All Outputs (88)

Coinfection and Emergence of Rifamycin Resistance during a Recurrent Clostridium difficile Infection (2016)
Journal Article
Stevenson, E. C., Major, G. A., Spiller, R. C., Kuehne, S. A., & Minton, N. P. (2016). Coinfection and Emergence of Rifamycin Resistance during a Recurrent Clostridium difficile Infection. Journal of Clinical Microbiology, 54(11), 2689-2694. https://doi.org/10.1128/jcm.01025-16

Clostridium difficile (Peptoclostridium difficile) is a common health care-associated infection with a disproportionately high incidence in elderly patients. Disease symptoms range from mild diarrhea to life-threatening pseudomembranous colitis. Arou... Read More about Coinfection and Emergence of Rifamycin Resistance during a Recurrent Clostridium difficile Infection.

A genetic assay for gene essentiality in Clostridium (2016)
Journal Article
Walker, D. J., Heap, J. T., Winzer, K., & Minton, N. P. (2016). A genetic assay for gene essentiality in Clostridium. Anaerobe, 42, 40-43. https://doi.org/10.1016/j.anaerobe.2016.07.007

Essential genes of pathogens are potential therapeutic targets, but are difficult to verify. Here, gene essentiality was determined by targeted knockout following engineered gene duplication. Null mutants of candidate essential genes of Clostridium d... Read More about A genetic assay for gene essentiality in Clostridium.

The binary toxin CDT enhances Clostridium difficile virulence by suppressing protective colonic eosinophilia (2016)
Journal Article
Cowardin, C. A., Buonomo, E. L., Saleh, M. M., Wilson, M. G., Burgess, S. L., Schwan, C., …Petri, W. A. (2016). The binary toxin CDT enhances Clostridium difficile virulence by suppressing protective colonic eosinophilia. Nature Microbiology, 1(8), Article 16108. https://doi.org/10.1038/nmicrobiol.2016.108

© 2016 Macmillan Publishers Limited. All rights reserved. Clostridium difficile is the most common hospital acquired pathogen in the USA, and infection is, in many cases, fatal. Toxins A and B are its major virulence factors, but expression of a thir... Read More about The binary toxin CDT enhances Clostridium difficile virulence by suppressing protective colonic eosinophilia.

SBRC-Nottingham: sustainable routes to platform chemicals from C1 wastes Gases (2016)
Journal Article
Burbidge, A., & Minton, N. P. (2016). SBRC-Nottingham: sustainable routes to platform chemicals from C1 wastes Gases. Biochemical Society Transactions, 44(3), 684-686. https://doi.org/10.1042/BST20160010

SBRC-Nottingham (www.sbrc-nottingham.ac.uk) was one of the first three UK university-based Synthetic Biology Research Centres (SBRCs) to be funded by the BBSRC and EPSRC as part of the recommendations made in the UKs Synthetic Biology Roadmap. It was... Read More about SBRC-Nottingham: sustainable routes to platform chemicals from C1 wastes Gases.

Insights into CO2 fixation pathway of Clostridium autoethanogenumby targeted mutagenesis (2016)
Journal Article
Liew, F., Henstra, A. M., Winzer, K., Kopke, M., Simpson, S. D., & Minton, N. P. (in press). Insights into CO2 fixation pathway of Clostridium autoethanogenumby targeted mutagenesis. mBio, 7(3), Article e00427-16. https://doi.org/10.1128/mBio.00427-16

The future sustainable production of chemicals and fuels from nonpetrochemical resources and reduction of greenhouse gas emissions are two of the greatest societal challenges. Gas fermentation, which utilizes the ability of acetogenic bacteria such a... Read More about Insights into CO2 fixation pathway of Clostridium autoethanogenumby targeted mutagenesis.

A roadmap for gene system development in Clostridium (2016)
Journal Article
Minton, N. P., Ehsaan, M., Humphreys, C. M., Little, G. T., Baker, J., Henstra, A. M., …Zhang, Y. (2016). A roadmap for gene system development in Clostridium. Anaerobe, 41, 104-112. https://doi.org/10.1016/j.anaerobe.2016.05.011

Clostridium species are both heroes and villains. Some cause serious human and animal diseases, those present in the microbiota contribute to health and wellbeing, while others represent useful industrial chassis for the production of chemicals and f... Read More about A roadmap for gene system development in Clostridium.

CRISPR-based genome editing and expression control systems in Clostridium acetobutylicum and Clostridium beijerinckii (2016)
Journal Article
Li, Q., Cheng, J., Minton, N. P., Zhang, Y., Wen, Z., Liu, J., …Yang, S. (2016). CRISPR-based genome editing and expression control systems in Clostridium acetobutylicum and Clostridium beijerinckii. Biotechnology Journal, 11(7), 961-972. https://doi.org/10.1002/biot.201600053

Solventogenic clostridia are important industrial microorganisms that produce various chemicals and fuels. Effective genetic tools would facilitate physiological studies aimed both at improving our understanding of metabolism and optimizing solvent p... Read More about CRISPR-based genome editing and expression control systems in Clostridium acetobutylicum and Clostridium beijerinckii.

Production of a functional cell wall-anchored minicellulosome by recombinant Clostridium acetobutylicum ATCC 824 (2016)
Journal Article
Willson, B. J., Kovács, K., Wilding-Steele, T., Markus, R., Winzer, K., & Minton, N. P. (2016). Production of a functional cell wall-anchored minicellulosome by recombinant Clostridium acetobutylicum ATCC 824. Biotechnology for Biofuels, 9(1), Article 109. https://doi.org/10.1186/s13068-016-0526-x

Background: The use of fossil fuels is no longer tenable. Not only are they a finite resource, their use is damaging the environment through pollution and global warming. Alternative, environmentally friendly, renewable sources of chemicals and fuels... Read More about Production of a functional cell wall-anchored minicellulosome by recombinant Clostridium acetobutylicum ATCC 824.

Development of an inducible transposon system for efficient random mutagenesis in Clostridium acetobutylicum (2016)
Journal Article
Zhang, Y., Xu, S., Chai, C., Yang, S., Jiang, W., Minton, N. P., & Gu, Y. (2016). Development of an inducible transposon system for efficient random mutagenesis in Clostridium acetobutylicum. FEMS Microbiology Letters, 363(8), Article fnw065. https://doi.org/10.1093/femsle/fnw065

Clostridium acetobutylicum is an industrially important Gram-positive organism which is capable of producing economically important chemicals in the ABE (Acetone, Butanol and Ethanol) fermentation process. Renewed interests in the ABE process necessi... Read More about Development of an inducible transposon system for efficient random mutagenesis in Clostridium acetobutylicum.

Improving the reproducibility of the NAP1/B1/027 epidemic strain R20291 in the hamster model of infection (2016)
Journal Article
Kelly, M. L., Ng, Y. K., Cartman, S. T., Collery, M. M., Cockayne, A., & Minton, N. P. (2016). Improving the reproducibility of the NAP1/B1/027 epidemic strain R20291 in the hamster model of infection. Anaerobe, 39, 51-53. https://doi.org/10.1016/j.anaerobe.2016.02.011

Comparative analysis of the Clostridium difficile BI/NAP1/027 strain R20291 and ClosTron-derived ermB mutants in the hamster infection model are compromised by the clindamycin susceptibility of the parent. Mutants can appear more virulent. We have re... Read More about Improving the reproducibility of the NAP1/B1/027 epidemic strain R20291 in the hamster model of infection.

Mutant generation by allelic exchange and genome resequencing of the biobutanol organism Clostridium acetobutylicum ATCC 824 (2016)
Journal Article
Ehsaan, M., Kuit, W., Zhang, Y., Cartman, S. T., Heap, J. T., Winzer, K., & Minton, N. P. (2016). Mutant generation by allelic exchange and genome resequencing of the biobutanol organism Clostridium acetobutylicum ATCC 824. Biotechnology for Biofuels, 9(1), Article 4. https://doi.org/10.1186/s13068-015-0410-0

Background Clostridium acetobutylicum represents a paradigm chassis for the industrial production of the biofuel biobutanol and a focus for metabolic engineering. We have previously developed procedures for the creation of in-frame, marker-less de... Read More about Mutant generation by allelic exchange and genome resequencing of the biobutanol organism Clostridium acetobutylicum ATCC 824.

Whole genome sequence and manual annotation of Clostridium autoethanogenum, an industrially relevant bacterium (2015)
Journal Article
Humphreys, C. M., McLean, S., Schatschneider, S., Millat, T., Henstra, A. M., Annan, F. J., …Minton, N. P. (2015). Whole genome sequence and manual annotation of Clostridium autoethanogenum, an industrially relevant bacterium. BMC Genomics, 16(1), Article 1085. https://doi.org/10.1186/s12864-015-2287-5

© 2015 Humphreys et al. Background: Clostridium autoethanogenum is an acetogenic bacterium capable of producing high value commodity chemicals and biofuels from the C1 gases present in synthesis gas. This common industrial waste gas can act as the so... Read More about Whole genome sequence and manual annotation of Clostridium autoethanogenum, an industrially relevant bacterium.

Clostridium difficile-mediated effects on human intestinal epithelia: Modelling host-pathogen interactions in a vertical diffusion chamber (2015)
Journal Article
Jafari, N. V., Kuehne, S. A., Minton, N. P., Allan, E., & Bajaj-Elliott, M. (2016). Clostridium difficile-mediated effects on human intestinal epithelia: Modelling host-pathogen interactions in a vertical diffusion chamber. Anaerobe, 37, 96-102. doi:10.1016/j.anaerobe.2015.12.007

Clostridium difficile infection is one of the leading causes of healthcare associated diarrhoea in the developed world. Although the contribution of C. difficile toxins to disease pathogenesis is now well understood, many facets of host-pathogen inte... Read More about Clostridium difficile-mediated effects on human intestinal epithelia: Modelling host-pathogen interactions in a vertical diffusion chamber.

A universal mariner transposon system for forward genetic studies in the genus Clostridium (2015)
Journal Article
Zhang, Y., Grosse-Honebrink, A., & Minton, N. P. (2015). A universal mariner transposon system for forward genetic studies in the genus Clostridium. PLoS ONE, 10(4), 1-21. https://doi.org/10.1371/journal.pone.0122411

DNA transposons represent an essential tool in the armoury of the molecular microbiologist. We previously developed a catP-based mini transposon system for Clostridium difficile in which the expression of the transposase gene was dependent on a sigma... Read More about A universal mariner transposon system for forward genetic studies in the genus Clostridium.

Coenzyme A-transferase-independent butyrate re-assimilation in Clostridium acetobutylicum - evidence from a mathematical model (2014)
Journal Article
Millat, T., Voigt, C., Janssen, H., Cooksley, C. M., Winzer, K., Minton, N. P., …Wolkenhauer, O. (2014). Coenzyme A-transferase-independent butyrate re-assimilation in Clostridium acetobutylicum - evidence from a mathematical model. Applied Microbiology and Biotechnology, 98(21), https://doi.org/10.1007/s00253-014-5987-x

The hetero-dimeric CoA-transferase CtfA/B is believed to be crucial for the metabolic transition from acidogenesis to solventogenesis in Clostridium acetobutylicum as part of the industrial-relevant acetone-butanol-ethanol (ABE) fermentation. Here, t... Read More about Coenzyme A-transferase-independent butyrate re-assimilation in Clostridium acetobutylicum - evidence from a mathematical model.

The role of flagella in Clostridium difficile pathogenesis:comparison between a non-epidemic and an epidemicstrain (2013)
Journal Article
Baban, S. T., Kuehne, S. A., Barketi-Klai, A., Cartman, S. T., Kelly, M. L., Hardie, K. R., …Minton, N. P. (2013). The role of flagella in Clostridium difficile pathogenesis:comparison between a non-epidemic and an epidemicstrain. PLoS ONE, 8(9), Article e73026. https://doi.org/10.1371/journal.pone.0073026

Clostridium difficile is a major cause of healthcare-associated infection and inflicts a considerable financial burden on healthcare systems worldwide. Disease symptoms range from self-limiting diarrhoea to fatal pseudomembranous colitis. Whilst C. d... Read More about The role of flagella in Clostridium difficile pathogenesis:comparison between a non-epidemic and an epidemicstrain.

Secretion and assembly of functional mini-cellulosomes from synthetic chromosomal operons in Clostridium acetobutylicum ATCC 824 (2013)
Journal Article
Kovacs, K., Willson, B. J., Schwarz, K., Heap, J. T., Jackson, A., Bolam, D. N., …Minton, N. P. (2013). Secretion and assembly of functional mini-cellulosomes from synthetic chromosomal operons in Clostridium acetobutylicum ATCC 824. Biotechnology for Biofuels, 6(117), https://doi.org/10.1186/1754-6834-6-117

BACKGROUND: Consolidated bioprocessing (CBP) is reliant on the simultaneous enzyme production, saccharification of biomass, and fermentation of released sugars into valuable products such as butanol. Clostridial species that produce butanol are, h... Read More about Secretion and assembly of functional mini-cellulosomes from synthetic chromosomal operons in Clostridium acetobutylicum ATCC 824.

Importance of Toxin A, Toxin B, and CDT in virulence of an epidemic Clostridium difficile strain (2013)
Journal Article
Kuehne, S. A., Collery, M. M., Kelly, M. L., Cartman, S. T., Cockayne, A., & Minton, N. P. (in press). Importance of Toxin A, Toxin B, and CDT in virulence of an epidemic Clostridium difficile strain. Journal of Infectious Diseases, 209(1), https://doi.org/10.1093/infdis/jit426

Clostridium difficile infection is the main cause of healthcare-acquired diarrhea in the developed world. In addition to the main virulence factors toxin A and B, epidemic, PCR Ribotype 027 strains, such as R20291, produce a third toxin, CDT. To deve... Read More about Importance of Toxin A, Toxin B, and CDT in virulence of an epidemic Clostridium difficile strain.

Clostridium difficile modulates host innate immunity via toxin-independent and dependent mechanism(s) (2013)
Journal Article
Jafari, N. V., Kuehne, S. A., Bryant, C. E., Elawad, M., Wren, B. W., Minton, N. P., …Bajaj-Elliott, M. (2013). Clostridium difficile modulates host innate immunity via toxin-independent and dependent mechanism(s). PLoS ONE, 8(7), Article e69846. https://doi.org/10.1371/journal.pone.0069846

Clostridium difficile infection (CDI) is the leading cause of hospital and community-acquired antibiotic-associated diarrhoea and currently represents a significant health burden. Although the role and contribution of C. difficile toxins to disease p... Read More about Clostridium difficile modulates host innate immunity via toxin-independent and dependent mechanism(s).

The putative influence of the agr operon upon survival mechanisms used by Clostridium acetobutylicum (2013)
Journal Article
Jabbari, S., Steiner, E., Heap, J. T., Winzer, K., Minton, N. P., & King, J. R. (2013). The putative influence of the agr operon upon survival mechanisms used by Clostridium acetobutylicum. Mathematical Biosciences, 243(2), https://doi.org/10.1016/j.mbs.2013.03.005

The bacterium Clostridium acetobutylicum produces acids as an energy-yielding process during exponential growth. An acidic environment, however, is toxic to the cells and two survival mechanisms are in place to prevent them from dying. Firstly, durin... Read More about The putative influence of the agr operon upon survival mechanisms used by Clostridium acetobutylicum.