic variation from related species into crops provides an important route by which superior plant varieties can be produced. The primary aim of introgression involves the transfer of a small chromosome segment from a related... Read More about Transmission Frequencies of Introgressed Festuca pratensis Chromosomes and Chromosome Segments in Lolium perenne.

A DArT marker genetic map of perennial ryegrass (Lolium perenne L.) integrated with detailed comparative mapping information; comparison with existing DArT marker genetic maps of Lolium perenne, L. multiflorum and Festuca pratensis (2013)
Journal Article
King, J., Thomas, A., James, C., King, I., & Armstead, I. (2013). A DArT marker genetic map of perennial ryegrass (Lolium perenne L.) integrated with detailed comparative mapping information; comparison with existing DArT marker genetic maps of Lolium perenne, L. multiflorum and Festuca pratensis. BMC Genomics, 14(1), https://doi.org/10.1186/1471-2164-14-437

Background Ryegrasses and fescues (genera, Lolium and Festuca) are species of forage and turf grasses which are used widely in agricultural and amenity situations. They are classified within the sub-family Pooideae and so are closely related to Brac... Read More about A DArT marker genetic map of perennial ryegrass (Lolium perenne L.) integrated with detailed comparative mapping information; comparison with existing DArT marker genetic maps of Lolium perenne, L. multiflorum and Festuca pratensis.

A novel grass hybrid to reduce flood generation in temperate regions (2013)
Journal Article
Macleod, C. J. A., Humphreys, M. W., Whalley, W. R., Turner, L., Binley, A., Watts, C. W., …Haygarth, P. M. (2013). A novel grass hybrid to reduce flood generation in temperate regions. Scientific Reports, 3(1), 1-7. https://doi.org/10.1038/srep01683

We report on the evaluation of a novel grass hybrid that provides efficient forage production and could help mitigate flooding. Perennial ryegrass (Lolium perenne) is the grass species of choice for most farmers, but lacks resilience against extremes... Read More about A novel grass hybrid to reduce flood generation in temperate regions.

Exploitation of interspecific diversity for monocot crop improvement (2013)
Journal Article
King, J., Armstead, I., Harper, J., Ramsey, L., Snape, J., Waugh, R., …King, I. (2013). Exploitation of interspecific diversity for monocot crop improvement. Heredity, 110(5), 475-483. https://doi.org/10.1038/hdy.2012.116

In many cultivated crop species there is limited genetic variation available for the development of new higher yielding varieties adapted to climate change and sustainable farming practises. The distant relatives of crop species provide a vast and la... Read More about Exploitation of interspecific diversity for monocot crop improvement.

Alien introgression in the grasses Lolium perenne (perennial ryegrass) and Festuca pratensis (meadow fescue): The development of seven monosomic substitution lines and their molecular and cytological characterization (2011)
Journal Article
Harper, J., Armstead, I., Thomas, A., James, C., Gasior, D., Bisaga, M., …King, J. (2011). Alien introgression in the grasses Lolium perenne (perennial ryegrass) and Festuca pratensis (meadow fescue): The development of seven monosomic substitution lines and their molecular and cytological characterization. Annals of Botany, 107(8), 1313-1321. https://doi.org/10.1093/aob/mcr083

BACKGROUND AND AIMS: To address the issues associated with food security, environmental change and bioenergy in the context of crop plants, the production, identification and evaluation of novel plant phenotypes is fundamental. One of the major rout... Read More about Alien introgression in the grasses Lolium perenne (perennial ryegrass) and Festuca pratensis (meadow fescue): The development of seven monosomic substitution lines and their molecular and cytological characterization.

Development of intron-flanking EST markers for the Lolium/Festuca complex using rice genomic information (2009)
Journal Article
Tamura, K., Yonemaru, J., Hisano, H., Kanamori, H., King, J., King, I. P., …Yamada, T. (2009). Development of intron-flanking EST markers for the Lolium/Festuca complex using rice genomic information. TAG Theoretical and Applied Genetics, 118(8), 1549-1560. https://doi.org/10.1007/s00122-009-1003-8

DNA markers able to distinguish species or genera with high specificity are valuable in the identification of introgressed regions in interspecific or intergeneric hybrids. Intergeneric hybridization between the genera of Lolium and Festuca, leading... Read More about Development of intron-flanking EST markers for the Lolium/Festuca complex using rice genomic information.

The control of chlorophyll catabolism and the status of yellowing as a biomarker of leaf senescence (2008)
Journal Article
Ougham, H., Hörtensteiner, S., Armstead, I., Donnison, I., King, I., Thomas, H., & Mur, L. (2008). The control of chlorophyll catabolism and the status of yellowing as a biomarker of leaf senescence. Plant Biology, 10, 4-14. https://doi.org/10.1111/j.1438-8677.2008.00081.x

The pathway of chlorophyll catabolism during leaf senescence is known in a fair amount of biochemical and cell biological detail. In the last few years, genes encoding a number of the catabolic enzymes have been characterized, including the key ring‐... Read More about The control of chlorophyll catabolism and the status of yellowing as a biomarker of leaf senescence.

Development of a Genomic Microsatellite Library in Perennial Ryegrass (Lolium perenne) and its Use in Trait Mapping (2008)
Journal Article
King, J., Thorogood, D., Edwards, K. J., Armstead, I. P., Roberts, L., Skot, K., …King, I. P. (in press). Development of a Genomic Microsatellite Library in Perennial Ryegrass (Lolium perenne) and its Use in Trait Mapping. Annals of Botany, 101(6), 845-853. https://doi.org/10.1093/aob/mcn016

Background and Aims Perennial ryegrass (Lolium perenne) is one of the key forage and amenity grasses throughout the world. In the UK it accounts for 70 % of all agricultural land use with an estimated farm gate value of £6 billion per annum. However... Read More about Development of a Genomic Microsatellite Library in Perennial Ryegrass (Lolium perenne) and its Use in Trait Mapping.

Identifying genetic components controlling fertility in the outcrossing grass species perennial ryegrass (Lolium perenne) by quantitative trait loci analysis and comparative genetics (2008)
Journal Article
Armstead, I. P., Turner, L. B., Marshall, A. H., Humphreys, M. O., King, I. P., & Thorogood, D. (2008). Identifying genetic components controlling fertility in the outcrossing grass species perennial ryegrass (Lolium perenne) by quantitative trait loci analysis and comparative genetics. New Phytologist, 178(3), 559-571. doi:10.1111/j.1469-8137.2008.02413.x

Mutational load and resource allocation factors and their effects on limiting seed set were investigated in ryegrass by comparative mapping genomics and quantitative trait loci (QTL) analysis in two perennial ryegrass (Lolium perenne) mapping familie... Read More about Identifying genetic components controlling fertility in the outcrossing grass species perennial ryegrass (Lolium perenne) by quantitative trait loci analysis and comparative genetics.

Rice pseudomolecule-anchored cross-species DNA sequence alignments indicate regional genomic variation in expressed sequence conservation (2007)
Journal Article
Armstead, I., Huang, L., King, J., Ougham, H., Thomas, H., & King, I. (2007). Rice pseudomolecule-anchored cross-species DNA sequence alignments indicate regional genomic variation in expressed sequence conservation. BMC Genomics, 8(1), Article 283. https://doi.org/10.1186/1471-2164-8-283

Background Various methods have been developed to explore inter-genomic relationships among plant species. Here, we present a sequence similarity analysis based upon comparison of transcript-assembly and methylation-filtered databases from five plan... Read More about Rice pseudomolecule-anchored cross-species DNA sequence alignments indicate regional genomic variation in expressed sequence conservation.

Comparative Analyses Between Lolium/Festuca Introgression Lines and Rice Reveal the Major Fraction of Functionally Annotated Gene Models Is Located in Recombination-Poor/Very Recombination-Poor Regions of the Genome (2007)
Journal Article
King, J., Armstead, I., Donnison, S., Roberts, L., Harper, J., Skot, K., …King, I. (2007). Comparative Analyses Between Lolium/Festuca Introgression Lines and Rice Reveal the Major Fraction of Functionally Annotated Gene Models Is Located in Recombination-Poor/Very Recombination-Poor Regions of the Genome. Genetics, 177(1), 597-606. https://doi.org/10.1534/genetics.107.075515

Publication of the rice genome sequence has allowed an in-depth analysis of genome organization in a model monocot plant species. This has provided a powerful tool for genome analysis in large-genome unsequenced agriculturally important monocot speci... Read More about Comparative Analyses Between Lolium/Festuca Introgression Lines and Rice Reveal the Major Fraction of Functionally Annotated Gene Models Is Located in Recombination-Poor/Very Recombination-Poor Regions of the Genome.

From phenotype to genotype: whole tissue profiling for plant breeding (2007)
Journal Article
Goodacre, R., Roberts, L., Ellis, D. I., Thorogood, D., Reader, S. M., Ougham, H., & King, I. (2007). From phenotype to genotype: whole tissue profiling for plant breeding. Metabolomics, 3(4), 489-501. doi:10.1007/s11306-007-0062-6

Fourier transform infrared spectroscopy (FT-IR) was used to obtain ‘holistic’ metabolic fingerprints from a wide range of plants to differentiate species, population, single plant genotype, and chromosomal constitution differences. Sample preparation... Read More about From phenotype to genotype: whole tissue profiling for plant breeding.

Introgression mapping in the grasses (2007)
Journal Article
King, J., Armstead, I. P., Donnison, I. S., Harper, J. A., Roberts, L. A., Thomas, H., …King, I. P. (2007). Introgression mapping in the grasses. Chromosome Research, 15(1), 105-113. https://doi.org/10.1007/s10577-006-1103-0

The unique properties of Lolium/Festuca hybrids and their derivatives provide an ideal system for intergeneric introgression. At IGER a focus on the Lolium perenne/Festuca pratensis system is being exploited to elucidate genome organization in the gr... Read More about Introgression mapping in the grasses.

Cross-Species Identification of Mendel's I Locus (2007)
Journal Article
Armstead, I., Donnison, I., Aubry, S., Harper, J., Hortensteiner, S., James, C., …King, I. (2007). Cross-Species Identification of Mendel's I Locus. Science, 315(5808), 73-73. https://doi.org/10.1126/science.1132912

A key gene involved in plant senescence, mutations of which partially disable chlorophyll catabolism and confer stay-green leaf and cotyledon phenotypes, has been identified in Pisum sativum, Arabidopsis thaliana, and Festuca pratensis by using class... Read More about Cross-Species Identification of Mendel's I Locus.

Alignment of the Genomes of Brachypodium distachyon and Temperate Cereals and Grasses Using Bacterial Artificial Chromosome Landing With Fluorescence in Situ Hybridization (2006)
Journal Article
Hasterok, R., Marasek, A., Donnison, I. S., Armstead, I., Thomas, A., King, I. P., …Jenkins, G. (2006). Alignment of the Genomes of Brachypodium distachyon and Temperate Cereals and Grasses Using Bacterial Artificial Chromosome Landing With Fluorescence in Situ Hybridization. Genetics, 173(1), 349-362. https://doi.org/10.1534/genetics.105.049726

As part of an initiative to develop Brachypodium distachyon as a genomic “bridge” species between rice and the temperate cereals and grasses, a BAC library has been constructed for the two diploid (2n = 2x = 10) genotypes, ABR1 and ABR5. The library... Read More about Alignment of the Genomes of Brachypodium distachyon and Temperate Cereals and Grasses Using Bacterial Artificial Chromosome Landing With Fluorescence in Situ Hybridization.

Introgression of crown rust (Puccinia coronata) resistance from meadow fescue (Festuca pratensis) into Italian ryegrass (Lolium multiflorum): genetic mapping and identification of associated molecular markers (2005)
Journal Article
Armstead, I., Harper, J., Turner, L., Skot, L., King, I., Humphreys, M., …Roderick, H. (2006). Introgression of crown rust (Puccinia coronata) resistance from meadow fescue (Festuca pratensis) into Italian ryegrass (Lolium multiflorum): genetic mapping and identification of associated molecular markers. Plant Pathology, 55(1), 62-67. doi:10.1111/j.1365-3059.2005.01308.x

Identification of perennial ryegrass (Lolium perenne (L.)) and meadow fescue (Festuca pratensis (Huds.)) candidate orthologous sequences to the rice Hd1(Se1) and barley HvCO1 CONSTANS-like genes through comparative mapping and microsynteny (2005)
Journal Article
Armstead, I., Skøt, L., Turner, L., Skøt, K., Donnison, I., Humphreys, M., & King, I. (2005). Identification of perennial ryegrass (Lolium perenne (L.)) and meadow fescue (Festuca pratensis (Huds.)) candidate orthologous sequences to the rice Hd1(Se1) and barley HvCO1 CONSTANS-like genes through comparative mapping and microsynteny. New Phytologist, 167(1), 239-247. doi:10.1111/j.1469-8137.2005.01392.x

Microsynteny with rice and comparative genetic mapping were used to identify candidate orthologous sequences to the rice Hd1(Se1) gene in Lolium perenne and Festuca pratensis. A F. pratensis bacterial artificial chromosome (BAC) library was screened... Read More about Identification of perennial ryegrass (Lolium perenne (L.)) and meadow fescue (Festuca pratensis (Huds.)) candidate orthologous sequences to the rice Hd1(Se1) and barley HvCO1 CONSTANS-like genes through comparative mapping and microsynteny.

Construction of a Festuca pratensis BAC library for map-based cloning in Festulolium substitution lines (2005)
Journal Article
Donnison, I. S., O’Sullivan, D. M., Thomas, A., Canter, P., Moore, B., Armstead, I., …King, I. P. (2005). Construction of a Festuca pratensis BAC library for map-based cloning in Festulolium substitution lines. TAG Theoretical and Applied Genetics, 110(5), 846-851. doi:10.1007/s00122-004-1870-y

Introgression in Festulolium is a potentially powerful tool to isolate genes for a large number of traits which differ between Festuca pratensis Huds. and Lolium perenne L. Not only are hybrids between the two species fertile, but the two genomes can... Read More about Construction of a Festuca pratensis BAC library for map-based cloning in Festulolium substitution lines.

Molecular tagging of a senescence gene by introgression mapping of a stay-green mutation from Festuca pratensis (2004)
Journal Article
Moore, B. J., Donnison, I. S., Harper, J. A., Armstead, I. P., King, J., Thomas, H., …King, I. P. (2005). Molecular tagging of a senescence gene by introgression mapping of a stay-green mutation from Festuca pratensis. New Phytologist, 165(3), 801-806. https://doi.org/10.1111/j.1469-8137.2004.01269.x

Intergeneric hybrids between Lolium multiflorum and Festuca pratensis (Lm/Fp) and their derivatives exhibit a unique combination of genetic and cytogenetic characteristics: chromosomes undergo a high frequency of homoeologous recombination at meiosis... Read More about Molecular tagging of a senescence gene by introgression mapping of a stay-green mutation from Festuca pratensis.

Synteny between a major heading-date QTL in perennial ryegrass (Lolium perenne L.) and the Hd3 heading-date locus in rice (2003)
Journal Article
Armstead, I., Turner, L., Farrell, M., Skøt, L., Gomez, P., Montoya, T., …Humphreys, M. (2004). Synteny between a major heading-date QTL in perennial ryegrass (Lolium perenne L.) and the Hd3 heading-date locus in rice. TAG Theoretical and Applied Genetics, 108(5), 822-828. doi:10.1007/s00122-003-1495-6

The genetic control of induction to flowering has been studied extensively in both model and crop species because of its fundamental biological and economic significance. An ultimate aim of many of these studies has been the application of the unders... Read More about Synteny between a major heading-date QTL in perennial ryegrass (Lolium perenne L.) and the Hd3 heading-date locus in rice.

Comparison and integration of genetic maps generated from F2 and BC1-type mapping populations in perennial ryegrass (2002)
Journal Article
Armstead, I., Turner, L., King, I., Cairns, A., & Humphreys, M. (2002). Comparison and integration of genetic maps generated from F2 and BC1-type mapping populations in perennial ryegrass. Plant Breeding, 121(6), 501-507. doi:10.1046/j.1439-0523.2002.00742.x

Linkage maps of perennial ryegrass were constructed from F2 and BC1?type populations using, predominantly, restriction fragment length polymorphism data based on heterologous probes used in mapping other grass species. The maps identified seven linka... Read More about Comparison and integration of genetic maps generated from F2 and BC1-type mapping populations in perennial ryegrass.

A High-Copy-Number CACTA Family Transposon in Temperate Grasses and Cereals (2002)
Journal Article
Langdon, T., Jenkins, G., Hasterok, R., Jones, R. N., & King, I. P. (2003). A High-Copy-Number CACTA Family Transposon in Temperate Grasses and Cereals. Genetics, 163(3), 1097-1108

A lineage of CACTA family transposons has been identified in temperate grasses and cereals, and a full-length representative of the subfamily from Lolium perenne has been sequenced. Both the size and internal organization of the L. perenne element ar... Read More about A High-Copy-Number CACTA Family Transposon in Temperate Grasses and Cereals.