All Outputs (84)

Hsmar1 transposition is sensitive to the topology of the transposon donor and the target (2013)
Journal Article
Claeys Bouuaert, C., & Chalmers, R. (2013). Hsmar1 transposition is sensitive to the topology of the transposon donor and the target. PLoS ONE, 8(1), Article 0053690. https://doi.org/10.1371/journal.pone.0053690

Hsmar1 is a member of the Tc1-mariner superfamily of DNA transposons. These elements mobilize within the genome of their host by a cut-and-paste mechanism. We have exploited the in vitro reaction provided by Hsmar1 to investigate the effect of DNA su... Read More about Hsmar1 transposition is sensitive to the topology of the transposon donor and the target.

The extracellular signal-regulated kinase (ERK) pathway: a potential therapeutic target in hypertension (2012)
Journal Article
Roberts, R. E. (2012). The extracellular signal-regulated kinase (ERK) pathway: a potential therapeutic target in hypertension. Journal of Experimental Pharmacology, 4, https://doi.org/10.2147/JEP.S28907

Hypertension is a risk factor for myocardial infarction, stroke, renal failure, heart failure, and peripheral vascular disease. One feature of hypertension is a hyperresponsiveness to contractile agents, and inhibition of vasoconstriction forms the b... Read More about The extracellular signal-regulated kinase (ERK) pathway: a potential therapeutic target in hypertension.

Fluorescence correlation spectroscopy, combined with bimolecular fluorescence complementation, reveals the effects of ?-arrestin complexes and endocytic targeting on the membrane mobility of neuropeptide Y receptors (2012)
Journal Article
Kilpatrick, L. E., Briddon, S. J., & Holliday, N. D. (2012). Fluorescence correlation spectroscopy, combined with bimolecular fluorescence complementation, reveals the effects of ?-arrestin complexes and endocytic targeting on the membrane mobility of neuropeptide Y receptors. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1823(6),

Fluorescence correlation spectroscopy (FCS) and photon counting histogram (PCH) analysis are powerful ways to study mobility and stoichiometry of G protein coupled receptor complexes, within microdomains of single living cells. However, relating thes... Read More about Fluorescence correlation spectroscopy, combined with bimolecular fluorescence complementation, reveals the effects of ?-arrestin complexes and endocytic targeting on the membrane mobility of neuropeptide Y receptors.

In vitro and in vivo analysis of a novel highly selective ?1-adrenoceptor partial agonist. (2012)
Conference Proceeding
Baker, J. G., Mistry, S. N., Fischer, P. M., Hill, S. J., Gardiner, S. M., & Kellam, B. (2012). In vitro and in vivo analysis of a novel highly selective ?1-adrenoceptor partial agonist.

Fluoxetine counteracts the cognitive and cellular effects of 5-Fluorouracil in the rat hippocampus by a mechanism of prevention rather than recovery (2012)
Journal Article
Lyons, L., ELBeltagy, M., Bennett, G., & Wigmore, P. (2012). Fluoxetine counteracts the cognitive and cellular effects of 5-Fluorouracil in the rat hippocampus by a mechanism of prevention rather than recovery. PLoS ONE, 7(1), Article e30010. https://doi.org/10.1371/journal.pone.0030010

5-Fluorouracil (5-FU) is a cytostatic drug associated with chemotherapy-induced cognitive impairments that many cancer patients experience after treatment. Previous work in rodents has shown that 5-FU reduces hippocampal cell proliferation, a possi... Read More about Fluoxetine counteracts the cognitive and cellular effects of 5-Fluorouracil in the rat hippocampus by a mechanism of prevention rather than recovery.

Predicting in vivo cardiovascular properties of ?-blockers from cellular assays: a quantative comparison of cellular and cardiovascular pharmacological responses (2011)
Journal Article
Baker, J. G., Kemp, P., March, J., Fretwell, L., Hill, S. J., & Gardiner, S. M. (2011). Predicting in vivo cardiovascular properties of ?-blockers from cellular assays: a quantative comparison of cellular and cardiovascular pharmacological responses. FASEB Journal, 25(12), https://doi.org/10.1096/fj.11-192435

?-Adrenoceptor antagonists differ in their degree of partial agonism. In vitro assays have provided information on ligand affinity, selectivity, and intrinsic efficacy. However, the extent to which these properties are manifest in vivo is less clear.... Read More about Predicting in vivo cardiovascular properties of ?-blockers from cellular assays: a quantative comparison of cellular and cardiovascular pharmacological responses.

Dimerization of ABCG2 analysed by bimolecular fluorescence complementation (2011)
Journal Article
Haider, A. J., Briggs, D., Self, T. J., Chilvers, H. L., Holliday, N. D., & Kerr, I. D. (2011). Dimerization of ABCG2 analysed by bimolecular fluorescence complementation. PLoS ONE, 6(10), Article e25818. https://doi.org/10.1371/journal.pone.0025818

ABCG2 is one of three human ATP binding cassette transporters that are functionally capable of exporting a diverse range of substrates from cells. The physiological consequence of ABCG2 multidrug transport activity in leukaemia, and some solid tumour... Read More about Dimerization of ABCG2 analysed by bimolecular fluorescence complementation.

Knockdown of embryonic myosin heavy chain reveals an essential role in the morphology and function of the developing heart (2011)
Journal Article
Rutland, C. S., Polo-Parada, L., Ehler, E., Alibhai, A., Thorpe, A., Suren, S., …Loughna, S. (2011). Knockdown of embryonic myosin heavy chain reveals an essential role in the morphology and function of the developing heart. Development, 138(18), https://doi.org/10.1242/dev.059063

The expression and function of embryonic myosin heavy chain (eMYH) has not been investigated within the early developing heart. This is despite the knowledge that other structural proteins, such as alpha and beta myosin heavy chains and cardiac alpha... Read More about Knockdown of embryonic myosin heavy chain reveals an essential role in the morphology and function of the developing heart.

Functional network construction in Arabidopsis using rule-based machine learning on large-scale data sets (2011)
Journal Article
Bassel, G. W., Glaab, E., Marquez, J., Holdsworth, M. J., & Barcardit, J. (2011). Functional network construction in Arabidopsis using rule-based machine learning on large-scale data sets. Plant Cell, 23(9), https://doi.org/10.1105/tpc.111.088153

The meta-analysis of large-scale postgenomics data sets within public databases promises to provide important novel biological knowledge. Statistical approaches including correlation analyses in coexpression studies of gene expression have emerged as... Read More about Functional network construction in Arabidopsis using rule-based machine learning on large-scale data sets.

SUMO conjugation of STAT1 protects cells from hyperresponsiveness to IFN? (2011)
Journal Article
Begitt, A., Droescher, M., Knobeloch, K., & Vinkemeier, U. (2011). SUMO conjugation of STAT1 protects cells from hyperresponsiveness to IFN?. Blood, 118(4), https://doi.org/10.1182/blood-2011-04-347930

The biologic effects of IFNγ are mediated by the transcription factor STAT1. The activity of STAT1 is inhibited by small ubiquitin-like modifier (SUMO) conjugation. This occurs both directly through decreasing STAT1 tyrosine phosphorylation and indir... Read More about SUMO conjugation of STAT1 protects cells from hyperresponsiveness to IFN?.

STAT1 signaling is not regulated by a phosphorylation-acetylation switch (2011)
Journal Article
Antunes, F., Marg, A., & Vinkemeier, U. (2011). STAT1 signaling is not regulated by a phosphorylation-acetylation switch. Molecular and Cellular Biology, 31(14), https://doi.org/10.1128/MCB.05300-11

The treatment of cells with histone deacetylase inhibitors (HDACi) was reported to reveal the acetylation of STAT1 at lysine 410 and lysine 413 (O. H. Kra¨mer et al., Genes Dev. 20:473–485, 2006). STAT1 acetylation was proposed to regulate apoptosi... Read More about STAT1 signaling is not regulated by a phosphorylation-acetylation switch.

H-NS mediates the dissociation of a refractory protein–DNA complex during Tn10/IS10 transposition (2011)
Journal Article
Liu, D., Haniford, D. B., & Chalmers, R. (2011). H-NS mediates the dissociation of a refractory protein–DNA complex during Tn10/IS10 transposition. Nucleic Acids Research, 39(15), https://doi.org/10.1093/nar/gkr309

Tn10/IS10 transposition takes place in the context of a protein–DNA complex called a transpososome. During the reaction, the transpososome undergoes several conformational changes. The host proteins IHF and H-NS, which also are global regulators of g... Read More about H-NS mediates the dissociation of a refractory protein–DNA complex during Tn10/IS10 transposition.

Evolution of ?-blockers: from anti-anginal drugs to ligand-directed signalling (2011)
Journal Article
Baker, J. G., Hill, S. J., & Summers, R. J. (2011). Evolution of ?-blockers: from anti-anginal drugs to ligand-directed signalling. Trends in Pharmacological Sciences, 32(4), https://doi.org/10.1016/j.tips.2011.02.010

Sir James Black developed ?-blockers, one of the most useful groups of drugs in use today. Not only are they being used for their original purpose to treat angina and cardiac arrhythmias, but they are also effective therapeutics for hypertension, car... Read More about Evolution of ?-blockers: from anti-anginal drugs to ligand-directed signalling.

The transposon-like correia elements encode numerous strong promoters and provide a potential new mechanism for phase variation in the meningococcus (2011)
Journal Article
Siddique, A., Buisine, N., & Chalmers, R. (2011). The transposon-like correia elements encode numerous strong promoters and provide a potential new mechanism for phase variation in the meningococcus. PLoS Genetics, 7(1), Article 13. https://doi.org/10.1371/journal.pgen.1001277

Neisseria meningitidis is the primary causative agent of bacterial meningitis. The genome is rich in repetitive DNA and almost 2% is occupied by a diminutive transposon called the Correia element. Here we report a bioinformatic analysis defining eigh... Read More about The transposon-like correia elements encode numerous strong promoters and provide a potential new mechanism for phase variation in the meningococcus.

Nuclear survivin: cellular consequences and therapeutic implications (2011)
Book Chapter
Wheatley, S. P. (2011). Nuclear survivin: cellular consequences and therapeutic implications. In H. Gali-Muhtasib (Ed.), Advances in cancer therapy. InTech. https://doi.org/10.5772/24795

The great deluge algorithm explores neighbouring solutions which are accepted if they are better than the best solution so far or if the detriment in quality is no larger than the current water level. In the original great deluge method, the water le... Read More about Nuclear survivin: cellular consequences and therapeutic implications.

Tonic modulation of spinal hyperexcitability by the endocannabinoid receptor system in a rat model of osteoarthritis pain (2010)
Journal Article
Sagar, D. R., Staniaszek, L. E., Okine, B. N., Woodhams, S., Norris, L. M., Pearson, R. G., …Chapman, V. (2010). Tonic modulation of spinal hyperexcitability by the endocannabinoid receptor system in a rat model of osteoarthritis pain. Arthritis and Rheumatism, 62(12), https://doi.org/10.1002/art.27698

Objective. To investigate the impact of an experimental model of osteoarthritis (OA) on spinal nociceptive processing and the role of the inhibitory endocannabinoid system in regulating sensory processing at the spinal level. Methods. Experiment... Read More about Tonic modulation of spinal hyperexcitability by the endocannabinoid receptor system in a rat model of osteoarthritis pain.

Base flipping in Tn10 transposition: an active flip and capture mechanism (2009)
Journal Article
Bischerour, J., & Chalmers, R. (2009). Base flipping in Tn10 transposition: an active flip and capture mechanism. PLoS ONE, 4(7), Article e6201. https://doi.org/10.1371/journal.pone.0006201

The bacterial Tn5 and Tn10 transposases have a single active site that cuts both strands of DNA at their respective transposon ends. This is achieved using a hairpin intermediate that requires the DNA to change conformation during the reaction. In Tn... Read More about Base flipping in Tn10 transposition: an active flip and capture mechanism.

Sensory gating and its modulation by cannabinoids: electrophysiological, computational and mathematical analysis (2008)
Journal Article
Zachariou, M., Dissanayake, D. W. N., Coombes, S., Owen, M. R., & Mason, R. (in press). Sensory gating and its modulation by cannabinoids: electrophysiological, computational and mathematical analysis. Cognitive Neurodynamics, 2, https://doi.org/10.1007/s11571-008-9050-4

Gating of sensory information can be assessed using an auditory conditioning-test paradigm which measures the reduction in the auditory evoked response to a test stimulus following an initial conditioning stimulus. Recording brainwaves from specific... Read More about Sensory gating and its modulation by cannabinoids: electrophysiological, computational and mathematical analysis.

Elucidating the interactions between the adhesive and transcriptioanl functions of beta-catenin in normal and cancerous cells (2007)
Journal Article
van Leeuwen, I. M. M., Byrne, H. M., Jensen, O. E., & King, J. R. (2007). Elucidating the interactions between the adhesive and transcriptioanl functions of beta-catenin in normal and cancerous cells. Journal of Theoretical Biology, 247(1), 77-102. https://doi.org/10.1016/j.jtbi.2007.01.019

Wnt signalling is involved in a wide range of physiological and pathological processes. The presence of an extracellular Wnt stimulus induces cytoplasmic stabilisation and nuclear translocation of beta-catenin, a protein that also plays an essential... Read More about Elucidating the interactions between the adhesive and transcriptioanl functions of beta-catenin in normal and cancerous cells.

Analgesic Effects of Fatty Acid Amide Hydrolase Inhibition in a Rat Model of Neuropathic Pain (2006)
Journal Article
Jhaveri, M., Richardson, D., Kendall, D., Barrett, D., & Chapman, V. (2006). Analgesic Effects of Fatty Acid Amide Hydrolase Inhibition in a Rat Model of Neuropathic Pain

Cannabinoid-based medicines have therapeutic potential for the treatment of pain. Augmentation of levels of endocannabinoids with inhibitors of fatty acid amide hydrolase (FAAH) is analgesic in models of acute and inflammatory pain states. The aim of... Read More about Analgesic Effects of Fatty Acid Amide Hydrolase Inhibition in a Rat Model of Neuropathic Pain.