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Myc and YY1 mediate activation of the Surf-1 promoter in response to serum growth factors (2000)
Journal Article
Vernon, E. G., & Gaston, K. (2000). Myc and YY1 mediate activation of the Surf-1 promoter in response to serum growth factors. BBA - Biochimica et Biophysica Acta, 1492(1), 172-179. doi:10.1016/S0167-4781(00)00116-0

The human Surf-1 and Surf-2 genes are divergently transcribed and share a single bi-directional promoter. The addition of serum growth factors to serum-starved cells activates transcription in the Surf-1 direction, but has no effect on transcription... Read More about Myc and YY1 mediate activation of the Surf-1 promoter in response to serum growth factors.

The HPV 16 E2 protein induces p53-dependent apoptosis (2000)
Presentation / Conference
Gaston, K., Webster, K., Roeder, G., & Parish, J. (2000, April). The HPV 16 E2 protein induces p53-dependent apoptosis. Paper presented at 10th TENOVUS Scotland Symposium: Gene Expression and Disease

The human papillomavirus (HPV) 16 E2 protein induces apoptosis in the absence of other HPV proteins and via a p53-dependent pathway (2000)
Journal Article
Webster, K., Parish, J., Pandya, M., Stern, P. L., Clarke, A. R., & Gaston, K. (2000). The human papillomavirus (HPV) 16 E2 protein induces apoptosis in the absence of other HPV proteins and via a p53-dependent pathway. Journal of Biological Chemistry, 275(1), 87-94. doi:10.1074/jbc.275.1.87

The human papillomavirus (HPV) E2 protein regulates viral gene expression and is also required for viral replication. HPV-transformed cells often contain chromosomally integrated copies of the HPV genome in which the viral E2 gene is disrupted. We ha... Read More about The human papillomavirus (HPV) 16 E2 protein induces apoptosis in the absence of other HPV proteins and via a p53-dependent pathway.

Magnesium ions enhance the transfer of Human Papillomavirus E2 protein from non-specific to specific binding sites (1999)
Journal Article
Lewis, H., & Gaston, K. (1999). Magnesium ions enhance the transfer of Human Papillomavirus E2 protein from non-specific to specific binding sites. Journal of Molecular Biology, 294(4), 885-896. doi:10.1006/jmbi.1999.3314

The human papillomavirus 16 E2 protein binds to four specific DNA sequences present within the HPV 16 genome and regulates viral gene expression and DNA replication. However, the E2 protein can also bind tightly to non-specific DNA sequences. Here, w... Read More about Magnesium ions enhance the transfer of Human Papillomavirus E2 protein from non-specific to specific binding sites.

Cellular transcirption factors regulate human papillomavirus type 16 gene expression by binding to a subset of the DNA sequences recognised by the viral E2 protein (1999)
Journal Article
Lewis, H., Webster, K., Sanchez-Perez, A., & Gaston, K. (1999). Cellular transcirption factors regulate human papillomavirus type 16 gene expression by binding to a subset of the DNA sequences recognised by the viral E2 protein. Journal of General Virology, 80(8), 2087-2096. doi:10.1099/0022-1317-80-8-2087

Human papillomavirus type 16 (HPV-16) is a DNA tumour virus that has been implicated in the development of cervical cancer. The HPV-16 E2 protein binds to four sites that are present upstream of the viral P97 promoter and regulates transcription of t... Read More about Cellular transcirption factors regulate human papillomavirus type 16 gene expression by binding to a subset of the DNA sequences recognised by the viral E2 protein.

Disruption of the human papillomavirus type 16 E2 gene protects cervical carcinoma cells from E2F-induced apoptosis (1997)
Journal Article
Sanchez-Perez, A., Soriano, S., Clarke, A. R., & Gaston, K. (1997). Disruption of the human papillomavirus type 16 E2 gene protects cervical carcinoma cells from E2F-induced apoptosis. Journal of General Virology, 78(11), 3009-3018. https://doi.org/10.1099/0022-1317-78-11-3009

Human papillomavirus type 16 (HPV-16) is a DNA tumour virus that has been implicated in the development of cervical cancer. In non-transformed HPV-infected cells, the HPV E2 protein regulates transcription of the viral E6 and E7 oncogenes. Malignant... Read More about Disruption of the human papillomavirus type 16 E2 gene protects cervical carcinoma cells from E2F-induced apoptosis.

A functional YY1 binding site is necessary and sufficient to activate Surf-1 promoter activity in response to serum growth factors (1997)
Journal Article
Cole, E. G., & Gaston, K. (1997). A functional YY1 binding site is necessary and sufficient to activate Surf-1 promoter activity in response to serum growth factors. Nucleic Acids Research, 25(18), 3705-3711. doi:10.1093/nar/25.18.3705

The human Surf-1 and Surf-2 housekeeping genes are divergently transcribed and share a bi-directional, TATA-less promoter. Housekeeping promoters typically contain complex arrays of transcription factor binding sites and several studies have suggeste... Read More about A functional YY1 binding site is necessary and sufficient to activate Surf-1 promoter activity in response to serum growth factors.

DNA binding and bending by the human papillomavirus type 16 E2 protein: recognition of an extended binding site (1997)
Journal Article
Thain, A., Webster, K., Emery, D., Clarke, A. R., & Gaston, K. (1997). DNA binding and bending by the human papillomavirus type 16 E2 protein: recognition of an extended binding site. Journal of Biological Chemistry, 272(13), 8236-8242. doi:10.1074/jbc.272.13.8236

The human papillomavirus (HPV) 16 E2 protein (hE2) binds to four sites present upstream of the P97 promoter and regulates transcription of the viral E6 and E7 oncogenes. We have determined the relative binding constants for the interaction of the ful... Read More about DNA binding and bending by the human papillomavirus type 16 E2 protein: recognition of an extended binding site.

CpG methylation directly inhibits binding of the human papillomavirus type-16 E2 protein to specific DNA sequences (1996)
Journal Article
Thain, A., Jenkins, O., Clarke, A. R., & Gaston, K. (1996). CpG methylation directly inhibits binding of the human papillomavirus type-16 E2 protein to specific DNA sequences. Journal of Virology, 70(10), 7233-7235. https://doi.org/10.1128/jvi.70.10.7233-7235.1996

CpG methylation of the human papillomavirus upstream regulatory region has previously been shown to reduce virus promoter activity. Here, we demonstrate that methylation of the CpG dinucleotides contained within the binding site of the human papillom... Read More about CpG methylation directly inhibits binding of the human papillomavirus type-16 E2 protein to specific DNA sequences.

CpG methylation and the binding of YY1 and ETC proteins to the Surf-1/Surf-2 bidirectional promoter (1995)
Journal Article
Gaston, K., & Fried, M. (1995). CpG methylation and the binding of YY1 and ETC proteins to the Surf-1/Surf-2 bidirectional promoter. Gene, 157(1-2), 257- 259. https://doi.org/10.1016/0378-1119%2895%2900120-U

The divergently transcribed Surf-1 and Surf-2 genes are separated by a bi-directional, TATA-less promoter which contains three important factor-binding sites, Sul, Su2 and Su3. The transcription initiation factor YY1 binds to the Sul site and stimula... Read More about CpG methylation and the binding of YY1 and ETC proteins to the Surf-1/Surf-2 bidirectional promoter.

The Surf-1 and Surf-2 genes and their essential bi-directional promoter elements are conserved between mouse and human (1994)
Journal Article
Lennard, A., Gaston, K., & Fried, M. (1994). The Surf-1 and Surf-2 genes and their essential bi-directional promoter elements are conserved between mouse and human. DNA and Cell Biology, 13(11), 1117-1126. https://doi.org/10.1089/dna.1994.13.1117

The organization of the Surfeit locus and the juxtaposition of at least five of the Surfeit genes (Surf?1 to ?5) are conserved between mouse and human (Williams et al., 1988; Yon et al., 1993). In the mouse, the heterogeneous transcription start site... Read More about The Surf-1 and Surf-2 genes and their essential bi-directional promoter elements are conserved between mouse and human.

YY1 is involved in the regulation of the bi-directional promoter of the Surf-1 and Surf-2 genes (1994)
Journal Article
Gaston, K., & Fried, M. (1994). YY1 is involved in the regulation of the bi-directional promoter of the Surf-1 and Surf-2 genes. FEBS Letters, 347(2-3), 289-294. https://doi.org/10.1016/0014-5793%2894%2900567-2

The Surfeit locus is an unusual cluster of at least 6 housekeeping genes whose organisation is conserved between birds and mammals. We have previously shown that the divergently transcribed Surf?1 and Surf?2 genes are separated by a bi?directional, T... Read More about YY1 is involved in the regulation of the bi-directional promoter of the Surf-1 and Surf-2 genes.

The isolation of transcription factors from ?gt11 cDNA expression libraries: human steroid 5?-reductase 1 has sequence-specific DNA binding activity (1992)
Journal Article
Gaston, K., & Fried, M. (1992). The isolation of transcription factors from ?gt11 cDNA expression libraries: human steroid 5?-reductase 1 has sequence-specific DNA binding activity. Nucleic Acids Research, 20(23), 6297-6301. https://doi.org/10.1093/nar/20.23.6297

The Surf-1/Surf-2 bi-directional promoter contains binding sites for at least three transcription factors (Su1, Su2, and Su3). By screening a ?gt11 HeLa cell cDNA expression library with a concatenated Su2 factor binding site, we isolated a cDNA whic... Read More about The isolation of transcription factors from ?gt11 cDNA expression libraries: human steroid 5?-reductase 1 has sequence-specific DNA binding activity.

A comparison of the DNA bending properties of the DNA binding proteins CRP and TFIID (1992)
Journal Article
Gaston, K., Bell, A., Busby, S., & Fried, M. (1992). A comparison of the DNA bending properties of the DNA binding proteins CRP and TFIID. Nucleic Acids Research, 20(13), 3391-3396. doi:10.1093/nar/20.13.3391

Protein-induced DNA bending is of importance in the formation of complex nucleoprotein assemblies such as those involved in the initiation of DNA replication or transcription initiation. We have compared the DNA bending characteristics of the Escheri... Read More about A comparison of the DNA bending properties of the DNA binding proteins CRP and TFIID.

The nirB promoter of Escherichia coli: location of nucleotide sequences essential for regulation by oxygen, the FNR protein and nitrite (1988)
Journal Article
Jayaraman, P., Gaston, K., Cole, J., & Busby, S. (1988). The nirB promoter of Escherichia coli: location of nucleotide sequences essential for regulation by oxygen, the FNR protein and nitrite. Molecular Microbiology, 2(4), 527-530. https://doi.org/10.1111/j.1365-2958.1988.tb00059.x

Using recombinant DNA techniques, nested deletions have been made upstream of the Escherichia coli nirB transcription start site and their effects on the regulation of nirB promoter activity have been measured. Nucleotide sequences downstream of ?73... Read More about The nirB promoter of Escherichia coli: location of nucleotide sequences essential for regulation by oxygen, the FNR protein and nitrite.