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Coiled coil type neoglycoproteins presenting three lactose residues (2016)
Journal Article
Sweeney, S. M., Bullen, G. A., Gillis, R. B., Adams, G. G., Rowe, A. J., Harding, S. E., …Murphy, P. V. (2016). Coiled coil type neoglycoproteins presenting three lactose residues. Tetrahedron Letters, 57(13), https://doi.org/10.1016/j.tetlet.2016.02.005

Scaffold design, synthesis and application are relevant for biomedical research. For example, multivalent interactions, such as those between cell surface glycoproteins and lectins can influence the potency and duration of signalling. The spacing bet... Read More about Coiled coil type neoglycoproteins presenting three lactose residues.

Location dependent coordination chemistry and MRI relaxivity, in de novo designed lanthanide coiled coils (2015)
Journal Article
Berwick, M. R., Slope, L. N., Smith, C. F., King, S. M., Newton, S. L., Gillis, R. B., …Peacock, A. F. A. (in press). Location dependent coordination chemistry and MRI relaxivity, in de novo designed lanthanide coiled coils. Chemical Science, 7(3), https://doi.org/10.1039/c5sc04101e

Herein, we establish for the first time the design principles for lanthanide coordination within coiled coils, and the important consequences of binding site translation. By interrogating design requirements and by systematically translating binding... Read More about Location dependent coordination chemistry and MRI relaxivity, in de novo designed lanthanide coiled coils.

Ultracentrifuge Methods for the Analysis of Polysaccharides, Glycoconjugates, and Lignins (2015)
Book Chapter
Harding, S. E., Adams, G. G., Almutairi, F., Alzahrani, Q., Erten, T., Samil Kök, M., & Gillis, R. B. (2015). Ultracentrifuge Methods for the Analysis of Polysaccharides, Glycoconjugates, and Lignins. In Analytical Ultracentrifugation (391-439). Academic Press. https://doi.org/10.1016/bs.mie.2015.06.043

Although like proteins, polysaccharides are synthesized by enzymes, unlike proteins there is no template. This means that they are polydisperse, do not generally have compact folded structures, and are often very large with greater nonideality behavi... Read More about Ultracentrifuge Methods for the Analysis of Polysaccharides, Glycoconjugates, and Lignins.

Matrix-free hydrodynamic study on the size distribution and conformation of three technical lignins from wood and non-wood (2015)
Journal Article
Alzahrani, Q. E., Adams, G. G., Gillis, R. B., Besong, T. M., Kök, M. S., Fong, E., …Harding, S. E. (2016). Matrix-free hydrodynamic study on the size distribution and conformation of three technical lignins from wood and non-wood. Holzforschung, 70(2), https://doi.org/10.1515/hf-2014-0318

Molecular weight (MW) and related conformational data of three commercially available technical lignins (Alcell L, kraft L, and soda L) have been studied by means of analytical ultracentrifugation, taking advantage of some recent developments in both... Read More about Matrix-free hydrodynamic study on the size distribution and conformation of three technical lignins from wood and non-wood.

Recent advances in the analysis of macromolecular interactions using the matrix-free method of sedimentation in the analytical ultracentrifuge (2015)
Journal Article
Harding, S., Gillis, R., Almutairi, F., Erten, T., Kök, M., & Adams, G. (2015). Recent advances in the analysis of macromolecular interactions using the matrix-free method of sedimentation in the analytical ultracentrifuge. Biology, 4(1), https://doi.org/10.3390/biology4010237

Sedimentation in the analytical ultracentrifuge is a matrix free solution technique with no immobilisation, columns, or membranes required and can be used to study self-association and complex or “hetero”-interactions, stoichiometry, reversibility an... Read More about Recent advances in the analysis of macromolecular interactions using the matrix-free method of sedimentation in the analytical ultracentrifuge.

A review of modern approaches to the hydrodynamic characterisation of polydisperse macromolecular systems in biotechnology (2015)
Journal Article
Gillis, R. B., Rowe, A. J., Adams, G. G., & Harding, S. E. (2015). A review of modern approaches to the hydrodynamic characterisation of polydisperse macromolecular systems in biotechnology. Biotechnology and Genetic Engineering Reviews, 30(2), https://doi.org/10.1080/02648725.2014.994870

This short review considers the range of modern techniques for the hydrodynamic characterisation of macromolecules – particularly large glycosylated systems used in the food, biopharma and healthcare industries. The range or polydispersity of molecul... Read More about A review of modern approaches to the hydrodynamic characterisation of polydisperse macromolecular systems in biotechnology.

Characterization of capsular polysaccharides and their glycoconjugates by hydrodynamic methods (2015)
Book Chapter
Harding, S. E., Abdelhameed, A. S., Gillis, R. B., Morris, G. A., & Adams, G. G. (2015). Characterization of capsular polysaccharides and their glycoconjugates by hydrodynamic methods. In Carbohydrate-based vaccines (211-227). Springer. https://doi.org/10.1007/978-1-4939-2874-3_13

Hydrodynamic methods are relevant for the characterization of carbohydrates such as capsular bacterial polysaccharides or glycoconjugates in solution. This chapter focuses on the following hydrodynamic methods: sedimentation velocity analytical ultra... Read More about Characterization of capsular polysaccharides and their glycoconjugates by hydrodynamic methods.

Protein-like fully reversible tetramerisation and super-association of an aminocellulose (2014)
Journal Article
Nikolajski, M., Adams, G. G., Gillis, R. B., Besong, D. T., Rowe, A. J., Heinze, T., & Harding, S. E. (2014). Protein-like fully reversible tetramerisation and super-association of an aminocellulose. Scientific Reports, 4, Article 3861. https://doi.org/10.1038/srep03861

Unusual protein-like, partially reversible associative behaviour has recently been observed in solutions of the water soluble carbohydrates known as 6-deoxy-6-(?-aminoalkyl)aminocelluloses, which produce controllable self-assembling films for enzyme... Read More about Protein-like fully reversible tetramerisation and super-association of an aminocellulose.

MultiSig: a new high-precision approach to the analysis of complex biomolecular systems (2013)
Journal Article
Gillis, R. B., Adams, G. G., Heinze, T., Nikolajski, M., Harding, S. E., & Rowe, A. J. (2013). MultiSig: a new high-precision approach to the analysis of complex biomolecular systems. European Biophysics Journal, 42(10), https://doi.org/10.1007/s00249-013-0924-y

MultiSig is a newly developed mode of analysis of sedimentation equilibrium (SE) experiments in the analytical ultracentrifuge, having the capability of taking advantage of the remarkable precision (~0.1 % of signal) of the principal optical (fringe)... Read More about MultiSig: a new high-precision approach to the analysis of complex biomolecular systems.