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Impact of the calcium form of ?-hydroxy-?-methylbutyrate upon human skeletal muscle protein metabolism (2017)
Journal Article
Wilkinson, D. J., Hossain, T., Limb, M. C., Phillips, B. E., Lund, J., Williams, J. P., …Atherton, P. J. (2018). Impact of the calcium form of β-hydroxy-β-methylbutyrate upon human skeletal muscle protein metabolism. Clinical Nutrition, 37(6), 2068-2075. https://doi.org/10.1016/j.clnu.2017.09.024

© 2017 The Authors Background & aims: β-hydroxy-β-methylbutyrate (HMB) is purported as a key nutritional supplement for the preservation of muscle mass in health, disease and as an ergogenic aid in exercise. Of the two available forms of HMB (calcium... Read More about Impact of the calcium form of ?-hydroxy-?-methylbutyrate upon human skeletal muscle protein metabolism.

Effects of leucine-enriched essential amino acid and whey protein bolus dosing upon skeletal muscle protein synthesis at rest and after exercise in older women (2017)
Journal Article
Wilkinson, D. J., Bukhari, S. S., Phillips, B. E., Limb, M. C., Cegielski, J., Brook, M. S., …Atherton, P. J. (2018). Effects of leucine-enriched essential amino acid and whey protein bolus dosing upon skeletal muscle protein synthesis at rest and after exercise in older women. Clinical Nutrition, 37(6), 2011-2021. https://doi.org/10.1016/j.clnu.2017.09.008

© 2017 The Authors Background & aims: Impaired anabolic responses to nutrition and exercise contribute to loss of skeletal muscle mass with ageing (sarcopenia). Here, we tested responses of muscle protein synthesis (MPS), in the under represented gr... Read More about Effects of leucine-enriched essential amino acid and whey protein bolus dosing upon skeletal muscle protein synthesis at rest and after exercise in older women.

A 4-week, lifestyle-integrated, home-based exercise training programme elicits improvements in physical function and lean mass in older men and women: a pilot study (2017)
Journal Article
Cegielski, J., Brook, M. S., Quinlan, J. I., Wilkinson, D. J., Smith, K., Atherton, P. J., & Phillips, B. E. (2017). A 4-week, lifestyle-integrated, home-based exercise training programme elicits improvements in physical function and lean mass in older men and women: a pilot study. F1000Research, https://doi.org/10.12688/f1000research.11894.2

Background: Developing alternative exercise programmes that can alleviate certain barriers to exercise such as psychological, environmental or socio-economical barriers, but provide similar physiological benefits e.g. increases in muscle mass and str... Read More about A 4-week, lifestyle-integrated, home-based exercise training programme elicits improvements in physical function and lean mass in older men and women: a pilot study.

Nutrient modulation in the management of disease-induced muscle wasting: evidence from human studies (2017)
Journal Article
Brook, M. S., Wilkinson, D. J., & Atherton, P. J. (2017). Nutrient modulation in the management of disease-induced muscle wasting: evidence from human studies. Current Opinion in Clinical Nutrition and Metabolic Care, 20(6), 433-439. https://doi.org/10.1097/MCO.0000000000000413

Purpose of review: In addition to being essential for movement, skeletal muscles act as both a store and source of key macronutrients. As such, muscle is an important tissue for whole body homeostasis, undergoing muscle wasting in times of starvation... Read More about Nutrient modulation in the management of disease-induced muscle wasting: evidence from human studies.

A novel D2O tracer method to quantify RNA turnover as a biomarker of de novo ribosomal biogenesis, in vitro, in animal models, and in human skeletal muscle (2017)
Journal Article
Brook, M. S., Wilkinson, D., Mitchell, W. K., Lund, J. N., Phillips, B. E., Szewczyk, N. J., …Atherton, P. J. (2017). A novel D2O tracer method to quantify RNA turnover as a biomarker of de novo ribosomal biogenesis, in vitro, in animal models, and in human skeletal muscle. AJP - Endocrinology and Metabolism, 313(6), Article E681-E689. https://doi.org/10.1152/ajpendo.00157.2017

Current methods to quantify in vivo RNA dynamics are limited. Here, we developed a novel stable isotope (D2O) methodology to quantify RNA synthesis (i.e., ribosomal biogenesis) in cells, animal models, and humans. First, proliferating C2C12 cells wer... Read More about A novel D2O tracer method to quantify RNA turnover as a biomarker of de novo ribosomal biogenesis, in vitro, in animal models, and in human skeletal muscle.

Human Skeletal Muscle Disuse Atrophy: Effects on Muscle Protein Synthesis, Breakdown, and Insulin Resistance—A Qualitative Review (2016)
Journal Article
Rudrappa, S. S., Wilkinson, D., Greenhaff, P. L., Smith, K., Idris, I., & Atherton, P. J. (2016). Human Skeletal Muscle Disuse Atrophy: Effects on Muscle Protein Synthesis, Breakdown, and Insulin Resistance—A Qualitative Review. Frontiers in Physiology, 7(361), https://doi.org/10.3389/fphys.2016.00361

The ever increasing burden of an ageing population and pandemic of metabolic syndrome worldwide demands further understanding of the modifiable risk factors in reducing disability and morbidity associated with these conditions. Disuse skeletal muscle... Read More about Human Skeletal Muscle Disuse Atrophy: Effects on Muscle Protein Synthesis, Breakdown, and Insulin Resistance—A Qualitative Review.

Historical and contemporary stable isotope tracer approaches to studying mammalian protein metabolism (2016)
Journal Article
Wilkinson, D. J. (in press). Historical and contemporary stable isotope tracer approaches to studying mammalian protein metabolism. Mass Spectrometry Reviews, https://doi.org/10.1002/mas.21507

Over a century ago, Frederick Soddy provided the first evidence for the existence of isotopes; elements that occupy the same position in the periodic table are essentially chemically identical but differ in mass due to a different number of neutrons... Read More about Historical and contemporary stable isotope tracer approaches to studying mammalian protein metabolism.

Relative Contribution of Intramyocellular Lipid to Whole-Body Fat Oxidation Is Reduced With Age but Subsarcolemmal Lipid Accumulation and Insulin Resistance Are Only Associated With Overweight Individuals (2016)
Journal Article
Chee, C., Shannon, C. E., Burns, A., Selby, A. L., Wilkinson, D., Smith, K., …Stephens, F. B. (2016). Relative Contribution of Intramyocellular Lipid to Whole-Body Fat Oxidation Is Reduced With Age but Subsarcolemmal Lipid Accumulation and Insulin Resistance Are Only Associated With Overweight Individuals. Diabetes, 65(4), 840-850. https://doi.org/10.2337/db15-1383

© 2016 by the American Diabetes Association. Insulin resistance is closely related to intramyocellular lipid (IMCL) accumulation, and both are associated with increasing age. It remains to be determined to what extent perturbations in IMCL metabolism... Read More about Relative Contribution of Intramyocellular Lipid to Whole-Body Fat Oxidation Is Reduced With Age but Subsarcolemmal Lipid Accumulation and Insulin Resistance Are Only Associated With Overweight Individuals.

Early structural remodeling and deuterium oxide-derived protein metabolic responses to eccentric and concentric loading in human skeletal muscle (2015)
Journal Article
Franchi, M. V., Wilkinson, D. J., Quinlan, J. I., Mitchell, W. K., Lund, J. N., Williams, J. P., …Narici, M. V. (2015). Early structural remodeling and deuterium oxide-derived protein metabolic responses to eccentric and concentric loading in human skeletal muscle. Physiological Reports, 3(11), Article e12593. https://doi.org/10.14814/phy2.12593

We recently reported that the greatest distinguishing feature between eccentric (ECC) and concentric (CON) muscle loading lays in architectural adaptations: ECC favors increases in fascicle length (Lf), associated with distal vastus lateralis muscle... Read More about Early structural remodeling and deuterium oxide-derived protein metabolic responses to eccentric and concentric loading in human skeletal muscle.

Internal comparison between deuterium oxide (D2O) and L-[ring-13C6] phenylalanine for acute measurement of muscle protein synthesis in humans (2015)
Journal Article
Smith, K., Cegielski, J., Wilkinson, D., Phillips, B., Boereboom, C., Lund, J., & Atherton, P. (2015). Internal comparison between deuterium oxide (D2O) and L-[ring-13C6] phenylalanine for acute measurement of muscle protein synthesis in humans. Physiological Reports, 3(7), https://doi.org/10.14814/phy2.12433

Stable isotope tracer methodologies are becoming increasingly widespread in metabolic research; yet a number of factors restrict their implementation, such as, i.v infusions, multiple cannulae, tissue samples, and significant cost. We recently valida... Read More about Internal comparison between deuterium oxide (D2O) and L-[ring-13C6] phenylalanine for acute measurement of muscle protein synthesis in humans.

Intake of low-dose leucine-rich essential amino acids stimulates muscle anabolism equivalently to bolus whey protein in older women at rest and after exercise (2015)
Journal Article
Bukhari, S. S., Phillips, B. E., Wilkinson, D. J., Limb, M. C., Rankin, D., Mitchell, W. K., …Atherton, P. J. (2015). Intake of low-dose leucine-rich essential amino acids stimulates muscle anabolism equivalently to bolus whey protein in older women at rest and after exercise. AJP - Endocrinology and Metabolism, 308(12), E1056-E1065. https://doi.org/10.1152/ajpendo.00481.2014

© 2015 American Physiological Society. All Rights Reseved. Dysregulated anabolic responses to nutrition/exercise may contribute to sarcopenia; however, these characteristics are poorly defined in female populations. We determined the effects of two f... Read More about Intake of low-dose leucine-rich essential amino acids stimulates muscle anabolism equivalently to bolus whey protein in older women at rest and after exercise.

Skeletal muscle hypertrophy adaptations predominate in the early stages of resistance exercise training, matching deuterium oxide-derived measures of muscle protein synthesis and mechanistic target of rapamycin complex 1 signaling (2015)
Journal Article
Brook, M. S., Wilkinson, D. J., Mitchell, W. K., Lund, J. N., Szewczyk, N. J., Greenhaff, P. L., …Atherton, P. (2015). Skeletal muscle hypertrophy adaptations predominate in the early stages of resistance exercise training, matching deuterium oxide-derived measures of muscle protein synthesis and mechanistic target of rapamycin complex 1 signaling. FASEB Journal, 29(11), https://doi.org/10.1096/fj.15-273755

Protein Carbonylation and Heat Shock Proteins in Human Skeletal Muscle: Relationships to Age and Sarcopenia (2014)
Journal Article
Beltran Valls, M. R., Wilkinson, D. J., Narici, M. V., Phillips, B. E., Smith, K., Caporossi, D., & Atherton, P. J. (2015). Protein Carbonylation and Heat Shock Proteins in Human Skeletal Muscle: Relationships to Age and Sarcopenia. Journals of Gerontology, Series A, 70(2), 174-181. https://doi.org/10.1093/gerona/glu007

Aging is associated with a gradual loss of muscle mass termed sarcopenia, which has significant impact on quality-of-life. Because oxidative stress is proposed to negatively impact upon musculoskeletal aging, we investigated links between human aging... Read More about Protein Carbonylation and Heat Shock Proteins in Human Skeletal Muscle: Relationships to Age and Sarcopenia.

A validation of the application of D2O stable isotope tracer techniques for monitoring day-to-day changes in muscle protein subfraction synthesis in humans (2013)
Journal Article
Wilkinson, D. J., Franchi, M. V., Brook, M. S., Narici, M. V., Williams, J. P., Mitchell, W. K., …Smith, K. (2014). A validation of the application of D2O stable isotope tracer techniques for monitoring day-to-day changes in muscle protein subfraction synthesis in humans. AJP - Endocrinology and Metabolism, 306(5), Article E571-E579. https://doi.org/10.1152/ajpendo.00650.2013

Quantification of muscle protein synthesis (MPS) remains a cornerstone for understanding the control of muscle mass. Traditional [13C]amino acid tracer methodologies necessitate sustained bed rest and intravenous cannulation(s), restricting studies t... Read More about A validation of the application of D2O stable isotope tracer techniques for monitoring day-to-day changes in muscle protein subfraction synthesis in humans.